Page last updated: 07/25/2017

D16S539 Variants


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Allele Designation

Allele Size 

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

4

257.74

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

6

237.30

ABI 377

CO

Nicole Swinton

Re-extracted and reamplified

 

 

6 [2]

233.11

ABI 310

PS,CO

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

6 [3]

233.44

ABI 310

CO

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

 

6 [4]

264.8

ABI 3130

PP 16

Jason Hodges, WV State Police Crime Lab

Re-amplified

Convicted Offender

 

6 [5]

256.03

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

Re-amplified

 

 

7

237.50

ABI 310

CO

Russell Vossbrink

Observed in 1 k and 3 q's

Casework

 

7 [2]

269.98

ABI 3100

PP

Cory Formea, IL State Police DNA Indexing Lab

Re-amplification

Convicted Offender Sample

1

7 [3]

260.10

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

Re-amplified

 

 

7 [4] 261 ABI 310 ID

Jiri Drabek, Czech forensic genetics expert, Olomouc

pedigree (mother 7,12 and child 7,12)

Paternity case. Frequency (number on times observed): twice (mother and child); the largest set of the published Czech population frequencies (Simkova et al., 2009) had number of alleles=2822 for D16S539 without allele 7 encountered, so p=1/(2822+1)=0.000354233.

 

8.3

267.94

ABI 310

ID

Angelo Abati, Institut de medecine legale de  LIEGE Belgium

3 extractions, 3 PCR in Identifilier and 3 PCR in PP16 and confirmation with the father profile which has the same allele

 

1 in 800 convicted offenders (+ the father)

8.3 [2]

277.45

ABI 310

PP16

Angelo Abati, Institut de medecine legale de  LIEGE Belgium

3 extractions, 3 PCR in Identifilier and 3 PCR in PP16 and confirmation with the father profile which has the same allele

 

1 in 800 convicted offenders (+ the father)

9.3

248.29

ABI 310

CO

MO State Highway Patrol

Re-extracted and re-amplified

Convicted Offender

1 in 4514 to date

9.3 [2]

271.71

ABI 310

ID

Angelo ABATI,

Belgium

3 times in ID

 

1 in 1100

9.3 [3]

281.26

ABI 310

PP 16

Angelo ABATI,

Belgium

3 times in PP16

 

1 in 1100

9.3 [4]

271.37

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

9.3 [5]

248.02

ABI 3100

PS

Angela Withrow        M U F S C

 

 

 

9.3 [6] 271.52 ABI 310 ID Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Criminal case sample  

10.1

283.01

ABI 3130XL

PP

Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory

Confirmed with multiple samples

 

 

10.1 [2] 294.54 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
10.2 273.18 ABI 3130 ID Amy Jagmin, Washington State Patrol Crime Laboratory present in two different samples, re-injected samples were mixed and variant allele in each was a minor contributor 2

10.3

275.07

ABI 3100

ID

Marsh Garcia, Orchid Cellmark, Nashville  TN

Re-injected

 

 

10.3 [2]

275.07

ABI 3100

ID

Marsh Garcia, Orchid Cellmark, Nashville  TN

Observed in multiple samples, re-injected

 

 

10.3 [3]

274.9

ABI 3100

ID

Marsh Garcia, Orchid Cellmark, Nashville  TN

Observed in multiple samples, re-injected

 

 

10.3 [4] 278.16 ABI 3730 ID DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-analyzed with ABI 3730   2 in 31,330
10.3 [5] 296.10 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)

11.1

287.01

ABI 3100

PP

Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory

Re-Extraction

 

 

11.3

256.27

ABI 310

CO

David Young

Texas DPS

Multiple amplifications

Evidence samples

1 in 7500 to date

11.3 [2]

279.13

ABI 3100

ID

Angela Withrow

M U F S C

Re-amplified

Alabama Depart of Forensic Sciences Convicted Offender

3 in 12115

11.3 [3]

288.85

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

11.3 [4]

279.25

ABI 3100

ID

Paul Mazur

Erie County CPS Forensic Lab

Buffalo, NY

Multiple amplifications

Casework sample

 

