Output for screening the Marshfield Set 12 (408 primers pairs)

http://research.marshfieldclinic.org/genetics/sets/combo.html

Note: results are best viewed in a Courier type font

 

 

 

Number of Hits = 1224

Number of Sequences = 816

Na+ = 0.085 Ct = 1.0

 

CATC015-F TAACCACCCATTCATCAACC versus GATA30E06-F TCTGTCTTAGATGGATGAATGG

Matches = 11

Score = 9

CCATTCATCNANC

est. tm =  11.5 oC

DeltaG @37 degrees =  -3.74 kcal/mole

 

                    3'-GGTAAGTAGGTAGATTCTGTCT-5'

                       |||||||||x|x|

             5'-TAACCACCCATTCATCAACC-3'

 

CATC015-F TAACCACCCATTCATCAACC versus GATA22F09N-F GTTCAAGCATGGGTGGATAG

Matches = 15

Score = 11

GTTNANGNATGGGTGGNTA

est. tm =  19.6 oC

DeltaG @37 degrees =  -4.85 kcal/mole

 

                    5'-GTTCAAGCATGGGTGGATAG-3'

                       |||x|x|x||||||||x||

                   3'-CCAACTACTTACCCACCAAT-5'

 

CATC015-F TAACCACCCATTCATCAACC versus GATA178G09-F ATGGGTGGATGTACAAATGG

Matches = 9

Score = 7

ATGGGTGGNTN

est. tm =  19.8 oC

DeltaG @37 degrees =  -6.21 kcal/mole

 

                    5'-ATGGGTGGATGTACAAATGG-3'

                       ||||||||x|x

           3'-CCAACTACTTACCCACCAAT-5'

 

CATC015-F TAACCACCCATTCATCAACC versus GATA118G10-F ACATACGGATGAATGGATGG

Matches = 12

Score = 7

CCANCCATTCATCNNNN

est. tm =  24.5 oC

DeltaG @37 degrees =  -6.07 kcal/mole

 

                    3'-GGTAGGTAAGTAGGCATACA-5'

                       |||x|||||||||xxxx

                 5'-TAACCACCCATTCATCAACC-3'

 

CATC015-F TAACCACCCATTCATCAACC versus GATA43H01-R ACGTGGTACAGGTAGATAAATGG

Matches = 11

Score = 9

CCATTNATCNACC

est. tm =  13.5 oC

DeltaG @37 degrees =  -4.25 kcal/mole

 

                    3'-GGTAAATAGATGGACATGGTGCA-5'

                       |||||x|||x|||

             5'-TAACCACCCATTCATCAACC-3'

 

GATA185H04N-R ATATTTCAATATTGTAACCAGTCC versus GATA27E01-F TTTATTGAGATATATTTGACATGCA

Matches = 10

Score = 7

NNTATTGANATAT

est. tm =  0.3 oC

DeltaG @37 degrees =  -1.71 kcal/mole

 

                        5'-TTTATTGAGATATATTTGACATGCA-3'

                           xx||||||x||||

             3'-CCTGACCAATGTTATAACTTTATA-5'

 

ATA47D07-F TCGAGGCTGCAGTGAACTAT versus AATA036-R ACTGACTGCAGCCTTGACCT

Matches = 13

Score = 9

NNTNACTGCAGCCTNGA

est. tm =  33.4 oC

DeltaG @37 degrees =  -8.43 kcal/mole

 

                    5'-ACTGACTGCAGCCTTGACCT-3'

                       xx|x||||||||||x||

                 3'-TATCAAGTGACGTCGGAGCT-5'

 

GATA129H04-F AGAGAACGGAACCAACAGAA versus GATA43C11-F TTCAAGTGGTTGGGTTCACT

Matches = 14

Score = 8

TTCNNNTGGTTNNGTTCNCT

est. tm =  12.7 oC

DeltaG @37 degrees =  -3.07 kcal/mole

 

                    5'-TTCAAGTGGTTGGGTTCACT-3'

                       |||xxx|||||xx||||x||

                    3'-AAGACAACCAAGGCAAGAGA-5'

 

GATA129H04-F AGAGAACGGAACCAACAGAA versus GATA43C11-F TTCAAGTGGTTGGGTTCACT

Matches = 14

Score = 8

AGNGAACNNAACCANNNGAA

est. tm =  12.7 oC

DeltaG @37 degrees =  -3.07 kcal/mole

 

                    3'-TCACTTGGGTTGGTGAACTT-5'

                       ||x||||xx|||||xxx|||

                    5'-AGAGAACGGAACCAACAGAA-3'

 

GATA26G09N-F CTCCAGAGAAGCAGAACCAG versus PAH-F GCCAGAACAACTGCTGGTTC

Matches = 7

Score = 7

GAACCAG

est. tm =  8.5 oC

DeltaG @37 degrees =  -4.45 kcal/mole

 

                    3'-CTTGGTCGTCAACAAGACCG-5'

                       |||||||

       5'-CTCCAGAGAAGCAGAACCAG-3'

 

GATA109-F TAGGCAAATAATAAAATTCTAACCA versus ATA25E07-F TAATTTTAATTTTCTCCAAATCTCC

Matches = 13

Score = 7

NAATTTTANTNTTNNCCNA

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                         5'-TAATTTTAATTTTCTCCAAATCTCC-3'

                            x|||||||x|x||xx||x|

                   3'-ACCAATCTTAAAATAATAAACGGAT-5'

 

GATA7C06-R TAGGATTTTACTATATTCCCCAGG versus GATA109-F TAGGCAAATAATAAAATTCTAACCA

Matches = 15

Score = 9

NNGGNANATANTAAAATNCTA

est. tm =  2.0 oC

DeltaG @37 degrees =  -0.35 kcal/mole

 

                        5'-TAGGCAAATAATAAAATTCTAACCA-3'

                           xx||x|x|||x||||||x|||

                     3'-GGACCCCTTATATCATTTTAGGAT-5'

 

GATA61E03-R GGTATTTTTTGCCCACATGA versus GATA109-F TAGGCAAATAATAAAATTCTAACCA

Matches = 11

Score = 7

TNGGCAAANAATANN

est. tm =  10.1 oC

DeltaG @37 degrees =  -3.58 kcal/mole

 

                    5'-TAGGCAAATAATAAAATTCTAACCA-3'

                       |x||||||x||||xx

               3'-AGTACACCCGTTTTTTATGG-5'

 

GATA109-F TAGGCAAATAATAAAATTCTAACCA versus GATA121A08N-F GTTAACAGACTATTACCTGCCTACC

Matches = 16

Score = 9

GTTANNANNNTATTANNTGCCTA

est. tm =  14.1 oC

DeltaG @37 degrees =  -2.27 kcal/mole

 

                         5'-GTTAACAGACTATTACCTGCCTACC-3'

                            ||||xx|xxx|||||xx||||||

                       3'-ACCAATCTTAAAATAATAAACGGAT-5'

 

GATA169E06-R TGGAATTCAGTTATTTGTTAATGC versus GATA109-F TAGGCAAATAATAAAATTCTAACCA

Matches = 14

Score = 7

TNNNCAAATAANNNAATTCNA

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                        5'-TAGGCAAATAATAAAATTCTAACCA-3'

                           |xxx|||||||xxx|||||x|

                     3'-CGTAATTGTTTATTGACTTAAGGT-5'

 

GATA124C08N-F TTCTAACAAGCTCCCAGGTG versus GATA5D08-R TATGACAGGCATCTGGGAGT

Matches = 9

Score = 7

NCTCCCAGNTG

est. tm =  17.4 oC

DeltaG @37 degrees =  -5.60 kcal/mole

 

                    3'-TGAGGGTCTACGGACAGTAT-5'

                       x|||||||x||

           5'-TTCTAACAAGCTCCCAGGTG-3'

 

GATA124C08N-F TTCTAACAAGCTCCCAGGTG versus GATA193A07-R AGCCTGTGGCATCTTGTTAG

Matches = 13

Score = 8

CTAACAAGNTNCCANNNG

est. tm =  8.8 oC

DeltaG @37 degrees =  -2.40 kcal/mole

 

                    3'-GATTGTTCTACGGTGTCCGA-5'

                       ||||||||x|x|||xxx|

                  5'-TTCTAACAAGCTCCCAGGTG-3'

 

GATA124C08N-F TTCTAACAAGCTCCCAGGTG versus GATA176C01-F TCTCACTACACCACCTGGGT

Matches = 8

Score = 7

NCCCAGGTG

est. tm =  25.8 oC

DeltaG @37 degrees =  -7.24 kcal/mole

 

                    3'-TGGGTCCACCACATCACTCT-5'

                       x||||||||

         5'-TTCTAACAAGCTCCCAGGTG-3'

 

GATA124C08N-F TTCTAACAAGCTCCCAGGTG versus ATA7D07-R ACGGGAGCTTTTGAGAAGTT

Matches = 13

Score = 9

NNGGGAGCTTNTNAGAA

est. tm =  27.8 oC

DeltaG @37 degrees =  -7.00 kcal/mole

 

                    5'-ACGGGAGCTTTTGAGAAGTT-3'

                       xx||||||||x|x||||

                 3'-GTGGACCCTCGAACAATCTT-5'

 

ATA25E07-F TAATTTTAATTTTCTCCAAATCTCC versus ATA50C05-F TTTGGAAAGAATCTGTATTATCTTT

Matches = 14

Score = 8

TTTGGANANAATNNNNATTA

est. tm =  4.0 oC

DeltaG @37 degrees =  -0.92 kcal/mole

 

                         5'-TTTGGAAAGAATCTGTATTATCTTT-3'

                            ||||||x|x|||xxxx||||

                    3'-CCTCTAAACCTCTTTTAATTTTAAT-5'

 

ATA21F01-R ATGACCAAAATGAAAATCACTG versus ATA25E07-F TAATTTTAATTTTCTCCAAATCTCC

Matches = 13

Score = 7

TNATTTTNATTTTNNNCAN

est. tm =  1.8 oC

DeltaG @37 degrees =  -0.92 kcal/mole

 

                      5'-TAATTTTAATTTTCTCCAAATCTCC-3'

                         |x|||||x|||||xxx||x

                   3'-GTCACTAAAAGTAAAACCAGTA-5'

 

GATA64G07-R AGACTTGGACTGAATTACACTGC versus ATA25E07-F TAATTTTAATTTTCTCCAAATCTCC

Matches = 15

Score = 7

NNANTNTAATTNNNTCCAANTCT

est. tm =  5.3 oC

DeltaG @37 degrees =  -1.02 kcal/mole

 

                       5'-TAATTTTAATTTTCTCCAAATCTCC-3'

                          xx|x|x|||||xxx|||||x|||

                       3'-CGTCACATTAAGTCAGGTTCAGA-5'

 

AAAAC001-R CCTTTGGAGAAAACTGAACTCG versus ATA25E07-F TAATTTTAATTTTCTCCAAATCTCC

Matches = 15

Score = 8

NNANTTNANTTTTCTCCAAANN

est. tm =  21.0 oC

DeltaG @37 degrees =  -4.70 kcal/mole

 

                      5'-TAATTTTAATTTTCTCCAAATCTCC-3'

                         xx|x||x|x|||||||||||xx

                      3'-GCTCAAGTCAAAAGAGGTTTCC-5'

 

ATA7D07-R ACGGGAGCTTTTGAGAAGTT versus ATA25E07-F TAATTTTAATTTTCTCCAAATCTCC

Matches = 13

Score = 9

GGAGNTTTNGAGAANNT

est. tm =  10.4 oC

DeltaG @37 degrees =  -3.01 kcal/mole

 

                    3'-CCTCTAAACCTCTTTTAATTTTAAT-5'

                       ||||x|||x|||||xx|

                 5'-ACGGGAGCTTTTGAGAAGTT-3'

 

ATA25E07-F TAATTTTAATTTTCTCCAAATCTCC versus GATA165B12-R TTAAAATCATTTTCACTGTGTATGC

Matches = 8

Score = 7

TTAAAATNA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.54 kcal/mole

 

                         5'-TTAAAATCATTTTCACTGTGTATGC-3'

                            |||||||x|

         3'-CCTCTAAACCTCTTTTAATTTTAAT-5'

 

GATA12A07N-F AGCACATAGCAGGCACTAGC versus ATA26B08-F ACTGGTATGTCCTAACCCCC

Matches = 10

Score = 7

NCTGNTATGTNCT

est. tm =  0.9 oC

DeltaG @37 degrees =  -2.53 kcal/mole

 

                    5'-ACTGGTATGTCCTAACCCCC-3'

                       x|||x|||||x||

             3'-CGATCACGGACGATACACGA-5'

 

GATA71C09-F GAGTTGCGTTACTTGATTTCTT versus GGAA5F09-F GCCATCAAGAAAACTAGTACTGC

Matches = 13

Score = 9

GCNNTACTNGNTTTCTT

est. tm =  10.1 oC

DeltaG @37 degrees =  -2.83 kcal/mole

 

                      3'-CGTCATGATCAAAAGAACTACCG-5'

                         ||xx||||x|x||||||

                 5'-GAGTTGCGTTACTTGATTTCTT-3'

 

TATC028-F CTGCATGACCTTGGGAAAAT versus ATT001-F GCCATGCAGGAAGGAGTAGA

Matches = 8

Score = 7

GNCATGCAG

est. tm =  6.3 oC

DeltaG @37 degrees =  -3.90 kcal/mole

 

                    5'-GCCATGCAGGAAGGAGTAGA-3'

                       |x|||||||

         3'-TAAAAGGGTTCCAGTACGTC-5'

 

TATC028-F CTGCATGACCTTGGGAAAAT versus GATA7C06-R TAGGATTTTACTATATTCCCCAGG

Matches = 10

Score = 8

CCTNGGGAANAT

est. tm =  6.9 oC

DeltaG @37 degrees =  -3.80 kcal/mole

 

                    3'-GGACCCCTTATATCATTTTAGGAT-5'

                       |||x|||||x||

            5'-CTGCATGACCTTGGGAAAAT-3'

 

GATA7C01-F TGCAGATCTTGGGACTTCTC versus GATA124F08-R GAGAAAGAAGCCCCAAGAAC

Matches = 13

Score = 10

GNTCTTGGGNCTTCTN

est. tm =  29.3 oC

DeltaG @37 degrees =  -7.36 kcal/mole

 

                    3'-CAAGAACCCCGAAGAAAGAG-5'

                       |x|||||||x|||||x

                5'-TGCAGATCTTGGGACTTCTC-3'

 

GATA7C01-F TGCAGATCTTGGGACTTCTC versus ATA26B08-R GATCTGCAGTTGGATTCTGG

Matches = 8

Score = 8

GATCTGCA

est. tm =  17.8 oC

DeltaG @37 degrees =  -5.69 kcal/mole

 

                    5'-GATCTGCAGTTGGATTCTGG-3'

                       ||||||||

        3'-CTCTTCAGGGTTCTAGACGT-5'

 

GATA7C01-F TGCAGATCTTGGGACTTCTC versus GATA14E09-R GTCATAATATCTGCCAATGATTG

Matches = 11

Score = 7

GTCNNAANATCTGCN

est. tm =  15.5 oC

DeltaG @37 degrees =  -4.56 kcal/mole

 

                    5'-GTCATAATATCTGCCAATGATTG-3'

                       |||xx||x||||||x

               3'-CTCTTCAGGGTTCTAGACGT-5'

 

GATA124F08-F TATCTAGCTGGCTGGCTAGC versus GGAA22G10N-F AGTCAGCTTGATTGATCCAG

Matches = 10

Score = 7

AGNCAGCTNGATN

est. tm =  10.2 oC

DeltaG @37 degrees =  -4.25 kcal/mole

 

                    5'-AGTCAGCTTGATTGATCCAG-3'

                       ||x|||||x|||x

             3'-CGATCGGTCGGTCGATCTAT-5'

 

GATA124F08-F TATCTAGCTGGCTGGCTAGC versus GATA27C08N-F GGCAGGCAGGTAGATAGACA

Matches = 13

Score = 10

GNCAGNCAGNTAGATA

est. tm =  7.8 oC

DeltaG @37 degrees =  -2.46 kcal/mole

 

                    5'-GGCAGGCAGGTAGATAGACA-3'

                       |x|||x|||x||||||

                3'-CGATCGGTCGGTCGATCTAT-5'

 

GATA11E02N-F TTGCCTTGTTAACATGTACAGG versus ATA29C07-F TTTCTCACCTTTAAATGTCATCA

Matches = 12

Score = 8

TGNTNACATNTANAGG

est. tm =  less than zero

DeltaG @37 degrees =  -0.85 kcal/mole

 

                      3'-ACTACTGTAAATTTCCACTCTTT-5'

                         ||x|x||||x||x|||

                5'-TTGCCTTGTTAACATGTACAGG-3'

 

GATA32F05-R AAGGTCAGAATCCCTCATCC versus ATA29C07-F TTTCTCACCTTTAAATGTCATCA

Matches = 9

Score = 7

NTTCTNACCTT

est. tm =  9.8 oC

DeltaG @37 degrees =  -4.28 kcal/mole

 

                    5'-TTTCTCACCTTTAAATGTCATCA-3'

                       x||||x|||||

           3'-CCTACTCCCTAAGACTGGAA-5'

 

GATA22D12-F TGCTATCAAATGACAAAGTTAGC versus GATA187D09N-F CTTAGTCTTTTCTTACCAAAACAGC

Matches = 13

Score = 8

CTTNGTCNTTTNNTANCA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.09 kcal/mole

 

                       5'-CTTAGTCTTTTCTTACCAAAACAGC-3'

                          |||x|||x|||xx||x||

                  3'-CGATTGAAACAGTAAACTATCGT-5'

 

GATA72G09N-R TTGGTGATTTAATTCCAGGC versus GATA22D12-F TGCTATCAAATGACAAAGTTAGC

Matches = 12

Score = 7

TGNNATNAAATNACNAA

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                    5'-TGCTATCAAATGACAAAGTTAGC-3'

                       ||xx||x||||x||x||

                 3'-CGGACCTTAATTTAGTGGTT-5'

 

AAT243-F AATCGCTTGAACCCAGGAG versus TTTA049-F TCCATGCGATTTATCCAAGA

Matches = 9

Score = 7

TNCANGCGATT

est. tm =  0.4 oC

DeltaG @37 degrees =  -3.11 kcal/mole

 

                   5'-TCCATGCGATTTATCCAAGA-3'

                      |x||x||||||

           3'-GAGGACCCAAGTTCGCTAA-5'

 

GGAA20G10-F TGGAATCGTTTTACCTCTGC versus GATA168F06-F ACAATTCCAGAAACTTCCCC

Matches = 8

Score = 7

ACNATTCCA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.95 kcal/mole

 

                    5'-ACAATTCCAGAAACTTCCCC-3'

                       ||x||||||

         3'-CGTCTCCATTTTGCTAAGGT-5'

 

GGAA20G10-F TGGAATCGTTTTACCTCTGC versus UT7136-F AGGAGGCAGAGGTTACAGTA

Matches = 11

Score = 7

TNNTNTNACCTCTGC

est. tm =  4.8 oC

DeltaG @37 degrees =  -3.04 kcal/mole

 

                    3'-ATGACATTGGAGACGGAGGA-5'

                       |xx|x|x||||||||

               5'-TGGAATCGTTTTACCTCTGC-3'

 

GATA8F07-F CAGAAGCGTGGCAGTTTATT versus GATA89A11-F GCTTCTGAAAGCTTCTAGTTTACC

Matches = 7

Score = 7

GCTTCTG

est. tm =  10.4 oC

DeltaG @37 degrees =  -4.69 kcal/mole

 

                    5'-GCTTCTGAAAGCTTCTAGTTTACC-3'

                       |||||||

       3'-TTATTTGACGGTGCGAAGAC-5'

 

ATA47C04N-F TCTCTCACACAAAAAAACCC versus GATA164B08N-R TGGGGTATCCTGTGTCAGAGC

Matches = 14

Score = 8

NCTCTNACACANNANANCCC

est. tm =  16.6 oC

DeltaG @37 degrees =  -4.37 kcal/mole

 

                    3'-CGAGACTGTGTCCTATGGGGT-5'

                       x||||x|||||xx|x|x|||

                    5'-TCTCTCACACAAAAAAACCC-3'

 

ATA47C04N-F TCTCTCACACAAAAAAACCC versus AGAT113Z-R CGCTACCACGGGTGTTTTTA

Matches = 9

Score = 7

NAAAAANACCC

est. tm =  10.9 oC

DeltaG @37 degrees =  -4.30 kcal/mole

 

                    3'-ATTTTTGTGGGCACCATCGC-5'

                       x|||||x||||

           5'-TCTCTCACACAAAAAAACCC-3'

 

GATA66D01-F TAATTCAAATCTTTCCTGTCAGG versus GATA66D01-F TAATTCAAATCTTTCCTGTCAGG

Matches = 8

Score = 7

CCTGNCAGG

est. tm =  7.3 oC

DeltaG @37 degrees =  -4.28 kcal/mole

 

                       3'-GGACTGTCCTTTCTAAACTTAAT-5'

                          ||||x||||

         5'-TAATTCAAATCTTTCCTGTCAGG-3'

 

ATT001-F GCCATGCAGGAAGGAGTAGA versus GATA66D01-F TAATTCAAATCTTTCCTGTCAGG

Matches = 13

Score = 7

CCNNNCAGGAANGANTNGA

est. tm =  1.5 oC

DeltaG @37 degrees =  -1.62 kcal/mole

 

                    3'-GGACTGTCCTTTCTAAACTTAAT-5'

                       ||xxx||||||x||x|x||

                   5'-GCCATGCAGGAAGGAGTAGA-3'

 

GATA66D01-F TAATTCAAATCTTTCCTGTCAGG versus GATA66D01-R TAATTCAAATCTTTCCTGTCAGG

Matches = 8

Score = 7

CCTGNCAGG

est. tm =  7.3 oC

DeltaG @37 degrees =  -4.28 kcal/mole

 

                       3'-GGACTGTCCTTTCTAAACTTAAT-5'

                          ||||x||||

         5'-TAATTCAAATCTTTCCTGTCAGG-3'

