Page last updated: 11/13/2017

D1S1656 Variants


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Allele Designation

Allele Size

Instrument

Amp Kit*

Contributor

Verification/ Confirmation Method(s)

Notes

Frequency

7 212.4 bp (ESI)

122.4 bp (ESX)

ABI 3130xl PowerPlex ESI 16 and ESX 16 Naftaly Einot, Carla Oz, Moshe Spitzen, Shlomit Avraham; Israel Police DNA Biology Lab, Jerusalem, Israel

Multiple extractions from separate samples (Casework and Reference samples), Amplified with ESI and ESX

 

observed in two completely different profiles  
7 (<8) [2] 122.69 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
7 (<8) [3] 122.77 (ESX 16) and 212.35 (EXI 16) ABI 3500xl PowerPlex ESX 16 Fast and ESI 16 Malin Sanga, Swedish National Forensic Centre (NFC) Re-amplified with ESX 16 and ESI 16 and re-electrophoresed Reference sample 1 (first observation)
7 (<9) [4]151.86;147.32ABI 3500xlGlobalfiler;Powerplex 21Krystle Oliveire, DNA Analyst. Trinidad and Tobago Forensic Science CentreUpon running both the reference sample of the victim and the crime scene sample, using Powerplex 21, an off ladder peak was noted directly between D3 and D1 for the duplicate of the reference as well as the crime scene. It was not a PCR or eletrophoretic artifact upon analysis of the profiles. In order to determine which locus the allele belonged to, Globalfiler was used to run a duplicate of the reference sample. With Globafiler, the same off ladder showed up between D10 and D1, closer to the end of the D1 locus. For this reason, it was determined that the allele belonged to the D1 locus and was smaller than allele 9 which is the lowest designation on the ladder for that particular locus. It appears to be a possible allele 7 when extrapolation of sizing data using the different ladders and kits and runs were performed. This is just an estimate however and can never be truly known unless sequencing is performed.First time observed in casework.
8 169.5 ABI 3130, POP-4 NGM SElect Peter Schneider, Institute of Legal Medicine, University of Cologne, Germany analysis repeated reference sample, European ancestry once
10.3 138.15 ABI 3500xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
11.1 140.24 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
11.3 142.21 ABI 3500xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
12.1 170.64 ABI 3500 PP21 Denise Grover, NT Forensic Biology, Australia Re-amplification   1
12.3 146.19 ABI 3500xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
12.3 [2] 172.66 ABI 3500 PP21 Francois Chatenay-Lapointe, Rhode Island Blood Center Re-amplified and re-analyzed Immigration case 2 in 2986
13.1 148.52 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Crime scene sample 1 (first observation)
13.3 192.50 ABI 3500 NGM SElect Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
13.3 [2] 192.61 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
13.3 [3] 150.29 ABI 3500xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
13.3 [4] 176.65 ABI 3500 PP21 Massimo Mangiola, Rhode Island Blood Center Re-amplified & re-analyzed Immigration case (AM+CH) 2 in 2730
14.1 242.01 ABI 3500xl ESI Liron Elkayam, Carla Oz, Israel Police DNA Database Re-extracted and re-amplified in duplicate Reported size is average of 242.03 bp and 241.99 bp 1 sample
14.2 153.55 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
15.1 156.58 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
15.2 246.82, 246.73 ABI 3500 ESI Carla Oz, Israel Police DNA Biology Lab, Jerusalem, Israel Extractions from separate samples (casework samples)   2
15.2 [2] 199.47 ABI 310 NGM SElect Ioulia Skitsa and Alexandra Ampati, DNA Analysis Laboratory, Athens Legal Medicine Department, Greece Reference sample and vaginal/rectal samples (3 different extractions) Individual from Russia 1
15.2 [3] 157.77 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Crime scene sample 1 (first observation)
16.1 186.77 ABI 3500 PP21 Denise Grover, NT Forensic Biology, Australia Re-amplification   1
16.1 [2] 160.72 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