11.3 [5]

278.96

ABI 3100

ID

Angela Withrow         M U F S C

 

 

 

11.3 [6]

278.96

ABI 3100

ID

Angela Withrow         M U F S C

 

 

 

11.3 [7] 279.22 ABI 3130 ID DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-analyzed with ABI 3130   2 in 31,330
11.3 [8] 280.04 ABI 3730 ID DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-analyzed with ABI 3730   1 in 31,330
11.3 [9] 300.69 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Crime scene sample 1 (first observation)

12.1

258.39

ABI 310

CO

Nicole Swinton

Re-extracted and re-amplified

 

 

12.1 [2]

293.04

ABI 310

PP 1.2

Greg Sykes

 ATCC

Re-extracted and Re-amplified

From a colon carcinoma with integrated CMV-promoter-driven GADD45a

expression vector, ATCC cell line CRL-2579; name:  RKO-AS45-1

 

12.1 [3]

290.85

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

12.1 [4]

290.98

ABI 3100

PP

ICMP

Re-extraction and amplification or observed intergenerational inheritance

Blood reference

2 in 11000

12.1 [5]

281.42

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

 

 

 

12.1 [6] 281.65 ABI 3130xl ID Direct Nelson Gaburo Junior - Department of Molecular Biology - Diagnosticos da America - DASA Re-amplified and Re-analyzed    
12.1 [7] 302.31 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Crime scene sample 1 (first observation)

12.2

263.17

ABI 377

CO

Kelly Solis

Texas DPS

Re-amplified

Convicted offender

1 in 35000

12.3

283.46

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

Re-amplified

 

 

12.3 [2] 304.33 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)

13.1

297.18 (POP-4)

299.84 (POP-6)

ABI 310

PP

Greg Sykes

ATCC

Re-amplified three times and run using high resolution POP-6 polymer

allele discovered in a colon carcinoma cell line transfected with HPV E6 inserted in pCMV.3 (name designation: RKO-E6)

 

13.2

286.15

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

 

Re-injected

 

 

13.2 [2] 286.62; 286.57 ABI 3130xl ID Christine Barnes, St. Louis Metropolitan Police Dept, St. Louis, MO Re-amplified OL observed in multiple evidence samples  

13.3

267.93

ABI 377

CO, PS

Nicole Swinton

Re-extracted and re-amplified

 

 

13.3 [2]

287.18

ABI 3100

ID

Angela Withrow

M U F S C

Re-amplified

Alabama Depart of Forensic Sciences Convicted Offender

2 in 12,812

13.3 [3]

296.97

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 33739

13.3 [4]

287.65 +/-0.18

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

4 in 11500

13.3 [5]

264.47

ABI 3100

CO

Angela Withrow   

M U F S C

Previously seen - not verified

 

3

13.3 [6]

264.34

ABI 3100

CO

Nicole Hahn

M U F S C

Previously Seen – no verification

 

4

13.3 [7]

264.47

ABI 3100

CO

Nicole Hahn

M U F S C

Previously Seen – no verification

 

5

13.3 [8] 286.53 ABI 3500 Identifiler Plus Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender  
13.3 [9] 286.47 ABI 3500 ID Plus Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender  
13.3 [10] 287.02 (1), 287.04 (2), 287.03 (3) ABI 3130 ID Brenda Crosby, St. Louis Metropolitan Police Department Crime Lab Re-amplified and detected in multiple intimate samples from the same individual    

14.1

308.45

ABI 3100

PPX16

Marsha Garcia, Orchid Cellmark, Nashville  TN

Re-amplified

 

 

14.2

290.27

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville  TN

re-injected

 

 

14.3

272.02

ABI 337

CO

Nicole Swinton

Re-extracted and re-amplified

 

 

16

273.57

ABI 310

CO

Carolyn Booker

Reproduced in blood, saliva, hair, and fingernail samples

Discovered during validation testing

One individual

16 [2]

273.81±0.10

ABI 3100

CO

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

2 in 3750

16 [3]

306.10

ABI 3100

PP

ICMP

Re-extraction and amplification or observed intergenerational inheritance

Blood reference

1 in 11000

16 [4]

296.29

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

Re-extracted

 

 

16 [5]

273.56 (average of two injections)

ABI 310

CO

Andrew Garinger, Illinois State Police

 

Observed in both victim standard and sample F1.