 

GATA88G05-F ACATGTCGATCTCAGCGTTC versus ATGT006Z-F AAGGCTGAGGTAGGCTTATTTTT

Matches = 13

Score = 7

AANGCTGAGNTNGNCNTNT

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                    5'-AAGGCTGAGGTAGGCTTATTTTT-3'

                       ||x||||||x|x|x|x|x|

                   3'-CTTGCGACTCTAGCTGTACA-5'

 

GATA4E11-F TGAGCGGAATGAAAAGAGAG versus GATA31F01-F TTGGTACTTAATAAACCCTCTTTT

Matches = 8

Score = 7

AAAAGAGNG

est. tm =  less than zero

DeltaG @37 degrees =  -2.00 kcal/mole

 

                    3'-TTTTCTCCCAAATAATTCATGGTT-5'

                       |||||||x|

         5'-TGAGCGGAATGAAAAGAGAG-3'

 

GATA4D07-F AGGCCTCTCTTCGAATGATT versus GGAA20G10-R TATAGGTCAACGATAACTCATTCG

Matches = 8

Score = 7

CGAATGANT

est. tm =  1.6 oC

DeltaG @37 degrees =  -3.21 kcal/mole

 

                    3'-GCTTACTCAATAGCAACTGGATAT-5'

                       |||||||x|

         5'-AGGCCTCTCTTCGAATGATT-3'

 

GATA4D07-F AGGCCTCTCTTCGAATGATT versus GATA43H03N-R ATTTGAACAGAGGCATGTAC

Matches = 13

Score = 10

ATTNGAANAGAGGCNT

est. tm =  15.6 oC

DeltaG @37 degrees =  -4.41 kcal/mole

 

                    5'-ATTTGAACAGAGGCATGTAC-3'

                       |||x|||x||||||x|

                3'-TTAGTAAGCTTCTCTCCGGA-5'

 

GATA62F03N-R AGCCAAAATGTTATGCAAG versus ATA27H09-F CCAGGGACATTGCTTAACAT

Matches = 10

Score = 8

ATGTTANGCAAN

est. tm =  18.1 oC

DeltaG @37 degrees =  -5.30 kcal/mole

 

                   3'-TACAATTCGTTACAGGGACC-5'

                      ||||||x||||x

            5'-AGCCAAAATGTTATGCAAG-3'

 

ATA27H09-F CCAGGGACATTGCTTAACAT versus GATA81D12-R TAATGTACCTGGTACCAAAAACA

Matches = 10

Score = 8

NAATGTNCCTGG

est. tm =  21.6 oC

DeltaG @37 degrees =  -6.06 kcal/mole

 

                    5'-TAATGTACCTGGTACCAAAAACA-3'

                       x|||||x|||||

            3'-TACAATTCGTTACAGGGACC-5'

 

ATA27H09-F CCAGGGACATTGCTTAACAT versus GATA4A10-R TCCCTGGGTGAAAATGAGTA

Matches = 7

Score = 7

TCCCTGG

est. tm =  13.4 oC

DeltaG @37 degrees =  -5.58 kcal/mole

 

                    5'-TCCCTGGGTGAAAATGAGTA-3'

                       |||||||

       3'-TACAATTCGTTACAGGGACC-5'

 

GGAA30H04-F ATTCTTCTCCCAAGAATGCC versus GATA65C03N-F CTCACTGTCTGGATTTCTTGG

Matches = 9

Score = 7

CCAAGAANNCC

est. tm =  9.2 oC

DeltaG @37 degrees =  -3.91 kcal/mole

 

                    3'-GGTTCTTTAGGTCTGTCACTC-5'

                       |||||||xx||

           5'-ATTCTTCTCCCAAGAATGCC-3'

 

AAAT072-R ACTCCACCCAGGAAGTCCA versus GATA65C03N-F CTCACTGTCTGGATTTCTTGG

Matches = 10

Score = 8

CCANGAANTCCA

est. tm =  9.2 oC

DeltaG @37 degrees =  -4.05 kcal/mole

 

                   3'-GGTTCTTTAGGTCTGTCACTC-5'

                      |||x|||x||||

            5'-ACTCCACCCAGGAAGTCCA-3'

 

GATA65C03N-F CTCACTGTCTGGATTTCTTGG versus GATA10G07-F AATCCAGTAAATGAAATAGTCATCA

Matches = 11

Score = 7

AATCCAGNNANTGAN

est. tm =  12.3 oC

DeltaG @37 degrees =  -4.10 kcal/mole

 

                     5'-AATCCAGTAAATGAAATAGTCATCA-3'

                        |||||||xx|x|||x

               3'-GGTTCTTTAGGTCTGTCACTC-5'

 

UT7544-F ACTCCAGTCTGTGTGACAG versus GATA65C03N-F CTCACTGTCTGGATTTCTTGG

Matches = 11

Score = 7

CNCACNGNCTGGANT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.98 kcal/mole

 

                   5'-CTCACTGTCTGGATTTCTTGG-3'

                      |x|||x|x|||||x|

               3'-GACAGTGTGTCTGACCTCA-5'

 

GATA7G10-R GCCCAAAAAGACAGACAGAA versus GATA65C03N-F CTCACTGTCTGGATTTCTTGG

Matches = 13

Score = 7

CCNANAAANNCAGACAGNN

est. tm =  14.4 oC

DeltaG @37 degrees =  -3.80 kcal/mole

 

                    3'-GGTTCTTTAGGTCTGTCACTC-5'

                       ||x|x|||xx|||||||xx

                   5'-GCCCAAAAAGACAGACAGAA-3'

 

GATA30E06-F TCTGTCTTAGATGGATGAATGG versus GATA13-R AGACAGAAATATAGATGAGAATGCA

Matches = 7

Score = 7

AGACAGA

est. tm =  4.0 oC

DeltaG @37 degrees =  -4.09 kcal/mole

 

                      5'-AGACAGAAATATAGATGAGAATGCA-3'

                         |||||||

       3'-GGTAAGTAGGTAGATTCTGTCT-5'

 

GATA30E06-F TCTGTCTTAGATGGATGAATGG versus GATA129D11N-R TAGTACTCTACCATCCATCTATCCC

Matches = 11

Score = 7

NNGATNGATGNATGG

est. tm =  8.7 oC

DeltaG @37 degrees =  -3.21 kcal/mole

 

                      3'-CCCTATCTACCTACCATCTCATGAT-5'

                         xx|||x||||x||||

               5'-TCTGTCTTAGATGGATGAATGG-3'

 

GGAT3F08-R TATCCATCCATCCATCAACC versus GATA30E06-F TCTGTCTTAGATGGATGAATGG

Matches = 10

Score = 7

CCATNCATCNANC

est. tm =  less than zero

DeltaG @37 degrees =  -1.01 kcal/mole

 

                    3'-GGTAAGTAGGTAGATTCTGTCT-5'

                       ||||x||||x|x|

             5'-TATCCATCCATCCATCAACC-3'

 

GGAT3F08-R TATCCATCCATCCATCAACC versus GATA30E06-F TCTGTCTTAGATGGATGAATGG

Matches = 13

Score = 9

CCATNCATCCATCNANN

est. tm =  25.4 oC

DeltaG @37 degrees =  -6.33 kcal/mole

 

                    3'-GGTAAGTAGGTAGATTCTGTCT-5'

                       ||||x||||||||x|xx

                 5'-TATCCATCCATCCATCAACC-3'

 

GATA23A02-F AGAAACAGAACGGATAGGGT versus GATA64F08-R TGACACTATCAGCTCTCTGGC

Matches = 13

Score = 7

GNNANAGANCNGATAGNGT

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                    3'-CGGTCTCTCGACTATCACAGT-5'

                       |xx|x|||x|x|||||x||

                   5'-AGAAACAGAACGGATAGGGT-3'

 

GATA23A02-F AGAAACAGAACGGATAGGGT versus GATA29A05-F TTTTGTTTCTTGATCTGGGC

Matches = 9

Score = 8

TTNTGTTTCT

est. tm =  4.3 oC

DeltaG @37 degrees =  -3.29 kcal/mole

 

                    5'-TTTTGTTTCTTGATCTGGGC-3'

                       ||x|||||||

          3'-TGGGATAGGCAAGACAAAGA-5'

 

ATA78D02N-F GGCAAGAGTTTTCCCATTTT versus AGAT021-F GAAACTCCAAAGATGGCAAAA

Matches = 10

Score = 8

TTTTNCCATNTT

est. tm =  less than zero

DeltaG @37 degrees =  -1.99 kcal/mole

 

                    3'-AAAACGGTAGAAACCTCAAAG-5'

                       ||||x||||x||

            5'-GGCAAGAGTTTTCCCATTTT-3'

 

GATA175D03-F TGGAGTCTCTGGGTGAAGAG versus AGAT021-F GAAACTCCAAAGATGGCAAAA

Matches = 8

Score = 7

GANACTCCA

est. tm =  3.3 oC

DeltaG @37 degrees =  -3.73 kcal/mole

 

                    5'-GAAACTCCAAAGATGGCAAAA-3'

                       ||x||||||

         3'-GAGAAGTGGGTCTCTGAGGT-5'

 

GATA4G12-R GCCATCTGTACTGTTCCCAG versus AGAT021-F GAAACTCCAAAGATGGCAAAA

Matches = 12

Score = 7

GNAACNNNANAGATGGC

est. tm =  7.7 oC

DeltaG @37 degrees =  -2.74 kcal/mole

 

                    5'-GAAACTCCAAAGATGGCAAAA-3'

                       |x|||xxx|x|||||||

                 3'-GACCCTTGTCATGTCTACCG-5'

 

GATA164B08N-F CTGTGATCACACCACTGCAG versus GATA164B08N-F CTGTGATCACACCACTGCAG

Matches = 10

Score = 8

NTGTGATCACAN

est. tm =  33.3 oC

DeltaG @37 degrees =  -8.47 kcal/mole

 

                    5'-CTGTGATCACACCACTGCAG-3'

                       x||||||||||x

            3'-GACGTCACCACACTAGTGTC-5'

 

GATA164B08N-F CTGTGATCACACCACTGCAG versus ATA34G06-F GATCACAGGTTAATGGGTGC

Matches = 8

Score = 8

GATCACAG

est. tm =  14.7 oC

DeltaG @37 degrees =  -4.97 kcal/mole

 

                    5'-GATCACAGGTTAATGGGTGC-3'

                       ||||||||

        3'-GACGTCACCACACTAGTGTC-5'

 

GATA164B08N-F CTGTGATCACACCACTGCAG versus UT5029-R AAATCATCAGTGGAGTTAGCA

Matches = 12

Score = 7

TGNTNACNCCACTGNNG

est. tm =  less than zero

DeltaG @37 degrees =  -1.62 kcal/mole

 

                    3'-ACGATTGAGGTGACTACTAAA-5'

                       ||x|x||x||||||xx|

                 5'-CTGTGATCACACCACTGCAG-3'

 

GATA164B08N-F CTGTGATCACACCACTGCAG versus GATA23B01N-F GTGTAAGCAGTGTGCAGTGG

Matches = 8

Score = 7

CCACTGCAN

est. tm =  28.4 oC

DeltaG @37 degrees =  -7.68 kcal/mole

 

                    3'-GGTGACGTGTGACGAATGTG-5'

                       ||||||||x

         5'-CTGTGATCACACCACTGCAG-3'

 

GATA164B08N-F CTGTGATCACACCACTGCAG versus GATA43C11-R TGGGTCTAGTCCAGTGGTGT

Matches = 10

Score = 8

ACACCACTGNAN

est. tm =  26.1 oC

DeltaG @37 degrees =  -7.05 kcal/mole

 

                    3'-TGTGGTGACCTGATCTGGGT-5'

                       |||||||||x|x

            5'-CTGTGATCACACCACTGCAG-3'

 

GATA73D01-F CATCTTTCTTTTCCTGTTCCC versus GATA65C03N-R TAAGAACAAGTAAAAAAAGCTGG

Matches = 15

Score = 9

NNGAACANGNAAANAAAGNTG

est. tm =  12.9 oC

DeltaG @37 degrees =  -2.77 kcal/mole

 

                     5'-TAAGAACAAGTAAAAAAAGCTGG-3'

                         xx|||||x|x|||x||||x||

                      3'-CCCTTGTCCTTTTCTTTCTAC-5'

 

GGAA2A03-F CCTGGGCAACAGAATAAGAT versus GATA73D01-F CATCTTTCTTTTCCTGTTCCC

Matches = 12

Score = 8

GGNAACAGNANAAGAN

est. tm =  12.7 oC

DeltaG @37 degrees =  -3.99 kcal/mole

 

                    3'-CCCTTGTCCTTTTCTTTCTAC-5'

                       ||x|||||x|x||||x

                5'-CCTGGGCAACAGAATAAGAT-3'

 

GATA73D01-F CATCTTTCTTTTCCTGTTCCC versus GATA22G05-R AAAACAAATATGGCTCTATCTATCG

Matches = 10

Score = 8

AAAANAAANATG

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.23 kcal/mole

 

                     5'-AAAACAAATATGGCTCTATCTATCG-3'

                        ||||x|||x|||

            3'-CCCTTGTCCTTTTCTTTCTAC-5'

 

GATA73D01-F CATCTTTCTTTTCCTGTTCCC versus GATA46A12-R AAAGAGTAAACTCAATGAAAGATGC

Matches = 14

Score = 7

GNGNANANNNAANGAAAGATG

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                     5'-AAAGAGTAAACTCAATGAAAGATGC-3'

                           |x|x|x|xxx||x||||||||

                        3'-CCCTTGTCCTTTTCTTTCTAC-5'

 

GATA6F06-F ACCACATGAGCCAATTCTGT versus ATTT019-R TTCATGGCCACAGAATTCAC

Matches = 9

Score = 7

GNNAATTCTGT

est. tm =  1.4 oC

DeltaG @37 degrees =  -2.52 kcal/mole

 

                    3'-CACTTAAGACACCGGTACTT-5'

                       |xx||||||||

           5'-ACCACATGAGCCAATTCTGT-3'

 

GATA6F06-F ACCACATGAGCCAATTCTGT versus GATA11B12-R GGTGGAAATGCCTCATGTAG

Matches = 13

Score = 7

CNACATGAGNCANTTCNNN

est. tm =  12.7 oC

DeltaG @37 degrees =  -3.32 kcal/mole

 

                    3'-GATGTACTCCGTAAAGGTGG-5'

                       |x|||||||x||x|||xxx

                   5'-ACCACATGAGCCAATTCTGT-3'

 

GATA6F06-F ACCACATGAGCCAATTCTGT versus GGAA4B09N-F ACAGAATGAGACCATGTGTCA

Matches = 14

Score = 8

ACAGAATNNGNNCATGTGNN

est. tm =  23.3 oC

DeltaG @37 degrees =  -5.45 kcal/mole

 

                    5'-ACAGAATGAGACCATGTGTCA-3'

                       |||||||xx|xx||||||xx

                    3'-TGTCTTAACCGAGTACACCA-5'

 

GATA6F06-F ACCACATGAGCCAATTCTGT versus ATA19H08-F TTCCAGAGTATTTGCTTAAGAGG

Matches = 13

Score = 7

CCNCNTNAGCNAATNCTNT

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                    3'-GGAGAATTCGTTTATGAGACCTT-5'

                       ||x|x|x|||x|||x||x|

                   5'-ACCACATGAGCCAATTCTGT-3'

 

GATA6F06-F ACCACATGAGCCAATTCTGT versus GATA64H04-F GATGATAGAGATGGCACATGA

Matches = 12

Score = 7

NCATGNGCCANNTCTNT

est. tm =  12.8 oC

DeltaG @37 degrees =  -4.10 kcal/mole

 

                    3'-AGTACACGGTAGAGATAGTAG-5'

                       x||||x||||xx|||x|

                 5'-ACCACATGAGCCAATTCTGT-3'

 

ATA22G07-R TGCTCTGTCTATGGAGTAGCC versus GATA7F05-F TCCAGAGAAACAGAATCAATAGG

Matches = 11

Score = 7

TCCANAGANANAGNA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.61 kcal/mole

 

                     5'-TCCAGAGAAACAGAATCAATAGG-3'

                        ||||x|||x|x||x|

               3'-CCGATGAGGTATCTGTCTCGT-5'

 

GATA31D10-F GTGGGACCTTGTGATTGTGT versus GATA7F05-F TCCAGAGAAACAGAATCAATAGG

Matches = 11

Score = 8

CCTNNTGATTNTGT

est. tm =  10.0 oC

DeltaG @37 degrees =  -3.55 kcal/mole

 

                    3'-GGATAACTAAGACAAAGAGACCT-5'

                       |||xx|||||x|||

              5'-GTGGGACCTTGTGATTGTGT-3'

 

GATA84B12-F ACCAAATGAGACAGTGGCAT versus ATA5A04N-F TGCCACTGACTTCATTGCTA

Matches = 14

Score = 9

TGCCACTGNCTNNNTTGNT

est. tm =  22.3 oC

DeltaG @37 degrees =  -5.54 kcal/mole

 

                    5'-TGCCACTGACTTCATTGCTA-3'

                       ||||||||x||xxx|||x|

                   3'-TACGGTGACAGAGTAAACCA-5'

 

GATA84B12-F ACCAAATGAGACAGTGGCAT versus ATTT019-F GCTGCCACTTTCCCTTTTAC

Matches = 13

Score = 7

NNAAANGNGANAGTGGCAN

est. tm =  22.2 oC

DeltaG @37 degrees =  -5.72 kcal/mole

 

                    3'-CATTTTCCCTTTCACCGTCG-5'

                       xx|||x|x||x|||||||x

                   5'-ACCAAATGAGACAGTGGCAT-3'

 

GATA84B12-F ACCAAATGAGACAGTGGCAT versus GATA12F04-R TATAGGCACATGCCACTGTG

Matches = 10

Score = 9

NACAGTGGCAT

est. tm =  35.3 oC

DeltaG @37 degrees =  -8.98 kcal/mole

 

                    3'-GTGTCACCGTACACGGATAT-5'

                       x||||||||||

           5'-ACCAAATGAGACAGTGGCAT-3'

 

GATA84B12-F ACCAAATGAGACAGTGGCAT versus ATA28C05-F TGTCTGCTAATGAATGATTTGG

Matches = 13

Score = 7

CCAAATNANNCANTNGCAN

est. tm =  9.1 oC

DeltaG @37 degrees =  -3.03 kcal/mole

 

                    3'-GGTTTAGTAAGTAATCGTCTGT-5'

                       ||||||x|xx||x|x|||x

                   5'-ACCAAATGAGACAGTGGCAT-3'

 

ATA34G06-F GATCACAGGTTAATGGGTGC versus TTAT023z-R CCTGTAATCCCAGCTGCTCT

Matches = 8

Score = 7

CCTGTNATC

est. tm =  2.1 oC

DeltaG @37 degrees =  -3.35 kcal/mole

 

                    5'-CCTGTAATCCCAGCTGCTCT-3'

                       |||||x|||

         3'-CGTGGGTAATTGGACACTAG-5'

 

ATA34G06-F GATCACAGGTTAATGGGTGC versus GGAT3F08-R TATCCATCCATCCATCAACC

Matches = 10

Score = 7

GGTTNATGGNTGN

est. tm =  12.0 oC

DeltaG @37 degrees =  -4.49 kcal/mole

 

                    3'-CCAACTACCTACCTACCTAT-5'

                       ||||x||||x||x

             5'-GATCACAGGTTAATGGGTGC-3'

 

GATA3C02-F TTTAAGCGGAAGGAAGTGTG versus ATA29C03-F GACTCTGCTAAGCACATTTCC

Matches = 8

Score = 7

GGAANTGTG

est. tm =  4.6 oC

DeltaG @37 degrees =  -3.66 kcal/mole

 

                    3'-CCTTTACACGAATCGTCTCAG-5'

                       ||||x||||

         5'-TTTAAGCGGAAGGAAGTGTG-3'

 

GATA3C02-F TTTAAGCGGAAGGAAGTGTG versus ATA47C04N-R AACATATCCTTCCCCTTCCA

Matches = 14

Score = 8

NACANNTCCTTCCNCTTNNA

est. tm =  19.2 oC

DeltaG @37 degrees =  -4.75 kcal/mole

 

                    5'-AACATATCCTTCCCCTTCCA-3'

                       x|||xx|||||||x|||xx|

                    3'-GTGTGAAGGAAGGCGAATTT-5'

 

GATA3C02-F TTTAAGCGGAAGGAAGTGTG versus ATA47C04N-R AACATATCCTTCCCCTTCCA

Matches = 14

Score = 8

TNNAAGNGGAAGGANNTGTN

est. tm =  20.8 oC

DeltaG @37 degrees =  -5.15 kcal/mole

 

                    3'-ACCTTCCCCTTCCTATACAA-5'

                       |xx|||x|||||||xx|||x

                    5'-TTTAAGCGGAAGGAAGTGTG-3'

 

GATA3C02-F TTTAAGCGGAAGGAAGTGTG versus GATA30D09N-F TCCAAGTAAAGGCCAGACTTCC

Matches = 8

Score = 7

GGAAGTNTG

est. tm =  3.3 oC

DeltaG @37 degrees =  -3.49 kcal/mole

 

                    3'-CCTTCAGACCGGAAATGAACCT-5'

                       ||||||x||

         5'-TTTAAGCGGAAGGAAGTGTG-3'

 

GATA3C02-F TTTAAGCGGAAGGAAGTGTG versus GATA32B03-R AGGTATGGTGCCACACACTT

Matches = 7

Score = 7

AAGTGTG

est. tm =  6.1 oC

DeltaG @37 degrees =  -4.16 kcal/mole

 

                    3'-TTCACACACCGTGGTATGGA-5'

                       |||||||

       5'-TTTAAGCGGAAGGAAGTGTG-3'

 

GATA3H01-F AAACCGCTGTGCACTTTATC versus ATA27F01-F GAGTTTGAAAATAAAGTGTTCTGC

Matches = 11

Score = 7

GCNGNNCACTTTATN

est. tm =  10.9 oC

DeltaG @37 degrees =  -3.54 kcal/mole

 

