Page last updated: 07/25/2017

D2S441 Variants


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Allele Designation

Allele Size

Instrument

Amp Kit*

Contributor

Verification/ Confirmation Method(s)

Notes

Frequency

<8 84.30 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Crime scene sample 1 (first observation)
<8 [2]

(called as a 20.3 allele in D1S1656; most likely contains 7 D2S441 repeats)

204.89 ABI 3500xl PowerPlex Fusion Alyse Yacovone-Margetts, Palm Beach County Sheriff's Office Utilized the allelic ladder to determine the base pair difference between the called allele in D1S1656 and the smallest allele, from the allelic ladder, in D2S441. The sample was not reamplified or reinjected, however the same pattern was observed in evidence samples. This allele was called as a 20.3 microvariant within the D1S1656 locus. However, based on the fact that the locus already was called a heterozygote (14,16) and that tri-allelic patterns usually do not have a wide a repeat range as this would have been, it was investigated to see if this allele could be smaller than the smallest allele in the Fusion kit for D2S441. Using an allelic ladder, it was determined that the called peak of 20.3, 204.89 bp was exactly 4.02 bp smaller than the smallest allele (8; 208.91 bp) in the kit allelic ladder for this marker. 1
8 76.34 ABI 3130xl NGM

Nelson Gaburo Junior, Luciano Cesar Scarpelli, Daniela Moratori, Pamela de Melo Machado, Carla Naufal Fontes, Renato Bueno de Moraes, Maria Cristina Sales, Tamyrez Ayrez Marques - Department of Molecular Biology – Diagnósticos da América - DASA

Re-amplified and re-analyzed Observed in paternity identification  
8.1 89.18 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
9.1 346 (ESI 17)

81 (NGM SElect)

ABI 3130xl NGM SElect and PowerPlex ESI 17 M.llo De Meo RIS ROMA      
9.1 [2] 93.30 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Crime scene sample 1 (first observation)
9.3 349.26 ABI 3500 ESI kit Margalit Sin David, Israel Police DNA Database, Jerusalem, Israel Re-extracted and re-amplified    
9.3 [2] 95.58 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
10.1 351.42 ABI 3500xl ESI Sivan Mishan, Liron Elkayam, Carla Oz, Israel Police DNA Database Re-extracted and re-amplified reported size is average of 251.44 bp and 351.40 bp 2 samples
10.1 [2] 97.47 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
10.3 220.60 ABI 3500 PowerPlex Fusion

Paola Zanelli, Stefania Bardini, Silvia Giuliodori, Magda Benecchi, Valentina Sgobba –

Immunogenetics Laboratory, Parma University Hospital

Re-extracted and re-amplified    
10.3 [2] 99.47 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Crime scene sample 1 (first observation)
11.1 101.31 ABI 3500 PowerPlex ESX 17 and Fusion

Docteur Angelo ABATI

Responsable du Laboratoire d'identification génétique de l'Institut de Médecine Légale de l'Université de Liège, Belgium

2 extracts each of them 2 times with the 2 kits   1 individual
11.1 [2] 101.61 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
11.2 103.17 ABI 3500xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
12.1 105.78 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
12.3 235.75 ABI 3500xl ESI Sivan Paz, Carla Oz, Israel Police DNA Database Re-extracted and re-amplified reported size is average of 235.78 bp and 235.71 bp 1 sample
12.3 [2] 94.03 ABI 3500 NGM SElect Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
13.1 109.91 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Crime scene sample 1 (first observation)
14.1 113.61 ABI 3500xl ESX17 (Promega) Christophe Frippiat, National Institute of Criminalistics and Criminology, Belgium re-amplified with kit ESI17Pro (size of 367.09)   1 individual
14.2 [2] 114.10 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)

13 reported variants as of 08/04/2016

 

Tri-Allelic Patterns

Allele Designation

 

Instrument

 

AMP KIT

 

Contributor

 

Verification/Conformation Method(s)

 

 

 

Notes

 

Frequency

 

 

0  reported tri-allelic patterns as of 08/04/2016

* Use the following abbreviations for amplification kit names:

§         CO: PE Applied Biosystems COfilerTM

§         PR: PE Applied Biosystems ProfilerTM

§         PS: PE Applied Biosystems Profiler PlusTM

§         ID: PE Applied Biosystems Indetifiler™

§         CV: Promega CTTVTM

§         CA: Promega CTTATM

§         PP: Promega PowerPlexTM

§         MP: Promega MonoPlexTM

  • Other: Please specify

 

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