 

16 [6] 127.55 ABI 3500 PP21 Massimo Mangiola, Rhode Island Blood Center Re-amplified & re-analyzed Immigration case (AM+CH) 2:1128

16 (>15)

305.96

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

17 277 ABI 3130 SGM+ Rivka Adelman, Israeli Police Observed in 4 PCR of 3 items and 1 Reference (the same allele size) Casework  

22 reported variants as of 08/04/2016

 

Tri-Allelic Patterns

Allele Designation

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

7, 11, 12

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract & Re-amplify

Convicted Offender

1 in 69600

9, 9.2, 11 ABI 3130 PP16HS Compton Beecher, Caribbean Genetics Kingston 7 Jamica Duplicate 9.2 Allele half peak heights of other alleles 2
9, 10, 11 ABI 3130xl ID Robert Dorion, Washington State Patrol Crime Laboratory Re-extraction Convicted Offender  
9, 10, 12 ABI 310 ID Kelly Pokorny & Kyra Groeblinghoff, St. Louis County Police Crime Laboratory Reinjection, re-amplification and re-extraction Allele 10 repeatedly amplifies at much lower RFU than 9,12 alleles 1
9, 10, 12 [2] ABI 3500 ESI 16 Horolma Pamjav, Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA Laboratory, Budapest, Hungary   Observed in Hungarian population data 1 in 21,473

9, 11, 12

ABI 3100

ID

Angela Withrow

 M U F S C

Re-amp x 1

AL Dept of Forensic Sciences Convicted Offender Sample

1 in 15144

9, 11, 12 [2] ABI 3730 ID DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-amplified and re-analyzed with ABI 3730   1 in 31,330
9, 11, 12 [3] ABI 3130 ID Pamela Larkin, St. Louis Metropolitan Police Department Crime Lab Re-amplified Reference sample 1
9, 13, 14 ABI 3130xl ID Robert Dorion, Washington State Patrol Crime Lab Re-extraction Convicted offender  
9.2, 11, 12 ABI 310 PP16HS Ashley Hayes, Ann Arundel Co. Police Dept. Crime Lab, MD Re-extracted   1

10, 12, 13

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

10, 12, 14.2

ABI 3130xl

 

Robert Dorion, Washington State Patrol Crime Laboratory

 

 

 

11, 12, 13

ABI 3100

ID

Angela Withrow     M U F S C

Re-amp x 1

AL Dept of Forensic Sciences Convicted Offender Sample

1 in 14767

11, 12, 13 [2]

ABI 310

PP 16

Anthony J. Tambasco, Mansfield, Ohio, Police Lab

Re-injection

Two seperate crime scenes and suspect standard

1

11, 12, 13 [3] ABI 310 ID Plus Winnie Kurowski, Acadiana Criminalistics Lab, New Iberia, LA Re-amplified, re-injected Reference sample N/A

11, 13, 14

ABI 3100

ID

Angela Withrow     M U FS  C

Two extractions/Three amps

 

1 in 12812

11, 13, 14 [2]

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract & Re-amplify

Convicted Offender

1 in 69600

11, 13, 14 [3] ABI 3500 ESI 16

Horolma Pamjav, Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA Laboratory, Budapest, Hungary

  Observed in Hungarian population data 1 in 21,473

12, 13, 14

ABI 377

CO

Karl Morton

Texas DPS

 

Re-extracted 

Convicted offender

1 in 39,000

12, 13, 14 [2]

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract & Re-amplify

Convicted Offender

1 in 69600

12, 14, 15 ABI 3130 ID Kristen Homich, Erie County Central Police Services
Forensic Laboratory, Buffalo  NY
Re-amplification and electrophoresis   1

13 reported tri-allelic patterns as of 08/04/2016

 

* Use the following abbreviations for amplification kit names:

 

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Last Updated: 08/04/2016