                    3'-CGTCTTGTGAAATAAAAGTTTGAG-5'

                       ||x|xx||||||||x

               5'-AAACCGCTGTGCACTTTATC-3'

 

GATA3H01-F AAACCGCTGTGCACTTTATC versus ATA18A07-R TATATGAAGTGCTTAGAAAAAGTGC

Matches = 9

Score = 8

GCACTTTNTC

est. tm =  13.5 oC

DeltaG @37 degrees =  -4.63 kcal/mole

 

                    3'-CGTGAAAAAGATTCGTGAAGTATAT-5'

                       |||||||x||

          5'-AAACCGCTGTGCACTTTATC-3'

 

TTTA040-F GGGTGCAGTTTTTCTCTGCT versus GATA73D10N-F TCATGACAAAACAGAAAATAAGTG

Matches = 11

Score = 7

CANTTNTTNTCTGNT

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                    3'-GTGAATAAAAGACAAAACAGTACT-5'

                       ||x||x||x||||x|

               5'-GGGTGCAGTTTTTCTCTGCT-3'

 

TTTA040-F GGGTGCAGTTTTTCTCTGCT versus GATA8G10-F GGGACAGAGAAGCAGAGAAG

Matches = 9

Score = 8

NTTCTCTGCT

est. tm =  25.6 oC

DeltaG @37 degrees =  -6.95 kcal/mole

 

                    3'-GAAGAGACGAAGAGACAGGG-5'

                       x|||||||||

          5'-GGGTGCAGTTTTTCTCTGCT-3'

 

AAAT072-R ACTCCACCCAGGAAGTCCA versus TTTA040-F GGGTGCAGTTTTTCTCTGCT

Matches = 8

Score = 7

GGGTGNAGT

est. tm =  6.8 oC

DeltaG @37 degrees =  -4.38 kcal/mole

 

                   5'-GGGTGCAGTTTTTCTCTGCT-3'

                      |||||x|||

         3'-ACCTGAAGGACCCACCTCA-5'

 

GATA49C09N-F GCAACAGAGCAAGACTGTC versus TTTA040-F GGGTGCAGTTTTTCTCTGCT

Matches = 12

Score = 7

ANCAGAGNAANACTGNN

est. tm =  8.3 oC

DeltaG @37 degrees =  -3.15 kcal/mole

 

                   3'-TCGTCTCTTTTTGACGTGGG-5'

                      |x|||||x||x||||xx

                 5'-GCAACAGAGCAAGACTGTC-3'

 

TTTA040-F GGGTGCAGTTTTTCTCTGCT versus ATA18A07-R TATATGAAGTGCTTAGAAAAAGTGC

Matches = 12

Score = 8

GCANTTTTTCTNNGCN

est. tm =  23.2 oC

DeltaG @37 degrees =  -5.91 kcal/mole

 

                    3'-CGTGAAAAAGATTCGTGAAGTATAT-5'

                       |||x|||||||xx||x

                5'-GGGTGCAGTTTTTCTCTGCT-3'

 

ATA50C05-R GCATAATGCACATGCCTGTA versus ATA22E01-F GTACATTCTGCACACGTACCC

Matches = 9

Score = 7

GTNCATTNTGC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.66 kcal/mole

 

                    5'-GTACATTCTGCACACGTACCC-3'

                       ||x||||x|||

           3'-ATGTCCGTACACGTAATACG-5'

 

GATA22G12-F AAAGCTAGAAGGAGCTGGCT versus 4PTEL04-F CTAGCTTTGATTCTATTGACC

Matches = 8

Score = 8

CTAGCTTT

est. tm =  11.1 oC

DeltaG @37 degrees =  -4.47 kcal/mole

 

                    5'-CTAGCTTTGATTCTATTGACC-3'

                       ||||||||

        3'-TCGGTCGAGGAAGATCGAAA-5'

 

GGAA2A03-F CCTGGGCAACAGAATAAGAT versus 4PTEL04-F CTAGCTTTGATTCTATTGACC

Matches = 12

Score = 7

GGNCAANAGAATNANAN

est. tm =  less than zero

DeltaG @37 degrees =  -1.44 kcal/mole

 

                    3'-CCAGTTATCTTAGTTTCGATC-5'

                       ||x|||x|||||x|x|x

                 5'-CCTGGGCAACAGAATAAGAT-3'

 

ATT015-F AGCACCCATATTTGCAGGAA versus GATA50D10-F ATGCCTACAAAAACCAGTGC

Matches = 13

Score = 7

GCACNNNTNTTTGNAGGNA

est. tm =  4.1 oC

DeltaG @37 degrees =  -2.01 kcal/mole

 

                    3'-CGTGACCAAAAACATCCGTA-5'

                       ||||xxx|x||||x|||x|

                   5'-AGCACCCATATTTGCAGGAA-3'

 

ATT015-F AGCACCCATATTTGCAGGAA versus GATA86H01-F GGTGCTTGCTGTAAATATAATTG

Matches = 13

Score = 8

CANNNATATTTNCAGNAA

est. tm =  0.2 oC

DeltaG @37 degrees =  -0.53 kcal/mole

 

                    3'-GTTAATATAAATGTCGTTCGTGG-5'

                       ||xxx||||||x|||x||

                  5'-AGCACCCATATTTGCAGGAA-3'

 

ATT015-F AGCACCCATATTTGCAGGAA versus TATC043-F GCCATCCTCCTGCAAATAAG

Matches = 12

Score = 10

CNTATTTGCAGGAN

est. tm =  31.0 oC

DeltaG @37 degrees =  -7.77 kcal/mole

 

                    3'-GAATAAACGTCCTCCTACCG-5'

                       |x|||||||||||x

              5'-AGCACCCATATTTGCAGGAA-3'

 

UT1772-R GGGTGCTAGGTGTGCTCA versus ATT015-F AGCACCCATATTTGCAGGAA

Matches = 7

Score = 7

AGCACCC

est. tm =  17.4 oC

DeltaG @37 degrees =  -6.12 kcal/mole

 

                  5'-AGCACCCATATTTGCAGGAA-3'

                     |||||||

       3'-ACTCGTGTGGATCGTGGG-5'

 

ATA27C07-F CATGCACACCAAAACAAGAA versus GATA22D12-R TTTGATGAGCTTGAACTAGGG

Matches = 10

Score = 7

TTTGNTGNGCNTG

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.79 kcal/mole

 

                    5'-TTTGATGAGCTTGAACTAGGG-3'

                       ||||x||x||x||

             3'-AAGAACAAAACCACACGTAC-5'

 

GATA61E03-R GGTATTTTTTGCCCACATGA versus ATA21F01-F GGTTTAAAAATACATCATGTGGC

Matches = 8

Score = 7

NCCACATGA

est. tm =  18.4 oC

DeltaG @37 degrees =  -5.89 kcal/mole

 

                    3'-CGGTGTACTACATAAAAATTTGG-5'

                       x||||||||

         5'-GGTATTTTTTGCCCACATGA-3'

 

GATA189C06-R GGAAGCCAAATGTTGAATTG versus ATA21F01-F GGTTTAAAAATACATCATGTGGC

Matches = 12

Score = 8

GCCANATGNTGNATTN

est. tm =  15.3 oC

DeltaG @37 degrees =  -4.53 kcal/mole

 

                    3'-CGGTGTACTACATAAAAATTTGG-5'

                       ||||x|||x||x|||x

                5'-GGAAGCCAAATGTTGAATTG-3'

 

ATA21F01-F GGTTTAAAAATACATCATGTGGC versus ATA27D04N-F TAACTATGTCATCTAGCCAAATGA

Matches = 8

Score = 7

TCATNTGGC

est. tm =  6.7 oC

DeltaG @37 degrees =  -4.24 kcal/mole

 

                       3'-AGTAAACCGATCTACTGTATCAAT-5'

                          ||||x||||

         5'-GGTTTAAAAATACATCATGTGGC-3'

 

GATA64H04-F GATGATAGAGATGGCACATGA versus ATA21F01-F GGTTTAAAAATACATCATGTGGC

Matches = 8

Score = 7

GNCACATGA

est. tm =  0.1 oC

DeltaG @37 degrees =  -3.09 kcal/mole

 

                     3'-CGGTGTACTACATAAAAATTTGG-5'

                        |x|||||||

         5'-GATGATAGAGATGGCACATGA-3'

 

ATA7D07-F ATCCCACAGGATGCCTATTT versus ATA21F01-F GGTTTAAAAATACATCATGTGGC

Matches = 13

Score = 8

NCCACANGATGNNTNTTT

est. tm =  13.7 oC

DeltaG @37 degrees =  -3.78 kcal/mole

 

                    3'-CGGTGTACTACATAAAAATTTGG-5'

                       x|||||x||||xx|x|||

                  5'-ATCCCACAGGATGCCTATTT-3'

 

GATA24H01N-F CAGGGAGACAGAACCAACTG versus GATA73E11-F TTGGTGGTGTCATCCTCTTT

Matches = 12

Score = 7

TTGGTNNTGTCNNCCTN

est. tm =  21.5 oC

DeltaG @37 degrees =  -5.69 kcal/mole

 

                    5'-TTGGTGGTGTCATCCTCTTT-3'

                       |||||xx||||xx|||x

                 3'-GTCAACCAAGACAGAGGGAC-5'

 

GATA24H01N-F CAGGGAGACAGAACCAACTG versus GATA151F03N-F CTGGACAGTCAGTTGGAGCT

Matches = 9

Score = 7

AGNNCCAACTG

est. tm =  8.3 oC

DeltaG @37 degrees =  -3.90 kcal/mole

 

                    3'-TCGAGGTTGACTGACAGGTC-5'

                       ||xx|||||||

           5'-CAGGGAGACAGAACCAACTG-3'

 

ATA2A03-F CCACGTGACTTTCATTAGGG versus GATA88H02N-F TGAGTTTTTCCTATTGAGAGTCC

Matches = 11

Score = 7

NGACTNTCANTAGGN

est. tm =  17.4 oC

DeltaG @37 degrees =  -5.12 kcal/mole

 

                    3'-CCTGAGAGTTATCCTTTTTGAGT-5'

                       x||||x|||x||||x

               5'-CCACGTGACTTTCATTAGGG-3'

 

ATA2A03-F CCACGTGACTTTCATTAGGG versus ATA21F01-R ATGACCAAAATGAAAATCACTG

Matches = 13

Score = 8

NNGTGANTTTCATTNNGG

est. tm =  19.7 oC

DeltaG @37 degrees =  -4.71 kcal/mole

 

                    3'-GTCACTAAAAGTAAAACCAGTA-5'

                       xx||||x|||||||xx||

                  5'-CCACGTGACTTTCATTAGGG-3'

 

ATA2A03-F CCACGTGACTTTCATTAGGG versus ATA79C10-F GCCTTCATGGAGGTTACATG

Matches = 13

Score = 7

CANGTNACNTNCATNANGG

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                    3'-GTACATTGGAGGTACTTCCG-5'

                       ||x||x||x|x|||x|x||

                   5'-CCACGTGACTTTCATTAGGG-3'

 

ATA2A03-F CCACGTGACTTTCATTAGGG versus GATA6B09-F TTGCATCCATACGGAAAGTC

Matches = 11

Score = 8

GACTTTCNNTANGG

est. tm =  7.1 oC

DeltaG @37 degrees =  -2.74 kcal/mole

 

                    3'-CTGAAAGGCATACCTACGTT-5'

                       |||||||xx||x||

              5'-CCACGTGACTTTCATTAGGG-3'

 

ATA2A03-F CCACGTGACTTTCATTAGGG versus GGAA20C10-R TTCCTGTCCTCTAATGTAACTTACC

Matches = 12

Score = 7

NGTNANTTNCATTAGNG

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                    3'-CCATTCAATGTAATCTCCTGTCCTT-5'

                       x||x|x||x||||||x|

                 5'-CCACGTGACTTTCATTAGGG-3'

 

ATA2A03-F CCACGTGACTTTCATTAGGG versus GATA188F04-R TACAGTAAATCACTTGGTAGGAGA

Matches = 12

Score = 7

TANNGNAANTCACNTGG

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.34 kcal/mole

 

                    5'-TACAGTAAATCACTTGGTAGGAGA-3'

                       ||xx|x||x||||x|||

                 3'-GGGATTACTTTCAGTGCACC-5'

 

GATA62A12-F AACTAGGCTGCTTCCCAGAT versus GATA41A01-F GTGTTGACCAACTGGGTAGC

Matches = 9

Score = 7

GCTNCCCAGNT

est. tm =  5.2 oC

DeltaG @37 degrees =  -4.03 kcal/mole

 

                    3'-CGATGGGTCAACCAGTTGTG-5'

                       |||x|||||x|

           5'-AACTAGGCTGCTTCCCAGAT-3'

 

GATA62A12-F AACTAGGCTGCTTCCCAGAT versus GGAA3C07-F ATGTACATGTGTCTGGGAAGG

Matches = 9

Score = 7

NCTTCCCAGAN

est. tm =  29.8 oC

DeltaG @37 degrees =  -7.84 kcal/mole

 

                    3'-GGAAGGGTCTGTGTACATGTA-5'

                       x|||||||||x

           5'-AACTAGGCTGCTTCCCAGAT-3'

 

GATA107-F GACTCCAAATCACATGAGCC versus GATA175D03-F TGGAGTCTCTGGGTGAAGAG

Matches = 7

Score = 7

TGGAGTC

est. tm =  8.8 oC

DeltaG @37 degrees =  -4.69 kcal/mole

 

                    5'-TGGAGTCTCTGGGTGAAGAG-3'

                       |||||||

       3'-CCGAGTACACTAAACCTCAG-5'

 

GATA107-F GACTCCAAATCACATGAGCC versus GATA163B10N-R TGGAGACAGCATGTGATTTG

Matches = 11

Score = 7

CAAATCACATGNNNN

est. tm =  24.4 oC

DeltaG @37 degrees =  -5.99 kcal/mole

 

                    3'-GTTTAGTGTACGACAGAGGT-5'

                       |||||||||||xxxx

               5'-GACTCCAAATCACATGAGCC-3'

 

GATA107-F GACTCCAAATCACATGAGCC versus GGAA8G07-R CCCTGGACTCATGGTACTTG

Matches = 11

Score = 7

CAANTNNCATGAGNC

est. tm =  less than zero

DeltaG @37 degrees =  -1.11 kcal/mole

 

                    3'-GTTCATGGTACTCAGGTCCC-5'

                       |||x|xx||||||x|

               5'-GACTCCAAATCACATGAGCC-3'

 

GATA27G03-F GAGCCAGACCTTGTCTCAAA versus ATA29G03Z-R ATTTGGAATAAGGCCTGACT

Matches = 14

Score = 9

AGNCAGNCCTTNTNNCAAA

est. tm =  6.6 oC

DeltaG @37 degrees =  -2.25 kcal/mole

 

                    3'-TCAGTCCGGAATAAGGTTTA-5'

                       ||x|||x||||x|xx||||

                   5'-GAGCCAGACCTTGTCTCAAA-3'

 

GATA12F04-F AGCCATCTGGGCTTAGAGAT versus GATA42H02-F AGCACAAGATTCTCTAAGCCC

Matches = 11

Score = 10

GGGCTTAGAGAN

est. tm =  38.1 oC

DeltaG @37 degrees =  -9.64 kcal/mole

 

                    3'-CCCGAATCTCTTAGAACACGA-5'

                       |||||||||||x

            5'-AGCCATCTGGGCTTAGAGAT-3'

 

GATA87E02N-F GTTGTTGCTGGGCTTAGAAA versus GATA42H02-F AGCACAAGATTCTCTAAGCCC

Matches = 10

Score = 9

GGGCTTAGANA

est. tm =  20.5 oC

DeltaG @37 degrees =  -5.89 kcal/mole

 

                    3'-CCCGAATCTCTTAGAACACGA-5'

                       |||||||||x|

           5'-GTTGTTGCTGGGCTTAGAAA-3'

 

GATA42H02-F AGCACAAGATTCTCTAAGCCC versus GGAT1A4-R ATAGTTTTGTGCTTATTGACATGA

Matches = 10

Score = 7

ANANTNTTGTGCT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.99 kcal/mole

 

                     5'-ATAGTTTTGTGCTTATTGACATGA-3'

                        |x|x|x|||||||

             3'-CCCGAATCTCTTAGAACACGA-5'

 

GATA4G12-R GCCATCTGTACTGTTCCCAG versus GATA5B02-F AATCCCTGGGTACATTATATTTG

Matches = 13

Score = 8

CANNTNTANTGTNCCCAG

est. tm =  5.8 oC

DeltaG @37 degrees =  -2.37 kcal/mole

 

                    3'-GTTTATATTACATGGGTCCCTAA-5'

                       ||xx|x||x|||x|||||

                  5'-GCCATCTGTACTGTTCCCAG-3'

 

GATA5B02-F AATCCCTGGGTACATTATATTTG versus GGAA20C10-R TTCCTGTCCTCTAATGTAACTTACC

Matches = 11

Score = 7

GGTANNTTANATTNG

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                       3'-CCATTCAATGTAATCTCCTGTCCTT-5'

                          ||||xx|||x|||x|

               5'-AATCCCTGGGTACATTATATTTG-3'

 

GATA145D10N-F CCTGGAAGAACCAATGCTTA versus GATA6A05-R TTGTAATTCTTGGTCCTGCC

Matches = 12

Score = 7

GGNAGNACCAANNNTTA

est. tm =  1.5 oC

DeltaG @37 degrees =  -1.70 kcal/mole

 

                    3'-CCGTCCTGGTTCTTAATGTT-5'

                       ||x||x|||||xxx|||

                 5'-CCTGGAAGAACCAATGCTTA-3'

 

GATA145D10N-F CCTGGAAGAACCAATGCTTA versus GATA6G12-R GAAGACCTACGGATTGGGTC

Matches = 10

Score = 7

GANCCAATNCNTA

est. tm =  less than zero

DeltaG @37 degrees =  -0.23 kcal/mole

 

                    3'-CTGGGTTAGGCATCCAGAAG-5'

                       ||x|||||x|x||

             5'-CCTGGAAGAACCAATGCTTA-3'

 

GATA145D10N-F CCTGGAAGAACCAATGCTTA versus GATA6E05-R GTTCTTCAGGGAAACAGAACC

Matches = 9

Score = 7

GTTCTTCNNGG

est. tm =  6.8 oC

DeltaG @37 degrees =  -3.39 kcal/mole

 

                    5'-GTTCTTCAGGGAAACAGAACC-3'

                       |||||||xx||

           3'-ATTCGTAACCAAGAAGGTCC-5'

 

GATA90D07N-R CCCGGCCTTGATTTATTTAA versus GATA3E10-F TGTAAACTTCATGAAGGCAGG

Matches = 13

Score = 7

CCNGCCTTNATNNANTTNA

est. tm =  less than zero

DeltaG @37 degrees =  -0.33 kcal/mole

 

                    3'-GGACGGAAGTACTTCAAATGT-5'

                       ||x|||||x||xx|x||x|

                   5'-CCCGGCCTTGATTTATTTAA-3'

 

GATA3E10-F TGTAAACTTCATGAAGGCAGG versus GATA50C03N-R CAAGTTTACAGTGAACTGTGGTCA

Matches = 9

Score = 8

NAAGTTTACA

est. tm =  16.6 oC

DeltaG @37 degrees =  -5.05 kcal/mole

 

                     5'-CAAGTTTACAGTGAACTGTGGTCA-3'

                        x|||||||||

          3'-GGACGGAAGTACTTCAAATGT-5'

 

ATA79C10-F GCCTTCATGGAGGTTACATG versus GATA3E10-F TGTAAACTTCATGAAGGCAGG

Matches = 16

Score = 14

TGTAANCTNCATGAAGGC

est. tm =  37.1 oC

DeltaG @37 degrees =  -9.42 kcal/mole

 

                    5'-TGTAAACTTCATGAAGGCAGG-3'

                       |||||x||x|||||||||

                  3'-GTACATTGGAGGTACTTCCG-5'

 

UT6540-F TTCCTCTGGCTTCTAGAAG versus GATA3E10-F TGTAAACTTCATGAAGGCAGG

Matches = 11

Score = 7

NCTGNCTTCNNGAAG

est. tm =  9.1 oC

DeltaG @37 degrees =  -3.19 kcal/mole

 

                   3'-GGACGGAAGTACTTCAAATGT-5'

                      x|||x||||xx||||

               5'-TTCCTCTGGCTTCTAGAAG-3'

 

GATA67D03-F TTAAAGGAGTGATCTCCCCC versus Mfd232-F AGAGTGAGATTCCACCTTTC

Matches = 13

Score = 7

NAAAGGNGNNATCTCNCNC

est. tm =  0.2 oC

DeltaG @37 degrees =  -1.22 kcal/mole

 

                    3'-CTTTCCACCTTAGAGTGAGA-5'

                       x|||||x|xx|||||x|x|

                   5'-TTAAAGGAGTGATCTCCCCC-3'

 

GATA52A12-F TTATAGAGCAGCCTTCCCTG versus GGAA3C07-F ATGTACATGTGTCTGGGAAGG

Matches = 8

Score = 7

CCTTCCCNG

est. tm =  6.6 oC

DeltaG @37 degrees =  -4.23 kcal/mole

 

                    3'-GGAAGGGTCTGTGTACATGTA-5'

                       |||||||x|

         5'-TTATAGAGCAGCCTTCCCTG-3'

 

GATA3H06-F CTTTCCCTCTCTTCCCACAT versus GATA43A04-F GGAAAGCCTGTAGGAAGAGG

Matches = 10

Score = 7

NCTCTTCCNACAN

est. tm =  23.7 oC

DeltaG @37 degrees =  -6.50 kcal/mole

 

                    3'-GGAGAAGGATGTCCGAAAGG-5'

                       x|||||||x|||x

             5'-CTTTCCCTCTCTTCCCACAT-3'

 

GATA3H06-F CTTTCCCTCTCTTCCCACAT versus GATA49D12N-R AGATAGAGGGAAAGGCATATGTG

Matches = 11

Score = 8

NNANAGAGGGAAAG

est. tm =  23.1 oC

DeltaG @37 degrees =  -6.07 kcal/mole

 

