D3S1358


Other Names
Chromosomal Location
GenBank Accession
 
3p21

11449919; has 18 repeats

TCTA(TCTG)2(TCTA)15

Repeat: [AGAT], [TCTA]= bottom strand
 

Reported Primers Ref. PCR Primer Sequences
Set 1
148, 502
5'-ACT GCA GTC CAA TCT GGG T-3' (AGAT strand)

5'-ATG AAA TCA ACA GAG GCT TG-3' (TCTA strand)

Set 2
ABI AmpFlSTR® Profiler Plus™
Set 3  Promega GenePrint® PowerPlex™ 2.1

PCR Product Sizes of Observed Alleles
 

Allele       (Repeat #)
Set 1,3
Set 2
Repeat Structure
Ref.
8 99 bp 97 bp  

variant allele

9 103 bp 101 bp   SGM Plus
10 107 bp 105 bp   SGM Plus
11 111 bp 109 bp   SGM Plus
12 115 bp 113 bp   SGM Plus
13 119 bp  117 bp  TCTA[TCTG]2[TCTA]10 729
14 123 bp 121 bp TCTA[TCTG]2[TCTA]11 668
15 127 bp 125 bp TCTA[TCTG]3[TCTA]11 668
15' 127 bp 125 bp TCTA[TCTG]2[TCTA]12  
15.2 129 bp 127 bp   SGM Plus
16 131 bp 129 bp TCTA[TCTG]3[TCTA]12 668
16' 131 bp 129 bp TCTA[TCTG]2[TCTA]13 729
16.2 133 bp 131 bp   642
17 135 bp 133 bp TCTA[TCTG]3[TCTA]13 668
17' 135 bp 133 bp TCTA[TCTG]2[TCTA]14 729
17.1 136 bp 134 bp   SGM Plus
18 139 bp 137 bp TCTA[TCTG]3[TCTA]14 668
18.3 142 bp 140 bp  

variant allele

19 143 bp 141 bp TCTA[TCTG]3[TCTA]15 729
20  147 bp 145 bp   729

Allelic Ladders: Commercially available from Applied Biosystems (ladder allele range: 12-19)

Common Multiplexes: AmpFlSTR Blue, PwerPlex 1.1, Profiler, Profiler Plus, and COfiler
 
 

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