16.1 [3]187.42ABI 3500xlPowerPlex Fusion 6CBrendan Shanahan, Washington State Patrol Crime LaboratoryRe- direct amplificationConvicted Offender
16.2 204.75 bp, 204.84 bp ABI 3130xl NGM Naomi Zaken, Carla Oz, Ashira Zamir,  Israel Police DNA Database, Jerusalem, Israel Re-extracted and re-amplified, in duplicate Samples on FTA cards 1 sample
16.2 [2] 203.35 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Criminal case sample, no suspect  
16.2 [3] 161.56 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
17.1 206.30 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Criminal case sample, no suspect  
17.1 [2] 253.92 (ESI 17); 193.35 (Globalfiler) ABI 3130xl PowerPlex ESI 17 & Globalfiler Express Paulo Dario, Carina Almeida, Ana Rita Oliveira and Teresa Ribeiro, National Institute of Legal Medicine and Forensic Sciences - South Branch, Portugal Kit overlap, two different samples collected (blood and saliva) and analyzed with different kits Individual from the South of Portugal 1
17.1 [3] 164.73 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
17.2 165.61 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
18 209.31 ABI 3500 NGM SElect Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
18 [2] 209.26 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
18 [3] 209.34 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
18 [4] 209.28 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
18 [5] 209.25 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
18 [6] 209.38 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
18 [7] 209.40 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
18 [8] 209.33 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
18 [9] 209.40 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Criminal case sample, no suspect  
18.1 168.74 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
18.1 [2]197.31-197.39ABI 3500Applied Biosystems GlobalFilerSabrina Seehafer, DCI Crime Laboratory, Ankeny, IARe-amplified in GlobalFiler & seen in question profiles & known buccal profileknown buccal sample & question profiles3
18.2 169.65 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
19 213.23 ABI 3500 NGM SElect Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
19 [2] 213.31 ABI 3500 NGM SElect Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Criminal case sample, no suspect  
19 [3] 213.45 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
19 [4] 213.29 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Criminal case sample, no suspect  
20 201.69 ABI 3500 PP21 Denise Grover, NT Forensic Biology, Australia Re-amplification   1
20 [2] 175.64 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
>21 182.71 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Crime scene sample 1 (first observation)
21.3 211.32 ABI 3500 GlobalFiler Hortensia Cano, Valeria Marcucci. Laboratorio de Investigación Forense. Santa Cruz-Argentina Re-extraction and re-amplified with GlobalFiler and re-amplified with PowerPlex Fusion Reference sample 1 (first observation)

24 reported variants as of 11/15/2017

 

Tri-Allelic Patterns

Allele Designation Instrument Amp Kit* Contributor Verification/Conformation Method(s) Notes Frequency
12, 13, 15.3 ABI 3500 ESI 16

Horolma Pamjav, Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA Laboratory, Budapest, Hungary

  Observed in Hungarian population data 1 in 21,473
15.3, 16, 17 ABI 3500 ESI 16

Horolma Pamjav, Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA Laboratory, Budapest, Hungary

  Observed in Hungarian population data 1 in 21,473
16, 17, 18.3 ABI 3500 ESI 16

Horolma Pamjav, Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA Laboratory, Budapest, Hungary

  Observed in Hungarian population data 1 in 21,473

3  reported tri-allelic patterns as of 02/23/2017

* Use the following abbreviations for amplification kit names:

  • CO: PE Applied Biosystems COfilerTM

  • PR: PE Applied Biosystems ProfilerTM

  • PS: PE Applied Biosystems Profiler PlusTM

  • ID: PE Applied Biosystems Indetifiler™

  • CV: Promega CTTVTM

  • CA: Promega CTTATM

  • PP: Promega PowerPlexTM

  • MP: Promega MonoPlexTM

  • Other: Please specify

 

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