                    5'-AGATAGAGGGAAAGGCATATGTG-3'

                       xx|x||||||||||

              3'-TACACCCTTCTCTCCCTTTC-5'

 

GATA62A04-F TAAGTGCCAGAGTCTCCCAC versus GATA62A04-F TAAGTGCCAGAGTCTCCCAC

Matches = 12

Score = 7

GTGNNAGANTCTNNCAC

est. tm =  6.0 oC

DeltaG @37 degrees =  -2.31 kcal/mole

 

                    3'-CACCCTCTGAGACCGTGAAT-5'

                       |||xx|||x|||xx|||

                 5'-TAAGTGCCAGAGTCTCCCAC-3'

 

GATA62A04-F TAAGTGCCAGAGTCTCCCAC versus AGAT125-R TGGCAAAGTCAGGCCATTAT

Matches = 13

Score = 7

TGGNANANTCNGGCNNTTA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.47 kcal/mole

 

                    5'-TGGCAAAGTCAGGCCATTAT-3'

                       |||x|x|x||x|||xx|||

                   3'-CACCCTCTGAGACCGTGAAT-5'

 

ATA23A10-F TTGGGAAGAATAGCTTTCCC versus GATA62F03N-F TTCCCAAAAGTTGCCATCTA

Matches = 7

Score = 7

TTCCCAA

est. tm =  5.7 oC

DeltaG @37 degrees =  -4.43 kcal/mole

 

                    5'-TTCCCAAAAGTTGCCATCTA-3'

                       |||||||

       3'-CCCTTTCGATAAGAAGGGTT-5'

 

GATA11A11-F TATCGAATTGTAACCCCGTT versus GATA168F06-F ACAATTCCAGAAACTTCCCC

Matches = 9

Score = 7

ACAATTCNANA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.18 kcal/mole

 

                    5'-ACAATTCCAGAAACTTCCCC-3'

                       |||||||x|x|

           3'-TTGCCCCAATGTTAAGCTAT-5'

 

AAAT072-F GGCAGCAAGAGTGAAACTCA versus GATA8B01-R ATGGATAAATTGAGTTTCCCC

Matches = 9

Score = 7

NGNGAAACTCA

est. tm =  8.5 oC

DeltaG @37 degrees =  -3.93 kcal/mole

 

                    3'-CCCCTTTGAGTTAAATAGGTA-5'

                       x|x||||||||

           5'-GGCAGCAAGAGTGAAACTCA-3'

 

AAAT072-F GGCAGCAAGAGTGAAACTCA versus ATA4E02-R CTGCTTTGGGTTTCACTTGT

Matches = 11

Score = 8

ANNAGTGAAACNCA

est. tm =  8.3 oC

DeltaG @37 degrees =  -3.26 kcal/mole

 

                    3'-TGTTCACTTTGGGTTTCGTC-5'

                       |xx||||||||x||

              5'-GGCAGCAAGAGTGAAACTCA-3'

 

AAAT072-F GGCAGCAAGAGTGAAACTCA versus GATA22F11NZ-R TCTCTCTTGCTCCACTGCAA

Matches = 11

Score = 8

TCNCTCTTGCTNCN

est. tm =  18.7 oC

DeltaG @37 degrees =  -5.43 kcal/mole

 

                    5'-TCTCTCTTGCTCCACTGCAA-3'

                       ||x||||||||x|x

              3'-ACTCAAAGTGAGAACGACGG-5'

 

AAAT072-F GGCAGCAAGAGTGAAACTCA versus GATA165B12-R TTAAAATCATTTTCACTGTGTATGC

Matches = 13

Score = 7

GCANNNANAGTGAAANTNA

est. tm =  2.1 oC

DeltaG @37 degrees =  -1.49 kcal/mole

 

                    3'-CGTATGTGTCACTTTTACTAAAATT-5'

                       |||xxx|x|||||||x|x|

                   5'-GGCAGCAAGAGTGAAACTCA-3'

 

GATA6G12-R GAAGACCTACGGATTGGGTC versus TTA032Z-F GGTCTTCCTTGTGGTTTTTCA

Matches = 7

Score = 7

GGTCTTC

est. tm =  7.6 oC

DeltaG @37 degrees =  -4.31 kcal/mole

 

                    5'-GGTCTTCCTTGTGGTTTTTCA-3'

                       |||||||

       3'-CTGGGTTAGGCATCCAGAAG-5'

 

GATA42H02-R AAAGCCAAAAGATGATGCAA versus ATA50C05-F TTTGGAAAGAATCTGTATTATCTTT

Matches = 10

Score = 7

AAAGATNATNCAN

est. tm =  less than zero

DeltaG @37 degrees =  -2.35 kcal/mole

 

                    3'-TTTCTATTATGTCTAAGAAAGGTTT-5'

                       ||||||x||x||x

             5'-AAAGCCAAAAGATGATGCAA-3'

 

ATA50C05-F TTTGGAAAGAATCTGTATTATCTTT versus GGAA9D03-R TTCTTTCCTTACTAAGTTGAGAACG

Matches = 9

Score = 7

TTCTTTCCNNA

est. tm =  1.5 oC

DeltaG @37 degrees =  -2.84 kcal/mole

 

                         5'-TTCTTTCCTTACTAAGTTGAGAACG-3'

                            ||||||||xx|

           3'-TTTCTATTATGTCTAAGAAAGGTTT-5'

 

ATA50C05-F TTTGGAAAGAATCTGTATTATCTTT versus GATA131D09-F AAAAATCTGACTCTATTCAAATGTT

Matches = 14

Score = 7

ANAANNCNGANTCTNTNCAAA

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                         5'-AAAAATCTGACTCTATTCAAATGTT-3'

                            |x||xx|x||x|||x|x||||

                     3'-TTTCTATTATGTCTAAGAAAGGTTT-5'

 

GATA21D04-R TGCTTGGCACACTTCAGG versus GATA163B10N-F CATTTCAAACCCCTGAAGTG

Matches = 9

Score = 9

CACTTCAGG

est. tm =  24.5 oC

DeltaG @37 degrees =  -6.63 kcal/mole

 

                  3'-GTGAAGTCCCCAAACTTTAC-5'

                     |||||||||

         5'-TGCTTGGCACACTTCAGG-3'

 

GATA163B10N-F CATTTCAAACCCCTGAAGTG versus GATA68A03-R TTGAAATGGGCACAGAAATT

Matches = 8

Score = 8

TTGAAATG

est. tm =  8.4 oC

DeltaG @37 degrees =  -3.90 kcal/mole

 

                    5'-TTGAAATGGGCACAGAAATT-3'

                       ||||||||

        3'-GTGAAGTCCCCAAACTTTAC-5'

 

GATA163B10N-F CATTTCAAACCCCTGAAGTG versus ATA33B03Z-R TAGTATCCACTTCCTGGGGT

Matches = 10

Score = 7

ANCCCNNGAAGTG

est. tm =  5.4 oC

DeltaG @37 degrees =  -3.33 kcal/mole

 

                    3'-TGGGGTCCTTCACCTATGAT-5'

                       |x|||xx||||||

             5'-CATTTCAAACCCCTGAAGTG-3'

 

GATA6C01-R TGGCACTAGATGTTTTCAAGG versus GATA11E02N-F TTGCCTTGTTAACATGTACAGG

Matches = 13

Score = 7

NCNNTANATGTTNNCAAGG

est. tm =  8.0 oC

DeltaG @37 degrees =  -2.27 kcal/mole

 

                     3'-GGACATGTACAATTGTTCCGTT-5'

                        x|xx||x|||||xx|||||

                   5'-TGGCACTAGATGTTTTCAAGG-3'

 

ATA28B11-F CCTGATGGTCATGTGTCTGA versus GATA63A03N-F GTGGATAGACACATGACAGATAGG

Matches = 14

Score = 8

CCTNNNNGTCATGTGTCTNN

est. tm =  32.8 oC

DeltaG @37 degrees =  -8.05 kcal/mole

 

                    3'-GGATAGACAGTACACAGATAGGTG-5'

                       |||xxxx|||||||||||xx

                    5'-CCTGATGGTCATGTGTCTGA-3'

 

ATA11D10-F TTGGAAGACATTTGCAGTCA versus GATA87E02N-R TTGATTGACTGCACTTTTCA

Matches = 11

Score = 7

NGANANNTGCAGTCA

est. tm =  17.7 oC

DeltaG @37 degrees =  -5.13 kcal/mole

 

                    3'-ACTTTTCACGTCAGTTAGTT-5'

                       x||x|xx||||||||

               5'-TTGGAAGACATTTGCAGTCA-3'

 

GATA23F08-F GAATGCAGGACTGTTTCTGG versus GATA81D12-R TAATGTACCTGGTACCAAAAACA

Matches = 8

Score = 7

TGTTTNTGG

est. tm =  1.1 oC

DeltaG @37 degrees =  -3.30 kcal/mole

 

                    3'-ACAAAAACCATGGTCCATGTAAT-5'

                       |||||x|||

         5'-GAATGCAGGACTGTTTCTGG-3'

 

GATA23F08-F GAATGCAGGACTGTTTCTGG versus SDF1-F GGCCTGAATTCATTTATTATTATTCTAATA

Matches = 9

Score = 7

GNCCTGNATTC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.94 kcal/mole

 

                    5'-GGCCTGAATTCATTTATTATTATTCTAATA-3'

                       |x||||x||||

           3'-GGTCTTTGTCAGGACGTAAG-5'

 

ATA6C09-F ACTTGAATCCAGGCATTCAG versus ATCT003-R AGGATGCCACTAAATGCCTA

Matches = 9

Score = 7

NAGGCATTNAG

est. tm =  15.2 oC

DeltaG @37 degrees =  -5.12 kcal/mole

 

                    3'-ATCCGTAAATCACCGTAGGA-5'

                       x|||||||x||

           5'-ACTTGAATCCAGGCATTCAG-3'

 

ATA6C09-F ACTTGAATCCAGGCATTCAG versus GATA43C11-F TTCAAGTGGTTGGGTTCACT

Matches = 7

Score = 7

TTCAAGT

est. tm =  0.7 oC

DeltaG @37 degrees =  -3.52 kcal/mole

 

                    5'-TTCAAGTGGTTGGGTTCACT-3'

                       |||||||

       3'-GACTTACGGACCTAAGTTCA-5'

 

ATA6C09-F ACTTGAATCCAGGCATTCAG versus SDF1-F GGCCTGAATTCATTTATTATTATTCTAATA

Matches = 11

Score = 7

NGCCTGNATTCANNT

est. tm =  14.9 oC

DeltaG @37 degrees =  -4.62 kcal/mole

 

                    5'-GGCCTGAATTCATTTATTATTATTCTAATA-3'

                       x|||||x|||||xx|

               3'-GACTTACGGACCTAAGTTCA-5'

 

ATA6C09-F ACTTGAATCCAGGCATTCAG versus GATA172D05-R ATAATTGAAAGCCCGGATTC

Matches = 13

Score = 10

GAATCCNGGCNTTCAN

est. tm =  31.6 oC

DeltaG @37 degrees =  -7.92 kcal/mole

 

                    3'-CTTAGGCCCGAAAGTTAATA-5'

                       ||||||x|||x||||x

                5'-ACTTGAATCCAGGCATTCAG-3'

 

ATA22G07-F CGTTCTGCACATGTATCCTG versus ATA79C10-F GCCTTCATGGAGGTTACATG

Matches = 9

Score = 7

CATGTANCCTN

est. tm =  11.8 oC

DeltaG @37 degrees =  -4.49 kcal/mole

 

                    3'-GTACATTGGAGGTACTTCCG-5'

                       ||||||x|||x

           5'-CGTTCTGCACATGTATCCTG-3'

 

ATA22G07-F CGTTCTGCACATGTATCCTG versus GGAA2F11N-F AAGATACACAGGAAGAAAGAAAGG

Matches = 13

Score = 7

ANGATACANNNGNAGAANG

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                    5'-AAGATACACAGGAAGAAAGAAAGG-3'

                       |x||||||xxx|x||||x|

                   3'-GTCCTATGTACACGTCTTGC-5'

 

UT254-F TCTCAGTGAAGGTAGACAGGA versus AFMb035xb9-F AACCTCAAGTCTACATTCATAGCGG

Matches = 14

Score = 7

NCNCNNTGAANGTAGACNNGA

est. tm =  4.6 oC

DeltaG @37 degrees =  -1.47 kcal/mole

 

                     3'-GGCGATACTTACATCTGAACTCCAA-5'

                        x|x|xx||||x||||||xx||

                     5'-TCTCAGTGAAGGTAGACAGGA-3'

 

GATA26G09N-R ACATGAGCTGGTTCCCCTATGA versus AFMb035xb9-F AACCTCAAGTCTACATTCATAGCGG

Matches = 8

Score = 7

CCNCTATGA

est. tm =  less than zero

DeltaG @37 degrees =  -2.43 kcal/mole

 

                      3'-GGCGATACTTACATCTGAACTCCAA-5'

                         ||x||||||

         5'-ACATGAGCTGGTTCCCCTATGA-3'

 

GGAA4A12-R CGAATGCATACTTGCTGTTG versus AFMb035xb9-F AACCTCAAGTCTACATTCATAGCGG

Matches = 14

Score = 9

AACNNCAAGTNTNCATTCN

est. tm =  18.0 oC

DeltaG @37 degrees =  -4.37 kcal/mole

 

                    5'-AACCTCAAGTCTACATTCATAGCGG-3'

                       |||xx|||||x|x|||||x

                   3'-GTTGTCGTTCATACGTAAGC-5'

 

AAT034-F CCCATCTATGAATGCACACG versus AFMb035xb9-F AACCTCAAGTCTACATTCATAGCGG

Matches = 13

Score = 8

CNNCTATGAATGNANACN

est. tm =  9.7 oC

DeltaG @37 degrees =  -2.69 kcal/mole

 

                    3'-GGCGATACTTACATCTGAACTCCAA-5'

                       |xx|||||||||x|x||x

                  5'-CCCATCTATGAATGCACACG-3'

 

GATA3C02-R CTGGCCCCATCTCTCTCTAT versus GATA119B03-F ACCCCTGAGATGATAGATAGACA

Matches = 9

Score = 7

TNTCTNTCTAT

est. tm =  less than zero

DeltaG @37 degrees =  -0.72 kcal/mole

 

                    3'-ACAGATAGATAGTAGAGTCCCCA-5'

                       |x|||x|||||

           5'-CTGGCCCCATCTCTCTCTAT-3'

 

GATA119B03-F ACCCCTGAGATGATAGATAGACA versus GATA188F04-F AACAGAACCAATAGGCTATCTATC

Matches = 10

Score = 8

GATAGATAGNCN

est. tm =  9.0 oC

DeltaG @37 degrees =  -3.39 kcal/mole

 

                       3'-CTATCTATCGGATAACCAAGACAA-5'

                          |||||||||x|x

            5'-ACCCCTGAGATGATAGATAGACA-3'

 

GATA119B03-F ACCCCTGAGATGATAGATAGACA versus GATA22G05-R AAAACAAATATGGCTCTATCTATCG

Matches = 10

Score = 7

NGATAGATAGANN

est. tm =  18.3 oC

DeltaG @37 degrees =  -4.99 kcal/mole

 

                       3'-GCTATCTATCTCGGTATAAACAAAA-5'

                          x||||||||||xx

             5'-ACCCCTGAGATGATAGATAGACA-3'

 

GATA21F03-R CCTGCCTGCCTATCTATCTG versus GATA119B03-F ACCCCTGAGATGATAGATAGACA

Matches = 11

Score = 8

TGNCTATCTATCNN

est. tm =  13.1 oC

DeltaG @37 degrees =  -3.83 kcal/mole

 

                    3'-ACAGATAGATAGTAGAGTCCCCA-5'

                       ||x|||||||||xx

              5'-CCTGCCTGCCTATCTATCTG-3'

 

GATA41G07-F AAGCAATGCAGTGATCCATT versus GGAA23C05N-F GGAATAAGTGCAGTGCTTGG

Matches = 13

Score = 8

NGNATNANTGCANTGCTT

est. tm =  12.6 oC

DeltaG @37 degrees =  -3.93 kcal/mole

 

                    5'-GGAATAAGTGCAGTGCTTGG-3'

                       x|x||x|x||||x|||||

                  3'-TTACCTAGTGACGTAACGAA-5'

 

GATA41G07-F AAGCAATGCAGTGATCCATT versus GATA50C03N-F GGATGATCACAATGCTTATGG

Matches = 12

Score = 7

GGATNANNNCANTGCTT

est. tm =  6.6 oC

DeltaG @37 degrees =  -2.69 kcal/mole

 

                    5'-GGATGATCACAATGCTTATGG-3'

                       ||||x|xxx||x|||||

                 3'-TTACCTAGTGACGTAACGAA-5'

 

GATA41G07-F AAGCAATGCAGTGATCCATT versus ATA23G05-R TGCATTGCATGAAAGTAGGA

Matches = 9

Score = 8

TGCATTGCNT

est. tm =  16.5 oC

DeltaG @37 degrees =  -5.46 kcal/mole

 

                    5'-TGCATTGCATGAAAGTAGGA-3'

                       ||||||||x|

          3'-TTACCTAGTGACGTAACGAA-5'

 

GATA41G07-F AAGCAATGCAGTGATCCATT versus GATA68F07-F ATTGCTTCCCCTTTACCTGA

Matches = 7

Score = 7

ATTGCTT

est. tm =  2.8 oC

DeltaG @37 degrees =  -3.85 kcal/mole

 

                    5'-ATTGCTTCCCCTTTACCTGA-3'

                       |||||||

       3'-TTACCTAGTGACGTAACGAA-5'

 

GATA13G11-F TTGGGACCTCTTATTTTCCA versus GATA73D10N-F TCATGACAAAACAGAAAATAAGTG

Matches = 10

Score = 7

CNCTTATTTTCNN

est. tm =  6.5 oC

DeltaG @37 degrees =  -2.70 kcal/mole

 

                    3'-GTGAATAAAAGACAAAACAGTACT-5'

                       |x|||||||||xx

             5'-TTGGGACCTCTTATTTTCCA-3'

 

GATA13G11-F TTGGGACCTCTTATTTTCCA versus GATA169E06-F ACAGTTCCCAAGACTAAACAGC

Matches = 9

Score = 7

ANAGNTCCCAA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.78 kcal/mole

 

                    5'-ACAGTTCCCAAGACTAAACAGC-3'

                       |x||x||||||

           3'-ACCTTTTATTCTCCAGGGTT-5'

 

GATA70B08-R GTCCCAAAGGACCTGCTC versus GATA13G11-F TTGGGACCTCTTATTTTCCA

Matches = 7

Score = 7

TTGGGAC

est. tm =  10.0 oC

DeltaG @37 degrees =  -4.93 kcal/mole

 

                  5'-TTGGGACCTCTTATTTTCCA-3'

                     |||||||

       3'-CTCGTCCAGGAAACCCTG-5'

 

GATA24D12-F CCCTAACTCCCATGTTGATG versus UT5029-R AAATCATCAGTGGAGTTAGCA

Matches = 14

Score = 8

NNCTAACTCCNNTGNTGATN

est. tm =  26.1 oC

DeltaG @37 degrees =  -6.26 kcal/mole

 

                    3'-ACGATTGAGGTGACTACTAAA-5'

                       xx||||||||xx||x||||x

                    5'-CCCTAACTCCCATGTTGATG-3'

 

GATA24D12-F CCCTAACTCCCATGTTGATG versus GGAA20C10-F AGCAAACTTCATCCACTTGG

Matches = 9

Score = 7

CCANGTNGATG

est. tm =  less than zero

DeltaG @37 degrees =  -2.56 kcal/mole

 

                    3'-GGTTCACCTACTTCAAACGA-5'

                       |||x||x||||

           5'-CCCTAACTCCCATGTTGATG-3'

 

GATA73D10N-F TCATGACAAAACAGAAAATAAGTG versus AAT243-R TCACCCATTAATTCTTTGATTTGA

Matches = 13

Score = 7

NCAAANCANANAATNANTG

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                        3'-AGTTTAGTTTCTTAATTACCCACT-5'

                           x||||x||x|x|||x|x||

                   5'-TCATGACAAAACAGAAAATAAGTG-3'

 

GATA5D08-F ACAAAACCCCAAGTACGTGA versus GATA32F05-F ATTTCACGTACTTGGCAAGC

Matches = 12

Score = 7

NNNNNCCAAGTACGTGA

est. tm =  31.1 oC

DeltaG @37 degrees =  -7.67 kcal/mole

 

                    3'-CGAACGGTTCATGCACTTTA-5'

                       xxxxx||||||||||||

                 5'-ACAAAACCCCAAGTACGTGA-3'

 

GATA5D08-F ACAAAACCCCAAGTACGTGA versus GGAA22G01-R TCGTTGAGATTTTGTGCTATG

Matches = 11

Score = 7

TNNTTGNGNTTTTGT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.55 kcal/mole

 

                    5'-TCGTTGAGATTTTGTGCTATG-3'

                       |xx|||x|x||||||

               3'-AGTGCATGAACCCCAAAACA-5'

 

GATA41G07-R AAAATCCTTTCAACAAATAGAAGC versus GGAA6D03N-F ATAGGATTTTATCACTCACAGATTT

Matches = 9

Score = 8

ANAGGATTTT

est. tm =  2.3 oC

DeltaG @37 degrees =  -3.08 kcal/mole

 

                        5'-ATAGGATTTTATCACTCACAGATTT-3'

                           |x||||||||

          3'-CGAAGATAAACAACTTTCCTAAAA-5'

 

TTCA004-R CCCAGAGCCTAAATCTGTGC versus GGAA6D03N-F ATAGGATTTTATCACTCACAGATTT

Matches = 9

Score = 8

AAATCTGTGN

est. tm =  21.2 oC

DeltaG @37 degrees =  -6.03 kcal/mole

 

                    3'-TTTAGACACTCACTATTTTAGGATA-5'

                       |||||||||x

          5'-CCCAGAGCCTAAATCTGTGC-3'

 

ATA27D04N-R AGTGGGTAGATCCTATGTGAGC versus GGAA6D03N-F ATAGGATTTTATCACTCACAGATTT

Matches = 12

Score = 8

ATAGGATNTNNNCACT

est. tm =  8.2 oC

DeltaG @37 degrees =  -2.81 kcal/mole

 

                      5'-ATAGGATTTTATCACTCACAGATTT-3'

                         |||||||x|xxx||||

                3'-CGAGTGTATCCTAGATGGGTGA-5'

 

GATA8A05-R TTTAACAGACAAATGACAAATCTG versus GGAA6D03N-F ATAGGATTTTATCACTCACAGATTT

Matches = 7

Score = 7

AAATCTG

est. tm =  less than zero

DeltaG @37 degrees =  -3.00 kcal/mole

 

                        3'-TTTAGACACTCACTATTTTAGGATA-5'

                           |||||||

       5'-TTTAACAGACAAATGACAAATCTG-3'

 

GATA63F08-F TCTCTTACCCTTTGGGACCT versus GATA3H06-R CCTGGGGGTAAGAGGAGATA

Matches = 11

Score = 7

CCNNNGGGTAAGAGN

est. tm =  22.9 oC

DeltaG @37 degrees =  -6.04 kcal/mole

 

                    5'-CCTGGGGGTAAGAGGAGATA-3'

                       ||xxx|||||||||x

               3'-TCCAGGGTTTCCCATTCTCT-5'

 

ATA78D02N-R GGCAAGAGAACTCACGTAA versus GATA63F08-F TCTCTTACCCTTTGGGACCT

Matches = 8

Score = 7

TCTCTTNCC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -3.12 kcal/mole

 

                   5'-TCTCTTACCCTTTGGGACCT-3'

                      ||||||x||

         3'-AATGCACTCAAGAGAACGG-5'

 

GATA63F08-F TCTCTTACCCTTTGGGACCT versus GGAA20G04-R TTCACAAGGTTCCACAAGGT

Matches = 11

Score = 8

ACCNTNTGGNACCT

est. tm =  7.3 oC

DeltaG @37 degrees =  -3.94 kcal/mole

 

                    3'-TGGAACACCTTGGAACACTT-5'

                       |||x|x|||x||||

              5'-TCTCTTACCCTTTGGGACCT-3'

 

GATA189C06-F CCCCCATGAGTTATTCCTCT versus GATA8B01-F TGCCATAGCAGAGAGGGATA

Matches = 8

Score = 7

TATNCCTCT

est. tm =  less than zero

DeltaG @37 degrees =  -2.19 kcal/mole

 

                    3'-ATAGGGAGAGACGATACCGT-5'

                       |||x|||||

         5'-CCCCCATGAGTTATTCCTCT-3'

 

MFD442-GTTT002-F GGAGTGAGTCCACCTTCCAA versus GGAA17G05-R ACACCTGGATCTTGGACTCA

Matches = 12

Score = 8

TGAGTCCANNNTCCAN

est. tm =  31.7 oC

DeltaG @37 degrees =  -7.99 kcal/mole

 

                    3'-ACTCAGGTTCTAGGTCCACA-5'

                       ||||||||xxx||||x

                5'-GGAGTGAGTCCACCTTCCAA-3'

 

MFD442-GTTT002-F GGAGTGAGTCCACCTTCCAA versus ATA29G03Z-R ATTTGGAATAAGGCCTGACT

Matches = 13

Score = 8

AGTNAGNCCNNNTTCCAA

est. tm =  11.1 oC

DeltaG @37 degrees =  -3.36 kcal/mole

 

                    3'-TCAGTCCGGAATAAGGTTTA-5'

                       |||x||x||xxx||||||

                  5'-GGAGTGAGTCCACCTTCCAA-3'

 

TTCA004-F CACCCAGCCCCTATGTTTTA versus ATAA018Z-F CTGGGTGACAGAGCAAGACC

Matches = 7

Score = 7

CTGGGTG

est. tm =  13.8 oC

DeltaG @37 degrees =  -5.41 kcal/mole

 

                    5'-CTGGGTGACAGAGCAAGACC-3'

                       |||||||

       3'-ATTTTGTATCCCCGACCCAC-5'

 

ATAA018Z-F CTGGGTGACAGAGCAAGACC versus ATA3A07-F GTCTGTAACCTAGGCAGGCA

Matches = 10

Score = 7

NTCTGTNACCNAG

est. tm =  11.0 oC

DeltaG @37 degrees =  -4.26 kcal/mole

 

                    5'-GTCTGTAACCTAGGCAGGCA-3'

                       x|||||x|||x||

             3'-CCAGAACGAGACAGTGGGTC-5'

 

ATAA018Z-F CTGGGTGACAGAGCAAGACC versus GATA2A12-F TGTGTTTGGGTTTCCTCTGT

Matches = 10

Score = 7

ACAGAGNAANNCC

est. tm =  2.7 oC

DeltaG @37 degrees =  -2.79 kcal/mole

 

                    3'-TGTCTCCTTTGGGTTTGTGT-5'

                       ||||||x||xx||

             5'-CTGGGTGACAGAGCAAGACC-3'

 

ATAA018Z-F CTGGGTGACAGAGCAAGACC versus sRA-F CACTAGCACCCAGAACCGTC

Matches = 10

Score = 7

CNCTNNCACCCAG

est. tm =  8.8 oC

DeltaG @37 degrees =  -3.81 kcal/mole

 

                    5'-CACTAGCACCCAGAACCGTC-3'

                       |x||xx|||||||

             3'-CCAGAACGAGACAGTGGGTC-5'

 

ATAA018Z-F CTGGGTGACAGAGCAAGACC versus GATA27A03-F CAGTTACCCAGGAAGCTGAA

Matches = 9

Score = 7

CNGTNACCCAG

est. tm =  3.2 oC

DeltaG @37 degrees =  -3.48 kcal/mole

 

                    5'-CAGTTACCCAGGAAGCTGAA-3'

                       |x||x||||||

           3'-CCAGAACGAGACAGTGGGTC-5'

 

ATAA018Z-F CTGGGTGACAGAGCAAGACC versus GATA30G01-R AATGAGGTCTTACTTTGTTGCC

Matches = 13

Score = 9

GGNNACANAGNAAGACC

est. tm =  17.5 oC

DeltaG @37 degrees =  -4.60 kcal/mole

 

                    3'-CCGTTGTTTCATTCTGGAGTAA-5'

                       ||xx|||x||x||||||

                 5'-CTGGGTGACAGAGCAAGACC-3'

 

UT7129-F TAGAAGCCAGAAGAAGAGCA versus GATA89A11-F GCTTCTGAAAGCTTCTAGTTTACC

Matches = 12

Score = 7

NCTTCTNNNNGCTTCTA

est. tm =  19.5 oC

DeltaG @37 degrees =  -4.82 kcal/mole

 

                    5'-GCTTCTGAAAGCTTCTAGTTTACC-3'

                       x|||||xxxx|||||||

                 3'-ACGAGAAGAAGACCGAAGAT-5'

 

UT7129-F TAGAAGCCAGAAGAAGAGCA versus GATA81D05-F GTGGTCTTCTACTTGTGTCAATAC

Matches = 14

Score = 8

TGNTCTTCTNCTNGNNTCNA

est. tm =  less than zero

DeltaG @37 degrees =  -0.19 kcal/mole

 

                    5'-GTGGTCTTCTACTTGTGTCAATAC-3'

                        ||x||||||x||x|xx||x|

                     3'-ACGAGAAGAAGACCGAAGAT-5'

 

UT6540-F TTCCTCTGGCTTCTAGAAG versus UT7129-F TAGAAGCCAGAAGAAGAGCA

Matches = 14

Score = 13

TAGAAGCCAGANGAA

est. tm =  36.7 oC

DeltaG @37 degrees =  -9.27 kcal/mole

 

                   5'-TAGAAGCCAGAAGAAGAGCA-3'

                      |||||||||||x|||

               3'-GAAGATCTTCGGTCTCCTT-5'

 

UT7129-F TAGAAGCCAGAAGAAGAGCA versus GATA32B03-F AATAGTGACATTCTCTTCTTATGGC

Matches = 13

Score = 11

GCCANAAGAAGAGNA

est. tm =  28.0 oC

DeltaG @37 degrees =  -7.02 kcal/mole

 

                    3'-CGGTATTCTTCTCTTACAGTGATAA-5'

                       ||||x||||||||x|

               5'-TAGAAGCCAGAAGAAGAGCA-3'

 

UT7129-F TAGAAGCCAGAAGAAGAGCA versus GATA11C06N-F GATCCCAAGCTCTTCCTCTT

Matches = 10

Score = 7

NAGANGAAGAGCN

est. tm =  24.3 oC

DeltaG @37 degrees =  -6.59 kcal/mole

 

                    3'-TTCTCCTTCTCGAACCCTAG-5'

                       x|||x|||||||x

             5'-TAGAAGCCAGAAGAAGAGCA-3'

 

GATA8G10-F GGGACAGAGAAGCAGAGAAG versus GATA21D04-F TAGGTTCTGGGCATGTCTGT

Matches = 12

Score = 7

ACAGANANGCNNAGAAN

est. tm =  12.3 oC

DeltaG @37 degrees =  -3.95 kcal/mole

 

                    3'-TGTCTGTACGGGTCTTGGAT-5'

                       |||||x|x||xx||||x

                 5'-GGGACAGAGAAGCAGAGAAG-3'

 

GATA41A01-F GTGTTGACCAACTGGGTAGC versus GATA23D03N-R ATTCTTTGTCTCCCCAGTTG

Matches = 10

Score = 8

CAACTGGGNAGN

est. tm =  25.4 oC

DeltaG @37 degrees =  -6.87 kcal/mole

 

                    3'-GTTGACCCCTCTGTTTCTTA-5'

                       ||||||||x||x

            5'-GTGTTGACCAACTGGGTAGC-3'

 

GATA41A01-F GTGTTGACCAACTGGGTAGC versus GATA45B10N-F GTGGTGAACACCAATAAATGG

Matches = 9

Score = 7

NTGGTNAACAC

est. tm =  16.2 oC

DeltaG @37 degrees =  -5.26 kcal/mole

 

                    5'-GTGGTGAACACCAATAAATGG-3'

                       x||||x|||||

           3'-CGATGGGTCAACCAGTTGTG-5'

 

GATA41A01-F GTGTTGACCAACTGGGTAGC versus GATA12H10-R TTGGTCAACATGTCAATTTAGG

Matches = 10

Score = 9

TTGGTCAACAN

est. tm =  32.6 oC

DeltaG @37 degrees =  -8.33 kcal/mole

 

                    5'-TTGGTCAACATGTCAATTTAGG-3'

                       ||||||||||x

           3'-CGATGGGTCAACCAGTTGTG-5'

 

GATA41A01-F GTGTTGACCAACTGGGTAGC versus GATA63D12-R AATATTGACTTTCTCTTGCTACCC

Matches = 7

Score = 7

GGGTAGC

est. tm =  13.3 oC

DeltaG @37 degrees =  -5.32 kcal/mole

 

                    3'-CCCATCGTTCTCTTTCAGTTATAA-5'

                       |||||||

       5'-GTGTTGACCAACTGGGTAGC-3'

 

UT254-F TCTCAGTGAAGGTAGACAGGA versus GATA7G07-F TTTTTGTATTTCATGTGTACATTCG

Matches = 11

Score = 7

NGAANGTANACANGA

est. tm =  less than zero

DeltaG @37 degrees =  -1.75 kcal/mole

 

                     3'-GCTTACATGTGTACTTTATGTTTTT-5'

                        x|||x|||x|||x||

               5'-TCTCAGTGAAGGTAGACAGGA-3'

 

GATA7G07-F TTTTTGTATTTCATGTGTACATTCG versus GATA52B03-R AAGTAAACATGTATAGGAAAAAGCT

Matches = 15

Score = 8

ANGTANACATGNANNNNAAAAA

est. tm =  2.5 oC

DeltaG @37 degrees =  -0.30 kcal/mole

 

                         5'-AAGTAAACATGTATAGGAAAAAGCT-3'

                            |x|||x|||||x|xxxx|||||

                      3'-GCTTACATGTGTACTTTATGTTTTT-5'

 

GATA178G09-F ATGGGTGGATGTACAAATGG versus GATA7G07-F TTTTTGTATTTCATGTGTACATTCG

Matches = 11

Score = 7

NGNATGTACANATGN

est. tm =  13.4 oC

DeltaG @37 degrees =  -4.20 kcal/mole

 

                    3'-GCTTACATGTGTACTTTATGTTTTT-5'

                       x|x|||||||x|||x

               5'-ATGGGTGGATGTACAAATGG-3'

 

GGAA2F11N-F AAGATACACAGGAAGAAAGAAAGG versus GATA7G07-F TTTTTGTATTTCATGTGTACATTCG

Matches = 15

Score = 7

NTTTNNTNNTTCNTGTGTANNTT

est. tm =  6.3 oC

DeltaG @37 degrees =  -1.09 kcal/mole

 

                        5'-TTTTTGTATTTCATGTGTACATTCG-3'

                           x|||xx|xx|||x||||||xx||

                       3'-GGAAAGAAAGAAGGACACATAGAA-5'

 

GATA27A12-R TGGCACATGTACACCAGAAC versus GATA7G07-F TTTTTGTATTTCATGTGTACATTCG

Matches = 12

Score = 7

CNNATGTACACNNGAAN

est. tm =  14.2 oC

DeltaG @37 degrees =  -3.72 kcal/mole

 

                    3'-GCTTACATGTGTACTTTATGTTTTT-5'

                       |xx||||||||xx|||x

                 5'-TGGCACATGTACACCAGAAC-3'

 

GGAA4B09N-R CACACTCAAAATACATGAAGGC versus GATA7G07-F TTTTTGTATTTCATGTGTACATTCG

Matches = 13

Score = 7

TTNNTGTATTTNNNGTGTN

est. tm =  14.5 oC

DeltaG @37 degrees =  -3.41 kcal/mole

 

                      5'-TTTTTGTATTTCATGTGTACATTCG-3'

                         ||xx|||||||xxx||||x

                   3'-CGGAAGTACATAAAACTCACAC-5'

 

GATA50D10-F ATGCCTACAAAAACCAGTGC versus AGAT113Z-R CGCTACCACGGGTGTTTTTA

Matches = 11

Score = 7

TANAAANACCNGTGN

est. tm =  0.7 oC

DeltaG @37 degrees =  -2.37 kcal/mole

 

                    3'-ATTTTTGTGGGCACCATCGC-5'

                       ||x|||x|||x|||x

               5'-ATGCCTACAAAAACCAGTGC-3'

 

MFD455-AAT052-F TTCAGATGCCAGCTTCAAGA versus GATA89A11-F GCTTCTGAAAGCTTCTAGTTTACC

Matches = 8

Score = 7

GCNTCTGAA

est. tm =  4.0 oC

DeltaG @37 degrees =  -3.69 kcal/mole

 

                    5'-GCTTCTGAAAGCTTCTAGTTTACC-3'

                       ||x||||||

         3'-AGAACTTCGACCGTAGACTT-5'

 

GATA62F03N-F TTCCCAAAAGTTGCCATCTA versus GATA23F08-R TATATTTCTTGGGAAAGATAGATGG

Matches = 7

Score = 7

CCATCTA

est. tm =  0.3 oC

DeltaG @37 degrees =  -3.39 kcal/mole

 

                    3'-GGTAGATAGAAAGGGTTCTTTATAT-5'

                       |||||||

       5'-TTCCCAAAAGTTGCCATCTA-3'

 

GATA62F03N-F TTCCCAAAAGTTGCCATCTA versus GATA81C04-R TTAGCTGCTTCTGGGAAAGA

Matches = 12

Score = 7

NTNGCNNCTTNTGGGAA

est. tm =  12.9 oC

DeltaG @37 degrees =  -4.17 kcal/mole

 

                    5'-TTAGCTGCTTCTGGGAAAGA-3'

                       x|x||xx|||x||||||

                 3'-ATCTACCGTTGAAAACCCTT-5'

 

GATA62F03N-F TTCCCAAAAGTTGCCATCTA versus ATA59H06Z-R CTACTGCTGGGAATACCAGA

Matches = 10

Score = 7

CNACTNNTGGGAA

est. tm =  5.4 oC

DeltaG @37 degrees =  -3.33 kcal/mole

 

                    5'-CTACTGCTGGGAATACCAGA-3'

                       |x|||xx||||||

             3'-ATCTACCGTTGAAAACCCTT-5'

 

GATA62F03N-F TTCCCAAAAGTTGCCATCTA versus GATA116B01N-F ATGGCCTATTGTGGGATCTT

Matches = 12

Score = 7

ATGGCNNNTTNTGGGAN

est. tm =  24.5 oC

DeltaG @37 degrees =  -6.43 kcal/mole

 

                    5'-ATGGCCTATTGTGGGATCTT-3'

                       |||||xxx||x|||||x

                 3'-ATCTACCGTTGAAAACCCTT-5'

 

GATA62F03N-F TTCCCAAAAGTTGCCATCTA versus GATA8C04-F AGACCCTGTCTCAGATAGATGG

Matches = 7

Score = 7

CCATCTA

est. tm =  0.3 oC

DeltaG @37 degrees =  -3.39 kcal/mole

 

                    3'-GGTAGATAGACTCTGTCCCAGA-5'

                       |||||||

       5'-TTCCCAAAAGTTGCCATCTA-3'

 

GATA62F03N-F TTCCCAAAAGTTGCCATCTA versus ATA7D07-R ACGGGAGCTTTTGAGAAGTT

Matches = 13

Score = 9

ANGGNANCTTTTGNGAA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.51 kcal/mole

 

                    5'-ACGGGAGCTTTTGAGAAGTT-3'

                       |x||x|x||||||x|||

                 3'-ATCTACCGTTGAAAACCCTT-5'

 

AGAT142-F ATCCCATGCTCTGGTGCTAC versus GATA27-F AAGTGTTGCATCAGAGCCTC

Matches = 12

Score = 8

NANGCTCTGNTGCNAC

est. tm =  20.7 oC

DeltaG @37 degrees =  -5.65 kcal/mole

 

                    3'-CTCCGAGACTACGTTGTGAA-5'

                       x|x||||||x|||x||

                5'-ATCCCATGCTCTGGTGCTAC-3'

 

AGAT142-F ATCCCATGCTCTGGTGCTAC versus GATA175D03-R CAGGAGTATGGGATCACCAG

Matches = 11

Score = 8

CNNGAGNATGGGAT

est. tm =  9.8 oC

DeltaG @37 degrees =  -3.64 kcal/mole

 

                    5'-CAGGAGTATGGGATCACCAG-3'

                       |xx|||x|||||||

              3'-CATCGTGGTCTCGTACCCTA-5'

 

AGAT142-F ATCCCATGCTCTGGTGCTAC versus ATT070-R GGCAGTAGAGCCAGACCTTG

Matches = 12

Score = 8

CANGNTCTGGNNCTAC

est. tm =  6.9 oC

DeltaG @37 degrees =  -2.64 kcal/mole

 

                    3'-GTTCCAGACCGAGATGACGG-5'

                       ||x|x|||||xx||||

                5'-ATCCCATGCTCTGGTGCTAC-3'

 

GATA5E06-F GTGCACCTCTACACCCAGAC versus GATA12F04-F AGCCATCTGGGCTTAGAGAT

Matches = 12

Score = 8

ANCTCTANNCCCAGAN

est. tm =  20.2 oC

DeltaG @37 degrees =  -5.47 kcal/mole

 

                    3'-TAGAGATTCGGGTCTACCGA-5'

                       |x|||||xx||||||x

                5'-GTGCACCTCTACACCCAGAC-3'

 

GATA5E06-F GTGCACCTCTACACCCAGAC versus GATA175D03-F TGGAGTCTCTGGGTGAAGAG

Matches = 12

Score = 10

CTCTNCACCCAGAN

est. tm =  36.4 oC

DeltaG @37 degrees =  -9.21 kcal/mole

 

                    3'-GAGAAGTGGGTCTCTGAGGT-5'

                       ||||x||||||||x

              5'-GTGCACCTCTACACCCAGAC-3'

 

GATA5E06-F GTGCACCTCTACACCCAGAC versus GATA11E02N-R AGTGCTAGACTGGGAGAAGA

Matches = 10

Score = 7

TCTNCNCCCAGNC

est. tm =  less than zero

DeltaG @37 degrees =  -2.15 kcal/mole

 

                    3'-AGAAGAGGGTCAGATCGTGA-5'

                       |||x|x|||||x|

             5'-GTGCACCTCTACACCCAGAC-3'

 

GATA73H09N-R ATATTTCAGAACCGCAGCAG versus GATA89A11-F GCTTCTGAAAGCTTCTAGTTTACC

Matches = 10

Score = 7

GNTTCTGAAANNT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.57 kcal/mole

 

                    5'-GCTTCTGAAAGCTTCTAGTTTACC-3'

                       |x||||||||xx|

             3'-GACGACGCCAAGACTTTATA-5'

 

GATA81D05-R CTCAAGTCCCAAAAATGAGG versus GATA81C04-F TAGGATTTGAGACAAATGAAAGC

Matches = 9

Score = 7

TNGGANTTGAG

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.33 kcal/mole

 

                    5'-TAGGATTTGAGACAAATGAAAGC-3'

                       |x|||x|||||

           3'-GGAGTAAAAACCCTGAACTC-5'

 

GATG011-F CTCAAAGCCTGTGGAGGTCT versus GATA81C04-F TAGGATTTGAGACAAATGAAAGC

Matches = 9

Score = 7

NAGGNTTTGAG

est. tm =  11.1 oC

DeltaG @37 degrees =  -4.34 kcal/mole

 

                    5'-TAGGATTTGAGACAAATGAAAGC-3'

                       x|||x||||||

           3'-TCTGGAGGTGTCCGAAACTC-5'

 

ATA4E02-R CTGCTTTGGGTTTCACTTGT versus GATA81C04-F TAGGATTTGAGACAAATGAAAGC

Matches = 10

Score = 8

GNTTTCANTTGT

est. tm =  2.3 oC

DeltaG @37 degrees =  -2.56 kcal/mole

 

                    3'-CGAAAGTAAACAGAGTTTAGGAT-5'

                       |x|||||x||||

            5'-CTGCTTTGGGTTTCACTTGT-3'

 

sRA-R CCTTGTCAGCGTTTATTTGCC versus GATA81C04-F TAGGATTTGAGACAAATGAAAGC

Matches = 10

Score = 7

GCNTTNATTTGNC

est. tm =  less than zero

DeltaG @37 degrees =  -0.81 kcal/mole

 

                     3'-CGAAAGTAAACAGAGTTTAGGAT-5'

                        ||x||x|||||x|

             5'-CCTTGTCAGCGTTTATTTGCC-3'

 

GATA31-R TCATGTGAGCCAATTCCTCT versus GATA81C04-F TAGGATTTGAGACAAATGAAAGC

Matches = 13

Score = 7

NAGGANTTGNNNCANATGA

est. tm =  11.4 oC

DeltaG @37 degrees =  -3.24 kcal/mole

 

                    5'-TAGGATTTGAGACAAATGAAAGC-3'

                       x||||x|||xxx||x||||

                   3'-TCTCCTTAACCGAGTGTACT-5'

 

GATA11C08-R GGCTGCAGTTAGCTGTCATT versus GATA81C04-F TAGGATTTGAGACAAATGAAAGC

Matches = 8

Score = 7

GCTNTCATT

est. tm =  1.0 oC

DeltaG @37 degrees =  -3.25 kcal/mole

 

                    3'-CGAAAGTAAACAGAGTTTAGGAT-5'

                       |||x|||||

         5'-GGCTGCAGTTAGCTGTCATT-3'

 

GATA27-F AAGTGTTGCATCAGAGCCTC versus ATA59H06Z-F AAAGCGAGACTCTGTCTCAA

Matches = 11

Score = 7

TTGNNNCAGAGNCTC

est. tm =  5.9 oC

DeltaG @37 degrees =  -2.61 kcal/mole

 

                    3'-AACTCTGTCTCAGAGCGAAA-5'

                       |||xxx|||||x|||

               5'-AAGTGTTGCATCAGAGCCTC-3'

 

GATA27-F AAGTGTTGCATCAGAGCCTC versus ATA34E08N-F TGCACCACTTCATATTTCCA

Matches = 9

Score = 8

TGCANCACTT

est. tm =  14.4 oC

DeltaG @37 degrees =  -5.09 kcal/mole

 

                    5'-TGCACCACTTCATATTTCCA-3'

                       ||||x|||||

          3'-CTCCGAGACTACGTTGTGAA-5'

 

GATA27-F AAGTGTTGCATCAGAGCCTC versus ATA24A08-F GCAGCACTTGGCAAATACTC

Matches = 8

Score = 7

GCANCACTT

est. tm =  5.7 oC

DeltaG @37 degrees =  -3.98 kcal/mole

 

                    5'-GCAGCACTTGGCAAATACTC-3'

                       |||x|||||

         3'-CTCCGAGACTACGTTGTGAA-5'

 

GATA27-F AAGTGTTGCATCAGAGCCTC versus GATA27A12-F GTGGCTTTGGAGGAACACTA

Matches = 14

Score = 8

GNGGCTNTGNNGNAACACTN

est. tm =  19.0 oC

DeltaG @37 degrees =  -4.88 kcal/mole

 

                    5'-GTGGCTTTGGAGGAACACTA-3'

                       |x||||x||xx|x||||||x

                    3'-CTCCGAGACTACGTTGTGAA-5'

 

GATA27-F AAGTGTTGCATCAGAGCCTC versus GATA27A12-F GTGGCTTTGGAGGAACACTA

Matches = 14

Score = 8

NAGTGTTNCNNCANAGCCNC

est. tm =  17.4 oC

DeltaG @37 degrees =  -4.48 kcal/mole

 

                    3'-ATCACAAGGAGGTTTCGGTG-5'

                       x||||||x|xx||x||||x|

                    5'-AAGTGTTGCATCAGAGCCTC-3'

 

GATA27-F AAGTGTTGCATCAGAGCCTC versus GATA84E11-F TGTTGGAAGACTTCTCAGCC

Matches = 10

Score = 7

TGNTGNAANACTT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.77 kcal/mole

 

                    5'-TGTTGGAAGACTTCTCAGCC-3'

                       ||x||x||x||||

             3'-CTCCGAGACTACGTTGTGAA-5'

 

GATA27-F AAGTGTTGCATCAGAGCCTC versus GGAT3F08-F TTTTCCAGAAGCTCAGATCC

Matches = 10

Score = 7

GNATCNGAGCNTC

est. tm =  less than zero

DeltaG @37 degrees =  -1.54 kcal/mole

 

                    3'-CCTAGACTCGAAGACCTTTT-5'

                       |x|||x||||x||

             5'-AAGTGTTGCATCAGAGCCTC-3'

 

GATA68F07-F ATTGCTTCCCCTTTACCTGA versus GATA64G07-F TTTCCTAGTAGCTCAAGTAAAGAGG

Matches = 11

Score = 9

CCNCTTTACNTGA

est. tm =  9.7 oC

DeltaG @37 degrees =  -3.51 kcal/mole

 

                    3'-GGAGAAATGAACTCGATGATCCTTT-5'

                       ||x||||||x|||

             5'-ATTGCTTCCCCTTTACCTGA-3'

 

GATA73E11-F TTGGTGGTGTCATCCTCTTT versus GATA64G07-F TTTCCTAGTAGCTCAAGTAAAGAGG

Matches = 7

Score = 7

CCTCTTT

est. tm =  2.9 oC

DeltaG @37 degrees =  -3.78 kcal/mole

 

                    3'-GGAGAAATGAACTCGATGATCCTTT-5'

                       |||||||

       5'-TTGGTGGTGTCATCCTCTTT-3'

 

GGAA19H07-R GCCAAGCACTCTTTTCTTCA versus GATA64G07-F TTTCCTAGTAGCTCAAGTAAAGAGG

Matches = 10

Score = 7

NCTCTTTNCTTNA

est. tm =  less than zero

DeltaG @37 degrees =  -2.33 kcal/mole

 

                    3'-GGAGAAATGAACTCGATGATCCTTT-5'

                       x||||||x|||x|

             5'-GCCAAGCACTCTTTTCTTCA-3'

 

GATA88F09-F TACTTGGGATGCTAAGGTGG versus AGAT060-R TACCATGCCAAGGACATCAA

Matches = 11

Score = 8

TANCATNCCAAGNA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.32 kcal/mole

 

                    5'-TACCATGCCAAGGACATCAA-3'

                       ||x|||x|||||x|

              3'-GGTGGAATCGTAGGGTTCAT-5'

 

GATA88F09-F TACTTGGGATGCTAAGGTGG versus ATA12D05-R CACCTCAGCCTCTTTGGTAG

Matches = 13

Score = 7

CACCTNAGCNTCNNNNGTA

est. tm =  11.5 oC

DeltaG @37 degrees =  -3.16 kcal/mole

 

                    5'-CACCTCAGCCTCTTTGGTAG-3'

                       |||||x|||x||xxxx|||

                   3'-GGTGGAATCGTAGGGTTCAT-5'

 

GATA88F09-F TACTTGGGATGCTAAGGTGG versus ATA45G06-R GCCTCTTGTCATCCCAAGTA

Matches = 15

Score = 10

NCNNCTTNNCATCCCAAGTA

est. tm =  28.4 oC

DeltaG @37 degrees =  -6.76 kcal/mole

 

                    5'-GCCTCTTGTCATCCCAAGTA-3'

                       x|xx|||xx|||||||||||

                    3'-GGTGGAATCGTAGGGTTCAT-5'

 

GATA88F09-F TACTTGGGATGCTAAGGTGG versus ATA45G06-R GCCTCTTGTCATCCCAAGTA

Matches = 15

Score = 10

TACTTGGGATGNNAAGNNGN

est. tm =  29.7 oC

DeltaG @37 degrees =  -7.16 kcal/mole

 

                    3'-ATGAACCCTACTGTTCTCCG-5'

                       |||||||||||xx|||xx|x

                    5'-TACTTGGGATGCTAAGGTGG-3'

 

GATA88F09-F TACTTGGGATGCTAAGGTGG versus GATG011-R CCACCACGGTATCATCCAAT

Matches = 12

Score = 7

NTNGGATGNTANNGTGG

est. tm =  8.3 oC

DeltaG @37 degrees =  -3.06 kcal/mole

 

                    3'-TAACCTACTATGGCACCACC-5'

                       x|x|||||x||xx||||

                 5'-TACTTGGGATGCTAAGGTGG-3'

 

ATA31G11N-F GCTCCTGCGTACTGAGATA versus ATA29C07-R CCAGTACGCAGATGGTCCTA

Matches = 11

Score = 7

NCAGTACGCAGNNGN

est. tm =  31.4 oC

DeltaG @37 degrees =  -7.97 kcal/mole

 

                   5'-CCAGTACGCAGATGGTCCTA-3'

                      x||||||||||xx|x

               3'-ATAGAGTCATGCGTCCTCG-5'

 

ATA31G11N-F GCTCCTGCGTACTGAGATA versus ATA4E02-F TACTCAGGAGGCAGAGATGG

Matches = 9

Score = 7

TACNCAGGAGN

est. tm =  12.0 oC

DeltaG @37 degrees =  -4.70 kcal/mole

 

                   5'-TACTCAGGAGGCAGAGATGG-3'

                      |||x||||||x

           3'-ATAGAGTCATGCGTCCTCG-5'

 

ATA31G11N-F GCTCCTGCGTACTGAGATA versus ATTT030-F GGGTCCATTAGTTGAGTATGCAG

Matches = 11

Score = 7

CTGCNTACTNANNTA

est. tm =  less than zero

DeltaG @37 degrees =  -1.09 kcal/mole

 

                   3'-GACGTATGAGTTGATTACCTGGG-5'

                      ||||x||||x|xx||

               5'-GCTCCTGCGTACTGAGATA-3'

 

UT1772-F ATGAGGCTGGCAGACTCG versus GATA70E11-F GGCAGCCTAATAGTTCCGAT

Matches = 9

Score = 7

GNCAGCCTNAT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -3.07 kcal/mole

 

                  5'-GGCAGCCTAATAGTTCCGAT-3'

                     |x||||||x||

           3'-GCTCAGACGGTCGGAGTA-5'

 

GATA70E11-F GGCAGCCTAATAGTTCCGAT versus GATA188F04-F AACAGAACCAATAGGCTATCTATC

Matches = 14

Score = 8

ANCNGAACNANTAGGCTNNC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.99 kcal/mole

 

                    5'-AACAGAACCAATAGGCTATCTATC-3'

                       |x|x||||x|x||||||xx|

                    3'-TAGCCTTGATAATCCGACGG-5'

 

GATA70E11-F GGCAGCCTAATAGTTCCGAT versus GATA25A04-R GTGCATTATGGGGACCATTA

Matches = 10

Score = 7

TAATNGTNCCNAT

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                    3'-ATTACCAGGGGTATTACGTG-5'

                       ||||x||x||x||

             5'-GGCAGCCTAATAGTTCCGAT-3'

 

ATA5A04N-F TGCCACTGACTTCATTGCTA versus GATA52B03-F GAGCAATGATCTCATTTGCA

Matches = 15

Score = 10

NAGCAATGANNTCANTNGCA

est. tm =  30.4 oC

DeltaG @37 degrees =  -7.45 kcal/mole

 

                    5'-GAGCAATGATCTCATTTGCA-3'

                       x||||||||xx|||x|x|||

                    3'-ATCGTTACTTCAGTCACCGT-5'

 

ATA5A04N-F TGCCACTGACTTCATTGCTA versus GATA52B03-F GAGCAATGATCTCATTTGCA

Matches = 15

Score = 10

TGCNANTGANNTCATTGCTN

est. tm =  31.8 oC

DeltaG @37 degrees =  -7.85 kcal/mole

 

                    3'-ACGTTTACTCTAGTAACGAG-5'

                       |||x|x|||xx||||||||x

                    5'-TGCCACTGACTTCATTGCTA-3'

 

ATA5A04N-F TGCCACTGACTTCATTGCTA versus GATA193A07-R AGCCTGTGGCATCTTGTTAG

Matches = 9

Score = 7

AGNCNGTGGCA

est. tm =  3.1 oC

DeltaG @37 degrees =  -3.77 kcal/mole

 

                    5'-AGCCTGTGGCATCTTGTTAG-3'

                       ||x|x||||||

           3'-ATCGTTACTTCAGTCACCGT-5'

 

ATA24F10-F TCCTTTGTCAGTCATGCTCA versus GATA70E11-R AGTTTGCAGTGAGCATGGTT

Matches = 9

Score = 7

ANNCATGCTCA

est. tm =  9.2 oC

DeltaG @37 degrees =  -4.13 kcal/mole

 

                    3'-TTGGTACGAGTGACGTTTGA-5'

                       |xx||||||||

           5'-TCCTTTGTCAGTCATGCTCA-3'

 

ATA24F10-F TCCTTTGTCAGTCATGCTCA versus GATA21D04-F TAGGTTCTGGGCATGTCTGT

Matches = 10

Score = 7

NCAGNCATGCNCA

est. tm =  11.2 oC

DeltaG @37 degrees =  -4.34 kcal/mole

 

                    3'-TGTCTGTACGGGTCTTGGAT-5'

                       x|||x|||||x||

             5'-TCCTTTGTCAGTCATGCTCA-3'

 

GATA49C09N-F GCAACAGAGCAAGACTGTC versus ATA24F10-F TCCTTTGTCAGTCATGCTCA

Matches = 11

Score = 8

NGAGCANGACTGNC

est. tm =  20.6 oC

DeltaG @37 degrees =  -5.70 kcal/mole

 

                   3'-ACTCGTACTGACTGTTTCCT-5'

                      x|||||x|||||x|

              5'-GCAACAGAGCAAGACTGTC-3'

 

ATA24F10-F TCCTTTGTCAGTCATGCTCA versus GATA165G02N-R CTGTCACAGGACTTTTCAGCC

Matches = 9

Score = 7

CTGNCANAGGA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.74 kcal/mole

 

                    5'-CTGTCACAGGACTTTTCAGCC-3'

                       |||x||x||||

           3'-ACTCGTACTGACTGTTTCCT-5'

 

UT721-F TTGGTCTCCTCTGATGGGT versus GATA87G01-F CAGTGGACACTAAACACAATCC

Matches = 10

Score = 7

CAGNGGANACNAA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.50 kcal/mole

 

                   5'-CAGTGGACACTAAACACAATCC-3'

                      |||x|||x||x||

             3'-TGGGTAGTCTCCTCTGGTT-5'

 

GATA31D10-F GTGGGACCTTGTGATTGTGT versus GATA87G01-F CAGTGGACACTAAACACAATCC

Matches = 8

Score = 7

NGATTGTGT

est. tm =  14.3 oC

DeltaG @37 degrees =  -5.07 kcal/mole

 

                    3'-CCTAACACAAATCACAGGTGAC-5'

                       x||||||||

         5'-GTGGGACCTTGTGATTGTGT-3'

 

ATA33B03Z-R TAGTATCCACTTCCTGGGGT versus GATA87G01-F CAGTGGACACTAAACACAATCC

Matches = 10

Score = 8

NAGTGGANACTA

est. tm =  18.4 oC

DeltaG @37 degrees =  -5.47 kcal/mole

 

                    5'-CAGTGGACACTAAACACAATCC-3'

                       x||||||x||||

            3'-TGGGGTCCTTCACCTATGAT-5'

 

GATA63F09-R CTGTCTAGTGTCCTGAGCTGC versus GATA87G01-F CAGTGGACACTAAACACAATCC

Matches = 12

Score = 7

CNNNGGACACTANACAN

est. tm =  19.0 oC

DeltaG @37 degrees =  -4.95 kcal/mole

 

                     5'-CAGTGGACACTAAACACAATCC-3'

                        |xxx||||||||x|||x

                 3'-CGTCGAGTCCTGTGATCTGTC-5'

 

GATA145D10N-R TTGAGCCCAGAAGTTTGAG versus GATA115E01N-F CCTCCTAGCTCCTCAAGCTT

Matches = 8

Score = 7

AAGNTTGAG

est. tm =  less than zero

DeltaG @37 degrees =  -2.45 kcal/mole

 

                   3'-TTCGAACTCCTCGATCCTCC-5'

                      |||x|||||

         5'-TTGAGCCCAGAAGTTTGAG-3'

 

GATA115E01N-F CCTCCTAGCTCCTCAAGCTT versus MFD455-AAT052-R GATGAGCCAGGAAGCCATAA

Matches = 11

Score = 8

GANGAGCNAGGANG

est. tm =  7.9 oC

DeltaG @37 degrees =  -3.50 kcal/mole

 

                    5'-GATGAGCCAGGAAGCCATAA-3'

                       ||x||||x||||x|

              3'-TTCGAACTCCTCGATCCTCC-5'

 

GATA72G11N-R CAAAATTAGCTGGGTATTGTCTAG versus GATA64A09-F CCCTAGCTAATGTTAAAATATCACG

Matches = 11

Score = 7

NCCNAGCTAATNTTN

est. tm =  15.5 oC

DeltaG @37 degrees =  -4.71 kcal/mole

 

                        5'-CCCTAGCTAATGTTAAAATATCACG-3'

                           x||x|||||||x||x

               3'-GATCTGTTATGGGTCGATTAAAAC-5'

 

GATA64A09-F CCCTAGCTAATGTTAAAATATCACG versus GATA124E07-R ATATTCTTATATTCCATATGGCACA

Matches = 14

Score = 7

ATATTNTNANATTNNNTANGG

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                         5'-ATATTCTTATATTCCATATGGCACA-3'

                            |||||x|x|x|||xxx||x||

                     3'-GCACTATAAAATTGTAATCGATCCC-5'

 

GATA64A09-F CCCTAGCTAATGTTAAAATATCACG versus GATA138B05-F TGGAAATCTTAAGAATATCTAGGTG

Matches = 16

Score = 8

NNGANATNTTAANANTANCTAGGN

est. tm =  4.8 oC

DeltaG @37 degrees =  -0.85 kcal/mole

 

                         5'-TGGAAATCTTAAGAATATCTAGGTG-3'

                            xx||x||x||||x|x||x|||||x

                        3'-GCACTATAAAATTGTAATCGATCCC-5'

 

GATA64D03-F ACATTTCCAAGGGCTACACA versus GATA64A09-F CCCTAGCTAATGTTAAAATATCACG

Matches = 10

Score = 7

CCCTNGNNAATGT

est. tm =  2.7 oC

DeltaG @37 degrees =  -2.89 kcal/mole

 

                    5'-CCCTAGCTAATGTTAAAATATCACG-3'

                       ||||x|xx|||||

             3'-ACACATCGGGAACCTTTACA-5'

 

GATA48D07-R TGTAACAAGGGCTATGGGAA versus GATA64A09-F CCCTAGCTAATGTTAAAATATCACG

Matches = 12

Score = 7

CCNTAGCNNNTGTTANA

est. tm =  2.0 oC

DeltaG @37 degrees =  -1.68 kcal/mole

 

                    5'-CCCTAGCTAATGTTAAAATATCACG-3'

                       ||x||||xxx|||||x|

                 3'-AAGGGTATCGGGAACAATGT-5'

 

ATA29C03-F GACTCTGCTAAGCACATTTCC versus GATA28D01-R TTTGAAGATCTGTGAAATGTGC

Matches = 9

Score = 8

GCACATTTCN

est. tm =  26.5 oC

DeltaG @37 degrees =  -7.04 kcal/mole

 

                     3'-CGTGTAAAGTGTCTAGAAGTTT-5'

                        |||||||||x

          5'-GACTCTGCTAAGCACATTTCC-3'

 

ATA29C03-F GACTCTGCTAAGCACATTTCC versus ATA21A03-F TGCTTACCATAATCAACATTGA

Matches = 11

Score = 8

TGCTTANCANANTC

est. tm =  0.9 oC

DeltaG @37 degrees =  -2.23 kcal/mole

 

                     5'-TGCTTACCATAATCAACATTGA-3'

                        ||||||x||x|x||

              3'-CCTTTACACGAATCGTCTCAG-5'

 

UT1772-F ATGAGGCTGGCAGACTCG versus ATA29C03-F GACTCTGCTAAGCACATTTCC

Matches = 12

Score = 7

GANTCTGCNANNCNCAT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.16 kcal/mole

 

                  5'-GACTCTGCTAAGCACATTTCC-3'

                     ||x|||||x|xx|x|||

                 3'-GCTCAGACGGTCGGAGTA-5'

 

ATGT006Z-R CTGCACAGCAGAGTGAGACC versus ATA29C03-F GACTCTGCTAAGCACATTTCC

Matches = 11

Score = 7

NACTCTGCTNNGCAN

est. tm =  33.8 oC

DeltaG @37 degrees =  -8.59 kcal/mole

 

                    5'-GACTCTGCTAAGCACATTTCC-3'

                       x||||||||xx|||x

               3'-CCAGAGTGAGACGACACGTC-5'

 

GGAA23C05N-F GGAATAAGTGCAGTGCTTGG versus ATA31G11N-R GCACAGTACTTCTCCCTCCA

Matches = 12

Score = 8

GCACNGNACTTNTNCC

est. tm =  4.5 oC

DeltaG @37 degrees =  -2.69 kcal/mole

 

                    5'-GCACAGTACTTCTCCCTCCA-3'

                       ||||x|x||||x|x||

                3'-GGTTCGTGACGTGAATAAGG-5'

 

GGAA23C05N-F GGAATAAGTGCAGTGCTTGG versus GAAT1A4N-R CCCAAGGGCAGAAATTATTA

Matches = 13

Score = 7

NAATAANTNCNGNNCTTGG

est. tm =  less than zero

DeltaG @37 degrees =  -0.37 kcal/mole

 

                    3'-ATTATTAAAGACGGGAACCC-5'

                       x|||||x|x|x|xx|||||

                   5'-GGAATAAGTGCAGTGCTTGG-3'

 

GGAA17G05-F GGGTGACAGAGCAAAATTCT versus GATA7C06-R TAGGATTTTACTATATTCCCCAGG

Matches = 14

Score = 8

AGNATTTTNCTNTNTNNCCC

est. tm =  less than zero

DeltaG @37 degrees =  -0.12 kcal/mole

 

                    5'-TAGGATTTTACTATATTCCCCAGG-3'

                        ||x|||||x||x|x|xx|||

                     3'-TCTTAAAACGAGACAGTGGG-5'

 

GATA23F06-F TACATGGCAGCAGGCATATA versus GATA23F06-F TACATGGCAGCAGGCATATA

Matches = 14

Score = 8

TANATGNCNGCNGNCATNTA

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                    5'-TACATGGCAGCAGGCATATA-3'

                       ||x|||x|x||x|x|||x||

                    3'-ATATACGGACGACGGTACAT-5'

 

GATA23F06-F TACATGGCAGCAGGCATATA versus GATA23F06-F TACATGGCAGCAGGCATATA

Matches = 14

Score = 8

TANATGNCNGCNGNCATNTA

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                    3'-ATATACGGACGACGGTACAT-5'

                       ||x|||x|x||x|x|||x||

                    5'-TACATGGCAGCAGGCATATA-3'

 

GATA23F06-F TACATGGCAGCAGGCATATA versus ATCC001-F TGCCCTGTATTCTGTGTAGCA

Matches = 8

Score = 7

TGCCNTGTA

est. tm =  4.4 oC

DeltaG @37 degrees =  -4.04 kcal/mole

 

                    5'-TGCCCTGTATTCTGTGTAGCA-3'

                       ||||x||||

         3'-ATATACGGACGACGGTACAT-5'

 

ATA34E08N-F TGCACCACTTCATATTTCCA versus GATA23F05-F ATGGTATTAGGAGATGGGGC

Matches = 13

Score = 7

GCNCCANNTCNTANTNCCA

est. tm =  less than zero

DeltaG @37 degrees =  -0.97 kcal/mole

 

                    3'-CGGGGTAGAGGATTATGGTA-5'

                       ||x|||xx||x||x|x|||

                   5'-TGCACCACTTCATATTTCCA-3'

 

ATA34E08N-F TGCACCACTTCATATTTCCA versus AGAT144-R ATCCCAGATGGAAACATGGA

Matches = 10

Score = 8

TNCATNTTTCCA

est. tm =  1.2 oC

DeltaG @37 degrees =  -2.73 kcal/mole

 

                    3'-AGGTACAAAGGTAGACCCTA-5'

                       |x|||x||||||

            5'-TGCACCACTTCATATTTCCA-3'

 

GATA30D09N-R AGCCTCTGTGCAGATCCTC versus ATA1B07-F GGACAGATGCTTCCAGAAAA

Matches = 9

Score = 7

GNACAGANGCT

est. tm =  0.9 oC

DeltaG @37 degrees =  -3.14 kcal/mole

 

                   5'-GGACAGATGCTTCCAGAAAA-3'

                      |x|||||x|||

           3'-CTCCTAGACGTGTCTCCGA-5'

 

ATA1B07-F GGACAGATGCTTCCAGAAAA versus GATA11C06N-R AGCGTTTGTGTGTGCATCTGT

Matches = 13

Score = 8

ACAGATGCNNNCANAAAN

est. tm =  23.8 oC

DeltaG @37 degrees =  -5.87 kcal/mole

 

                    3'-TGTCTACGTGTGTGTTTGCGA-5'

                       ||||||||xxx||x|||x

                  5'-GGACAGATGCTTCCAGAAAA-3'

 

ATA9B04N-F ACCCGTAATCTGTCTCTCCC versus GATA23F08-R TATATTTCTTGGGAAAGATAGATGG

Matches = 11

Score = 7

NNATCTNTCTNTCCC

est. tm =  13.3 oC

DeltaG @37 degrees =  -4.26 kcal/mole

 

                    3'-GGTAGATAGAAAGGGTTCTTTATAT-5'

                       xx||||x|||x||||

               5'-ACCCGTAATCTGTCTCTCCC-3'

 

ATA9B04N-F ACCCGTAATCTGTCTCTCCC versus GGAA22G01-F ACAAAAGCTGGCAAAGACAG

Matches = 9

Score = 7

CTGTCTNTNCC

est. tm =  less than zero

DeltaG @37 degrees =  -2.80 kcal/mole

 

                    3'-GACAGAAACGGTCGAAAACA-5'

                       ||||||x|x||

           5'-ACCCGTAATCTGTCTCTCCC-3'

 

ATA9B04N-F ACCCGTAATCTGTCTCTCCC versus GATA63A03N-F GTGGATAGACACATGACAGATAGG

Matches = 14

Score = 8

NGGANAGACANATNACNGNT

est. tm =  2.8 oC

DeltaG @37 degrees =  -1.93 kcal/mole

 

                    5'-GTGGATAGACACATGACAGATAGG-3'

                        x|||x|||||x||x||x|x|

                     3'-CCCTCTCTGTCTAATGCCCA-5'

 

ATA9B04N-F ACCCGTAATCTGTCTCTCCC versus MFD466-TTA001-R GCCTGGGCAAAGAGAGAGTA

Matches = 11

Score = 7

TANTCTNTCTNTNCC

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                    3'-ATGAGAGAGAAACGGGTCCG-5'

                       ||x|||x|||x|x||

               5'-ACCCGTAATCTGTCTCTCCC-3'

 

GATA12F04-F AGCCATCTGGGCTTAGAGAT versus TTTA028-R GGCACCTTTAATCCCAGCTA

Matches = 12

Score = 7

NANCTGGGNTTANAGNT

est. tm =  less than zero

DeltaG @37 degrees =  -1.49 kcal/mole

 

                    3'-ATCGACCCTAATTTCCACGG-5'

                       x|x|||||x|||x||x|

                 5'-AGCCATCTGGGCTTAGAGAT-3'

 

GATA12F04-F AGCCATCTGGGCTTAGAGAT versus GATA134B03-R GCTGCTTCCCTAAACCTAGA

Matches = 11

Score = 7

TCTNGGNTTAGNGAN

est. tm =  less than zero

DeltaG @37 degrees =  -2.19 kcal/mole

 

                    3'-AGATCCAAATCCCTTCGTCG-5'

                       |||x||x||||x||x

               5'-AGCCATCTGGGCTTAGAGAT-3'

 

GATA12F04-F AGCCATCTGGGCTTAGAGAT versus AGAT144-R ATCCCAGATGGAAACATGGA

Matches = 10

Score = 7

ANCCCAGATGGNN

est. tm =  22.6 oC

DeltaG @37 degrees =  -6.39 kcal/mole

 

                    5'-ATCCCAGATGGAAACATGGA-3'

                       |x|||||||||xx

             3'-TAGAGATTCGGGTCTACCGA-5'

 

GATA90D07N-F CTGAGGTGGGAGGATCAGTT versus AATA058-F CCCATCTCAGGAAGGGAAAC

Matches = 9

Score = 8

CCCANCTCAG

est. tm =  16.1 oC

DeltaG @37 degrees =  -5.31 kcal/mole

 

                    5'-CCCATCTCAGGAAGGGAAAC-3'

                       ||||x|||||

          3'-TTGACTAGGAGGGTGGAGTC-5'

 

GATA90D07N-F CTGAGGTGGGAGGATCAGTT versus ATA12D05-R CACCTCAGCCTCTTTGGTAG

Matches = 8

Score = 8

CACCTCAG

est. tm =  19.2 oC

DeltaG @37 degrees =  -5.90 kcal/mole

 

                    5'-CACCTCAGCCTCTTTGGTAG-3'

                       ||||||||

        3'-TTGACTAGGAGGGTGGAGTC-5'

 

GATA12F04-R TATAGGCACATGCCACTGTG versus GATA28D01-F AGTAGAGAACAAAACACTGTGGC

Matches = 8

Score = 7

GCCACNGTG

est. tm =  12.6 oC

DeltaG @37 degrees =  -5.02 kcal/mole

 

                    3'-CGGTGTCACAAAACAAGAGATGA-5'

                       |||||x|||

         5'-TATAGGCACATGCCACTGTG-3'

 

GATA28D01-F AGTAGAGAACAAAACACTGTGGC versus GGAA3C07-R TTCTCTACATATTTACTGCCAACA

Matches = 8

Score = 7

TTCTCTACN

est. tm =  12.0 oC

DeltaG @37 degrees =  -4.70 kcal/mole

 

                       5'-TTCTCTACATATTTACTGCCAACA-3'

                          ||||||||x

         3'-CGGTGTCACAAAACAAGAGATGA-5'

 

GATA23E06-F AGCCATCAACTAGCTTTCCC versus GATA23F08-R TATATTTCTTGGGAAAGATAGATGG

Matches = 12

Score = 9

NCANCTANCTTTCCC

est. tm =  19.7 oC

DeltaG @37 degrees =  -5.24 kcal/mole

 

                    3'-GGTAGATAGAAAGGGTTCTTTATAT-5'

                       x||x|||x|||||||

               5'-AGCCATCAACTAGCTTTCCC-3'

 

GATA23E06-F AGCCATCAACTAGCTTTCCC versus GATA21C12-R CCCATTGGAAACAGAGTTGA

Matches = 11

Score = 7

TCAACTNNNTTTCCN

est. tm =  19.6 oC

DeltaG @37 degrees =  -5.25 kcal/mole

 

                    3'-AGTTGAGACAAAGGTTACCC-5'

                       ||||||xxx|||||x

               5'-AGCCATCAACTAGCTTTCCC-3'

 

GATA117D01N-F TATCCTATGGAAGCCATCA versus AAT269-F GTCGAATGATGGCTTTCCTG

Matches = 11

Score = 8

NANGNAAGCCATCA

est. tm =  18.0 oC

DeltaG @37 degrees =  -5.30 kcal/mole

 

                   3'-GTCCTTTCGGTAGTAAGCTG-5'

                      x|x|x|||||||||

              5'-TATCCTATGGAAGCCATCA-3'

 

GATA117D01N-F TATCCTATGGAAGCCATCA versus ATA22E01-R AGGACTGTAGGATTCCAAAGG

Matches = 12

Score = 8

CCTNTGGAANCCNNCA

est. tm =  11.5 oC

DeltaG @37 degrees =  -3.92 kcal/mole

 

                   3'-GGAAACCTTAGGATGTCAGGA-5'

                      |||x|||||x||xx||

                5'-TATCCTATGGAAGCCATCA-3'

 

GATA117D01N-F TATCCTATGGAAGCCATCA versus GATA6A05-F CTGCCAGAGAATAGGGTGAA

Matches = 10

Score = 7

CTNCCANAGNATA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.47 kcal/mole

 

                   5'-CTGCCAGAGAATAGGGTGAA-3'

                      ||x|||x||x|||

             3'-ACTACCGAAGGTATCCTAT-5'

 

AAC040Z-F ACACGGTGAAACCCTGTCTC versus AAC040Z-F ACACGGTGAAACCCTGTCTC

Matches = 12

Score = 7

ACANGGTNNNACCNTGT

est. tm =  9.6 oC

DeltaG @37 degrees =  -3.55 kcal/mole

 

                    5'-ACACGGTGAAACCCTGTCTC-3'

                       |||x|||xxx|||x|||

                 3'-CTCTGTCCCAAAGTGGCACA-5'

 

AAC040Z-F ACACGGTGAAACCCTGTCTC versus GATA64A09-R CAGAGTGAGACCCTGTTTCA

Matches = 11

Score = 8

TGAAACNNNGTCTC

est. tm =  16.8 oC

DeltaG @37 degrees =  -4.49 kcal/mole

 

                    3'-ACTTTGTCCCAGAGTGAGAC-5'

                       ||||||xxx|||||

              5'-ACACGGTGAAACCCTGTCTC-3'

 

GGAA3A07Z-F TCCCATGCCAAAATTCTTAG versus UT5029-F GTTTGGTATTGGAGTTTTCAG

Matches = 10

Score = 7

NTTTGGNATNGGA

est. tm =  5.4 oC

DeltaG @37 degrees =  -3.45 kcal/mole

 

                    5'-GTTTGGTATTGGAGTTTTCAG-3'

                       x|||||x||x|||

             3'-GATTCTTAAAACCGTACCCT-5'

 

GATA66B04-F ATGCCCTCTTCTGTCTCTCC versus GATA26D02N-F ATAGACAGGATGGATAGATAGACG

Matches = 10

Score = 7

CNTCTNTCTNTCC

est. tm =  less than zero

DeltaG @37 degrees =  -1.55 kcal/mole

 

                    3'-GCAGATAGATAGGTAGGACAGATA-5'

                       |x|||x|||x|||

             5'-ATGCCCTCTTCTGTCTCTCC-3'

 

GATA124E07-F CCAGCCTGGCTGTTAGAGTA versus GATA26D02N-F ATAGACAGGATGGATAGATAGACG

Matches = 10

Score = 7

ANAGNCAGGNTGG

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.29 kcal/mole

 

                    5'-ATAGACAGGATGGATAGATAGACG-3'

                       |x||x||||x|||

             3'-ATGAGATTGTCGGTCCGACC-5'

 

ATA5A09N-R CACCCCACGTCTAGCTATAG versus GATA26D02N-F ATAGACAGGATGGATAGATAGACG

Matches = 10

Score = 7

CGTCTANCTATNN

est. tm =  13.4 oC

DeltaG @37 degrees =  -4.14 kcal/mole

 

                    3'-GCAGATAGATAGGTAGGACAGATA-5'

                       ||||||x||||xx

             5'-CACCCCACGTCTAGCTATAG-3'

 

GATA26D02N-F ATAGACAGGATGGATAGATAGACG versus GATA188F04-F AACAGAACCAATAGGCTATCTATC

Matches = 10

Score = 8

GATAGATAGNCN

est. tm =  9.0 oC

DeltaG @37 degrees =  -3.39 kcal/mole

 

                        3'-CTATCTATCGGATAACCAAGACAA-5'

                           |||||||||x|x

            5'-ATAGACAGGATGGATAGATAGACG-3'

 

GATA26D02N-F ATAGACAGGATGGATAGATAGACG versus GATA22G05-R AAAACAAATATGGCTCTATCTATCG

Matches = 10

Score = 7

NGATAGATAGANN

est. tm =  18.3 oC

DeltaG @37 degrees =  -4.99 kcal/mole

 

                        3'-GCTATCTATCTCGGTATAAACAAAA-5'

                           x||||||||||xx

             5'-ATAGACAGGATGGATAGATAGACG-3'

 

GATA26D02N-F ATAGACAGGATGGATAGATAGACG versus GATA129D11N-R TAGTACTCTACCATCCATCTATCCC

Matches = 11

Score = 8

NGGATAGATNGANG

est. tm =  8.8 oC

DeltaG @37 degrees =  -3.29 kcal/mole

 

                        3'-CCCTATCTACCTACCATCTCATGAT-5'

                           x||||||||x||x|

              5'-ATAGACAGGATGGATAGATAGACG-3'

 

GATA26D02N-F ATAGACAGGATGGATAGATAGACG versus AGAT120-R TTGTCTGTCATCTATCTACCCATC

Matches = 14

Score = 12

GATGGNTAGATAGANG

est. tm =  23.4 oC

DeltaG @37 degrees =  -5.50 kcal/mole

 

                        3'-CTACCCATCTATCTACTGTCTGTT-5'

                           |||||x||||||||x|

                5'-ATAGACAGGATGGATAGATAGACG-3'

 

GATA21F03-R CCTGCCTGCCTATCTATCTG versus GATA26D02N-F ATAGACAGGATGGATAGATAGACG

Matches = 10

Score = 7

ATAGNCAGGNNGG

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.30 kcal/mole

 

                    5'-ATAGACAGGATGGATAGATAGACG-3'

                       ||||x||||xx||

             3'-GTCTATCTATCCGTCCGTCC-5'

 

GATA85A04N-F CCTCACACAATGTTGTAAGGG versus GATA85A04N-F CCTCACACAATGTTGTAAGGG

Matches = 14

Score = 8

CCTNACANNATNNTGTNAGG

est. tm =  11.0 oC

DeltaG @37 degrees =  -2.96 kcal/mole

 

                     5'-CCTCACACAATGTTGTAAGGG-3'

                        |||x|||xx||xx|||x|||

                    3'-GGGAATGTTGTAACACACTCC-5'

 

GATA104-F CCTCCATAATTGTGTGAGCC versus GATA85A04N-F CCTCACACAATGTTGTAAGGG

Matches = 14

Score = 9

NCTCACACAATNNTGNANG

est. tm =  20.6 oC

DeltaG @37 degrees =  -4.92 kcal/mole

 

                    5'-CCTCACACAATGTTGTAAGGG-3'

                       x||||||||||xx||x|x|

                   3'-CCGAGTGTGTTAATACCTCC-5'

 

TCTA015-F GAATTGTGTGAGCCGGTTTC versus GATA85A04N-F CCTCACACAATGTTGTAAGGG

Matches = 10

Score = 7

NCTCACACAATNN

est. tm =  28.5 oC

DeltaG @37 degrees =  -7.32 kcal/mole

 

                    5'-CCTCACACAATGTTGTAAGGG-3'

                       x||||||||||xx

             3'-CTTTGGCCGAGTGTGTTAAG-5'

 

ATA19H08-R GCATTGTGGGATGAGGTATG versus GATA85A04N-F CCTCACACAATGTTGTAAGGG

Matches = 10

Score = 7

CNTCNCACAATGN

est. tm =  8.2 oC

DeltaG @37 degrees =  -3.61 kcal/mole

 

                    5'-CCTCACACAATGTTGTAAGGG-3'

                       |x||x|||||||x

             3'-GTATGGAGTAGGGTGTTACG-5'

 

GATA85A04N-F CCTCACACAATGTTGTAAGGG versus AAT107-R CATTCTGAGAGGAAGGCAGAA

Matches = 10

Score = 8

CATTNTGNGAGG

est. tm =  5.4 oC

DeltaG @37 degrees =  -3.12 kcal/mole

 

                     5'-CATTCTGAGAGGAAGGCAGAA-3'

                        ||||x||x||||

            3'-GGGAATGTTGTAACACACTCC-5'

 

GATA32F05-F ATTTCACGTACTTGGCAAGC versus AGAT060-R TACCATGCCAAGGACATCAA

Matches = 13

Score = 8

TTNANGTNCTTGGCANGN

est. tm =  12.8 oC

DeltaG @37 degrees =  -4.06 kcal/mole

 

                    3'-AACTACAGGAACCGTACCAT-5'

                       ||x|x||x|||||||x|x

                  5'-ATTTCACGTACTTGGCAAGC-3'

 

GATA32F05-F ATTTCACGTACTTGGCAAGC versus GATA68F07-F ATTGCTTCCCCTTTACCTGA

Matches = 12

Score = 7

TCANGTANNNGGNAAGC

est. tm =  5.4 oC

DeltaG @37 degrees =  -2.40 kcal/mole

 

                    3'-AGTCCATTTCCCCTTCGTTA-5'

                       |||x|||xxx||x||||

                 5'-ATTTCACGTACTTGGCAAGC-3'

 

GATA32F05-F ATTTCACGTACTTGGCAAGC versus GATA89G08Z-R AAGCATGTGAAAGGCATTGT

Matches = 10

Score = 7

AAGNANGTGAAAN

est. tm =  4.3 oC

DeltaG @37 degrees =  -3.07 kcal/mole

 

                    5'-AAGCATGTGAAAGGCATTGT-3'

                       |||x|x||||||x

             3'-CGAACGGTTCATGCACTTTA-5'

 

AAAAT002-F AGGTTGCAGTGAGCCAAGAT versus ATA10H11-F GGTGACAGAGACTCTTGTCTCA

Matches = 9

Score = 7

TGAGNCAAGAN

est. tm =  12.0 oC

DeltaG @37 degrees =  -4.61 kcal/mole

 

                    3'-ACTCTGTTCTCAGAGACAGTGG-5'

                       ||||x|||||x

           5'-AGGTTGCAGTGAGCCAAGAT-3'

 

AAAAT002-F AGGTTGCAGTGAGCCAAGAT versus GATA70E01-F CCCACTACAACTTCAGGTAGA

Matches = 10

Score = 7

CNCACTNCAACNT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.27 kcal/mole

 

                    5'-CCCACTACAACTTCAGGTAGA-3'

                       |x||||x||||x|

             3'-TAGAACCGAGTGACGTTGGA-5'

 

AAAAT002-F AGGTTGCAGTGAGCCAAGAT versus GATA22F11NZ-R TCTCTCTTGCTCCACTGCAA

Matches = 13

Score = 9

TTGCAGTGNNNCAAGAN

est. tm =  33.7 oC

DeltaG @37 degrees =  -8.43 kcal/mole

 

                    3'-AACGTCACCTCGTTCTCTCT-5'

                       ||||||||xxx|||||x

                 5'-AGGTTGCAGTGAGCCAAGAT-3'

 

AAAAT002-F AGGTTGCAGTGAGCCAAGAT versus AATA036-R ACTGACTGCAGCCTTGACCT

Matches = 11

Score = 8

NCTNACTGCANCCT

est. tm =  21.8 oC

DeltaG @37 degrees =  -6.13 kcal/mole

 

                    5'-ACTGACTGCAGCCTTGACCT-3'

                       x||x||||||x|||

              3'-TAGAACCGAGTGACGTTGGA-5'

 

ATA5A09N-F GTGGGCCACAAAGCAAGACT versus ATA5A09N-F GTGGGCCACAAAGCAAGACT

Matches = 8

Score = 7

GTGGNCCAC

est. tm =  9.7 oC

DeltaG @37 degrees =  -4.61 kcal/mole

 

                    5'-GTGGGCCACAAAGCAAGACT-3'

                       ||||x||||

         3'-TCAGAACGAAACACCGGGTG-5'

 

ATA5A09N-F GTGGGCCACAAAGCAAGACT versus GATA178G09-R TCTTAGTTTATGGGCCACCA

Matches = 14

Score = 10

TCTTNNTTTNTGGNCCAC

est. tm =  20.4 oC

DeltaG @37 degrees =  -5.01 kcal/mole

 

                    5'-TCTTAGTTTATGGGCCACCA-3'

                       ||||xx|||x|||x||||

                  3'-TCAGAACGAAACACCGGGTG-5'

 

ATA5A09N-F GTGGGCCACAAAGCAAGACT versus GATA30G01-R AATGAGGTCTTACTTTGTTGCC

Matches = 14

Score = 11

GGCNACAAAGNAAGACN

est. tm =  35.1 oC

DeltaG @37 degrees =  -8.81 kcal/mole

 

                    3'-CCGTTGTTTCATTCTGGAGTAA-5'

                       |||x||||||x|||||x

                 5'-GTGGGCCACAAAGCAAGACT-3'

 

GATA64F08-F AGGGATCTTCAGAGAAACAGG versus GATA124C08N-R ATGTGTGTGCATCTGTCTCTCTCA

Matches = 10

Score = 7

TNAGAGANACAGN

est. tm =  8.4 oC

DeltaG @37 degrees =  -3.92 kcal/mole

 

                     3'-ACTCTCTCTGTCTACGTGTGTGTA-5'

                        |x|||||x||||x

             5'-AGGGATCTTCAGAGAAACAGG-3'

 

GATA64F08-F AGGGATCTTCAGAGAAACAGG versus GATA11A11-R TCTGGAAAACCCTAATTCTCC

Matches = 10

Score = 7

TCTGNANANCCCT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.09 kcal/mole

 

                     5'-TCTGGAAAACCCTAATTCTCC-3'

                        ||||x|x|x||||

             3'-GGACAAAGAGACTTCTAGGGA-5'

 

GATA64A09-R CAGAGTGAGACCCTGTTTCA versus GATA64F08-F AGGGATCTTCAGAGAAACAGG

Matches = 8

Score = 7

CCTGTTTCN

est. tm =  18.9 oC

DeltaG @37 degrees =  -5.87 kcal/mole

 

                    3'-GGACAAAGAGACTTCTAGGGA-5'

                       ||||||||x

         5'-CAGAGTGAGACCCTGTTTCA-3'

 

GATA64F08-F AGGGATCTTCAGAGAAACAGG versus GATA28D01-R TTTGAAGATCTGTGAAATGTGC

Matches = 10

Score = 7

TNTGAAGATCNNT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.66 kcal/mole

 

                     5'-TTTGAAGATCTGTGAAATGTGC-3'

                        |x||||||||xx|

             3'-GGACAAAGAGACTTCTAGGGA-5'

 

GATA84E11-R CACACATGCTGTGTCTCTCA versus GATA64F08-F AGGGATCTTCAGAGAAACAGG

Matches = 10

Score = 7

NCTGTNTCTCTNA

est. tm =  6.3 oC

DeltaG @37 degrees =  -3.52 kcal/mole

 

                    3'-GGACAAAGAGACTTCTAGGGA-5'

                       x||||x|||||x|

             5'-CACACATGCTGTGTCTCTCA-3'

 

GATA137H02N-R TCTGAGGATTCCTCCTTCCT versus GATA64F08-F AGGGATCTTCAGAGAAACAGG

Matches = 11

Score = 9

AGGNATCNTCAGA

est. tm =  11.7 oC

DeltaG @37 degrees =  -4.16 kcal/mole

 

                    5'-AGGGATCTTCAGAGAAACAGG-3'

                       |||x|||x|||||

             3'-TCCTTCCTCCTTAGGAGTCT-5'

 

AGAT060-F CATGTAAGATTTTGCTGCCAGA versus GATA43H03N-F GGCATAAAAATAGTACAGCAAGC

Matches = 13

Score = 7

GGCANNAAAATNNTACANN

est. tm =  19.5 oC

DeltaG @37 degrees =  -4.63 kcal/mole

 

                      5'-GGCATAAAAATAGTACAGCAAGC-3'

                         ||||xx|||||xx||||xx

                   3'-AGACCGTCGTTTTAGAATGTAC-5'

 

GATA43H03N-F GGCATAAAAATAGTACAGCAAGC versus GATA72E05-R AATCAGTGCTTTCTGTACTATTGG

Matches = 14

Score = 11

NNAATAGTACAGNAAGC

est. tm =  31.3 oC

DeltaG @37 degrees =  -7.60 kcal/mole

 

                       3'-GGTTATCATGTCTTTCGTGACTAA-5'

                          xx||||||||||x||||

                 5'-GGCATAAAAATAGTACAGCAAGC-3'

 

GATA51B02-F CATGGATGCAGAATTCACAG versus GATA28D01-R TTTGAAGATCTGTGAAATGTGC

Matches = 11

Score = 9

GCANANTTCACAG

est. tm =  19.1 oC

DeltaG @37 degrees =  -5.29 kcal/mole

 

                    3'-CGTGTAAAGTGTCTAGAAGTTT-5'

                       |||x|x|||||||

             5'-CATGGATGCAGAATTCACAG-3'

 

GATA51B02-F CATGGATGCAGAATTCACAG versus GATA6B09-F TTGCATCCATACGGAAAGTC

Matches = 9

Score = 7

NTGCATCCATN

est. tm =  30.7 oC

DeltaG @37 degrees =  -8.00 kcal/mole

 

                    5'-TTGCATCCATACGGAAAGTC-3'

                       x|||||||||x

           3'-GACACTTAAGACGTAGGTAC-5'

 

GATA51B02-F CATGGATGCAGAATTCACAG versus GATA64H04-R TCTTCATACATGCTTTATCATGC

Matches = 10

Score = 8

TCTNCATNCATG

est. tm =  1.5 oC

DeltaG @37 degrees =  -2.55 kcal/mole

 

                    5'-TCTTCATACATGCTTTATCATGC-3'

                       |||x|||x||||

            3'-GACACTTAAGACGTAGGTAC-5'

 

AGAT113Z-F GGCTCCTGAAAGATTAAAGAAGG versus ATA77F05-F GTTTTTACCTTCATGAATCTCTCC

Matches = 13

Score = 9

NGANAGATTNANGAAGG

est. tm =  14.4 oC

DeltaG @37 degrees =  -3.90 kcal/mole

 

                       3'-CCTCTCTAAGTACTTCCATTTTTG-5'

                          x||x|||||x|x|||||

                 5'-GGCTCCTGAAAGATTAAAGAAGG-3'

 

ATA77F05-F GTTTTTACCTTCATGAATCTCTCC versus GATA28D11-R TAAAAAGAATGAAGGTAAAAATGTG

Matches = 15

Score = 8

NNANANNNATGAAGGTAAAAAN

est. tm =  23.9 oC

DeltaG @37 degrees =  -5.29 kcal/mole

 

                        5'-TAAAAAGAATGAAGGTAAAAATGTG-3'

                           xx|x|xxx|||||||||||||x

                      3'-CCTCTCTAAGTACTTCCATTTTTG-5'

 

ATA29G03Z-F TTTAACTTACTGGCGCAGAT versus GATA193A07-F AGTCAGTCAAAGGCCTGATG

Matches = 9

Score = 7

AGTNAGTNAAA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.56 kcal/mole

 

                    5'-AGTCAGTCAAAGGCCTGATG-3'

                       |||x|||x|||

           3'-TAGACGCGGTCATTCAATTT-5'

 

GGAA30H04-F ATTCTTCTCCCAAGAATGCC versus GGAA30H04-F ATTCTTCTCCCAAGAATGCC

Matches = 12

Score = 7

ATTCTTNNNNNAAGAAT

est. tm =  8.6 oC

DeltaG @37 degrees =  -2.17 kcal/mole

 

                    5'-ATTCTTCTCCCAAGAATGCC-3'

                       ||||||xxxxx||||||

                 3'-CCGTAAGAACCCTCTTCTTA-5'

 

GGAA30H04-F ATTCTTCTCCCAAGAATGCC versus ATA55A11N-F AGAAGAATCACTTGGACCCA

Matches = 8

Score = 8

AGAAGAAT

est. tm =  6.1 oC

DeltaG @37 degrees =  -3.80 kcal/mole

 

                    5'-AGAAGAATCACTTGGACCCA-3'

                       ||||||||

        3'-CCGTAAGAACCCTCTTCTTA-5'

 

GGAA30H04-F ATTCTTCTCCCAAGAATGCC versus AAAAC001-R CCTTTGGAGAAAACTGAACTCG

Matches = 11

Score = 7

NCTTNGGAGAANANT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.21 kcal/mole

 

                    5'-CCTTTGGAGAAAACTGAACTCG-3'

                       x|||x||||||x|x|

               3'-CCGTAAGAACCCTCTTCTTA-5'

 

GGAA30H04-F ATTCTTCTCCCAAGAATGCC versus GACAG001-R GGGGGAAGTATTTGCAGTCA

Matches = 9

Score = 7

GGGNGAAGNAT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.25 kcal/mole

 

                    5'-GGGGGAAGTATTTGCAGTCA-3'

                       |||x||||x||

           3'-CCGTAAGAACCCTCTTCTTA-5'

 

GGAA30H04-F ATTCTTCTCCCAAGAATGCC versus ATA19H08-R GCATTGTGGGATGAGGTATG

Matches = 13

Score = 7

GCATTNTNGGNNGANGNAT

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                    5'-GCATTGTGGGATGAGGTATG-3'

                       |||||x|x||xx||x|x||

                   3'-CCGTAAGAACCCTCTTCTTA-5'

 

GATA193A07-F AGTCAGTCAAAGGCCTGATG versus GATA64G07-R AGACTTGGACTGAATTACACTGC

Matches = 11

Score = 7

NGNCTTNGACTGANT

est. tm =  0.4 oC

DeltaG @37 degrees =  -2.28 kcal/mole

 

                    5'-AGACTTGGACTGAATTACACTGC-3'

                       x|x|||x||||||x|

               3'-GTAGTCCGGAAACTGACTGA-5'

 

GATA193A07-F AGTCAGTCAAAGGCCTGATG versus GATA87E02N-R TTGATTGACTGCACTTTTCA

Matches = 9

Score = 8

TTGANTGACT

est. tm =  8.4 oC

DeltaG @37 degrees =  -4.03 kcal/mole

 

                    5'-TTGATTGACTGCACTTTTCA-3'

                       ||||x|||||

          3'-GTAGTCCGGAAACTGACTGA-5'

 

GATA193A07-F AGTCAGTCAAAGGCCTGATG versus ATGG002-R CCTACCTTCTCCAGGCCTTT

Matches = 10

Score = 8

AAAGGCCTGNNG

est. tm =  20.2 oC

DeltaG @37 degrees =  -5.78 kcal/mole

 

                    3'-TTTCCGGACCTCTTCCATCC-5'

                       |||||||||xx|

            5'-AGTCAGTCAAAGGCCTGATG-3'

 

GATA193A07-F AGTCAGTCAAAGGCCTGATG versus AATA036-R ACTGACTGCAGCCTTGACCT

Matches = 7

Score = 7

ACTGACT

est. tm =  4.9 oC

DeltaG @37 degrees =  -4.23 kcal/mole

 

                    5'-ACTGACTGCAGCCTTGACCT-3'

                       |||||||

       3'-GTAGTCCGGAAACTGACTGA-5'

 

GATA168F06-F ACAATTCCAGAAACTTCCCC versus GATA73D10N-R AGTAAATGGAATTGCTTGTTACC

Matches = 11

Score = 7

AGTNNNTGGAATTGN

est. tm =  19.5 oC

DeltaG @37 degrees =  -5.25 kcal/mole

 

                    5'-AGTAAATGGAATTGCTTGTTACC-3'

                       |||xxx||||||||x

               3'-CCCCTTCAAAGACCTTAACA-5'

 

GATA168F06-F ACAATTCCAGAAACTTCCCC versus TCTA015-F GAATTGTGTGAGCCGGTTTC

Matches = 7

Score = 7

GAATTGT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -3.16 kcal/mole

 

                    5'-GAATTGTGTGAGCCGGTTTC-3'

                       |||||||

       3'-CCCCTTCAAAGACCTTAACA-5'

 

GATA168F06-F ACAATTCCAGAAACTTCCCC versus GACAG001-R GGGGGAAGTATTTGCAGTCA

Matches = 13

Score = 7

NNANTNCANANACTTCCCC

est. tm =  9.5 oC

DeltaG @37 degrees =  -3.30 kcal/mole

 

                    3'-ACTGACGTTTATGAAGGGGG-5'

                       xx|x|x||x|x||||||||

                   5'-ACAATTCCAGAAACTTCCCC-3'

 

GATA168F06-F ACAATTCCAGAAACTTCCCC versus GATA71C09-R TTGAATTGTTCTGGTACCTCTG

Matches = 8

Score = 7

TNGAATTGT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.14 kcal/mole

 

                    5'-TTGAATTGTTCTGGTACCTCTG-3'

                       |x|||||||

         3'-CCCCTTCAAAGACCTTAACA-5'

 

ATA43A10Z-F AAGCACAGAACTGTTTGGG versus ATT198z-F CGTTTCTGTGCCTTAGTTTGC

Matches = 10

Score = 7

CNNTTCTGTGCNT

est. tm =  6.7 oC

DeltaG @37 degrees =  -3.37 kcal/mole

 

                   5'-CGTTTCTGTGCCTTAGTTTGC-3'

                      |xx||||||||x|

             3'-GGGTTTGTCAAGACACGAA-5'

 

ATT198z-F CGTTTCTGTGCCTTAGTTTGC versus ATA47D07-R TCAGATGACAGACAAACTCAGG

Matches = 9

Score = 7

CCTNAGTTTGN

est. tm =  13.9 oC

DeltaG @37 degrees =  -4.88 kcal/mole

 

                     3'-GGACTCAAACAGACAGTAGACT-5'

                        |||x||||||x

           5'-CGTTTCTGTGCCTTAGTTTGC-3'

 

ATT198z-F CGTTTCTGTGCCTTAGTTTGC versus ATA6C09-R AAAACTCAAGCTCAGAAAGGC

Matches = 15

Score = 10

NAAACTNANGCNCAGAAANG

est. tm =  19.2 oC

DeltaG @37 degrees =  -4.50 kcal/mole

 

                     5'-AAAACTCAAGCTCAGAAAGGC-3'

                        x|||||x|x||x||||||x|

                    3'-CGTTTGATTCCGTGTCTTTGC-5'

 

ATT198z-F CGTTTCTGTGCCTTAGTTTGC versus ATA29C03-R GCACAGAAACAGGAAGTATGC

Matches = 10

Score = 9

GCACAGAAACN

est. tm =  34.0 oC

DeltaG @37 degrees =  -8.62 kcal/mole

 

                     5'-GCACAGAAACAGGAAGTATGC-3'

                        ||||||||||x

           3'-CGTTTGATTCCGTGTCTTTGC-5'

 

ATT198z-F CGTTTCTGTGCCTTAGTTTGC versus GATA42A03-R TGTTATAGCAGCAATAGAAAACTAA

Matches = 8

Score = 7

TTAGTTTNC

est. tm =  less than zero

DeltaG @37 degrees =  -1.45 kcal/mole

 

                     3'-AATCAAAAGATAACGACGATATTGT-5'

                        |||||||x|

         5'-CGTTTCTGTGCCTTAGTTTGC-3'

 

GATA21C12-F AAACAATCAAAGGCCCAGG versus ATT198z-F CGTTTCTGTGCCTTAGTTTGC

Matches = 12

Score = 7

NCAANCNAAGGCNCAGN

est. tm =  18.2 oC

DeltaG @37 degrees =  -5.20 kcal/mole

 

                   3'-CGTTTGATTCCGTGTCTTTGC-5'

                      x|||x|x|||||x|||x

                 5'-AAACAATCAAAGGCCCAGG-3'

 

ATA6C09-R AAAACTCAAGCTCAGAAAGGC versus GATA88H02N-F TGAGTTTTTCCTATTGAGAGTCC

Matches = 8

Score = 8

TGAGTTTT

est. tm =  9.2 oC

DeltaG @37 degrees =  -4.25 kcal/mole

 

                     5'-TGAGTTTTTCCTATTGAGAGTCC-3'

                        ||||||||

        3'-CGGAAAGACTCGAACTCAAAA-5'

 

GATA47F05-R AACAGCAAAAAGACACACAGC versus GATA88H02N-F TGAGTTTTTCCTATTGAGAGTCC

Matches = 11

Score = 7

TGNNTTTTTNCTNTT

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                     5'-TGAGTTTTTCCTATTGAGAGTCC-3'

                        ||xx|||||x||x||

               3'-CGACACACAGAAAAACGACAA-5'

 

GATA50G06-F ATACCTGGAGTCCTTGGTCC versus GGAA8G07-R CCCTGGACTCATGGTACTTG

Matches = 12

Score = 7

CCNNGGACTCNNGGTAN

est. tm =  21.1 oC

DeltaG @37 degrees =  -5.55 kcal/mole

 

                    5'-CCCTGGACTCATGGTACTTG-3'

                       ||xx||||||xx||||x

                 3'-CCTGGTTCCTGAGGTCCATA-5'

 

GATA85D02-F TGTGCATCCTCTATGTCCCT versus GGAT3H10-F GGATGGATGGACATAATGGA

Matches = 11

Score = 8

TCCNNTATGTCCNT

est. tm =  8.3 oC

DeltaG @37 degrees =  -3.47 kcal/mole

 

                    3'-AGGTAATACAGGTAGGTAGG-5'

                       |||xx|||||||x|

              5'-TGTGCATCCTCTATGTCCCT-3'

 

ATA24A08-F GCAGCACTTGGCAAATACTC versus sRA-R CCTTGTCAGCGTTTATTTGCC

Matches = 9

Score = 7

GGCAAATANNC

est. tm =  5.9 oC

DeltaG @37 degrees =  -3.18 kcal/mole

 

                    3'-CCGTTTATTTGCGACTGTTCC-5'

                       ||||||||xx|

           5'-GCAGCACTTGGCAAATACTC-3'

 

GATA197B10-F AGGAAAGAAACAAGTGGGCT versus GATA66B04-F ATGCCCTCTTCTGTCTCTCC

Matches = 14

Score = 9

GGANAGANANAAGNGGGCN

est. tm =  23.2 oC

DeltaG @37 degrees =  -6.02 kcal/mole

 

                    3'-CCTCTCTGTCTTCTCCCGTA-5'

                       |||x|||x|x|||x||||x

                   5'-AGGAAAGAAACAAGTGGGCT-3'

 

GATA197B10-F AGGAAAGAAACAAGTGGGCT versus GGAA9D03-R TTCTTTCCTTACTAAGTTGAGAACG

Matches = 9

Score = 9

TTCTTTCCT

est. tm =  17.9 oC

DeltaG @37 degrees =  -5.49 kcal/mole

 

                    5'-TTCTTTCCTTACTAAGTTGAGAACG-3'

                       |||||||||

         3'-TCGGGTGAACAAAGAAAGGA-5'

 

GATA197B10-F AGGAAAGAAACAAGTGGGCT versus GGAA9D03-R TTCTTTCCTTACTAAGTTGAGAACG

Matches = 10

Score = 7

TTNTTTCNTTNCT

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                    5'-TTCTTTCCTTACTAAGTTGAGAACG-3'

                       ||x||||x||x||

             3'-TCGGGTGAACAAAGAAAGGA-5'

 

GATA197B10-F AGGAAAGAAACAAGTGGGCT versus GATA29B01-F GCGAAGGCCATTAATTTCTT

Matches = 12

Score = 8

AAGAAANNANTGGNCT

est. tm =  2.2 oC

DeltaG @37 degrees =  -2.06 kcal/mole

 

                    3'-TTCTTTAATTACCGGAAGCG-5'

                       ||||||xx|x|||x||

                5'-AGGAAAGAAACAAGTGGGCT-3'

 

GATA197B10-F AGGAAAGAAACAAGTGGGCT versus GGAA29H03N-R AGTTGACTCTTTCCCCAACTA

Matches = 11

Score = 7

ANTTGNNTCTTTCCN

est. tm =  11.0 oC

DeltaG @37 degrees =  -3.80 kcal/mole

 

                    5'-AGTTGACTCTTTCCCCAACTA-3'

                       |x|||xx|||||||x

               3'-TCGGGTGAACAAAGAAAGGA-5'

 

ATA3A07-F GTCTGTAACCTAGGCAGGCA versus GATA44F10-F TTCCAGCCTAGGTAGCAGTG

Matches = 14

Score = 8

TNCCNGCCTAGGTNNCAGNN

est. tm =  25.6 oC

DeltaG @37 degrees =  -6.43 kcal/mole

 

                    5'-TTCCAGCCTAGGTAGCAGTG-3'

                       |x||x||||||||xx|||xx

                    3'-ACGGACGGATCCAATGTCTG-5'

 

ATA3A07-F GTCTGTAACCTAGGCAGGCA versus GATA44F10-F TTCCAGCCTAGGTAGCAGTG

Matches = 14

Score = 8

NNCTGNNACCTAGGCNGGNA

est. tm =  24.0 oC

DeltaG @37 degrees =  -6.03 kcal/mole

 

                    3'-GTGACGATGGATCCGACCTT-5'

                       xx|||xx||||||||x||x|

                    5'-GTCTGTAACCTAGGCAGGCA-3'

 

ATA3A07-F GTCTGTAACCTAGGCAGGCA versus GTTTT001-R AGCCTGGGTGACAGAGTGAA

Matches = 12

Score = 8

NGCCTNGGTNACAGAN

est. tm =  33.1 oC

DeltaG @37 degrees =  -8.44 kcal/mole

 

                    5'-AGCCTGGGTGACAGAGTGAA-3'

                       x||||x|||x|||||x

                3'-ACGGACGGATCCAATGTCTG-5'

 

ATA3A07-F GTCTGTAACCTAGGCAGGCA versus GATA21F03-R CCTGCCTGCCTATCTATCTG

Matches = 13

Score = 8

CNGNNANNTAGGCAGGCA

est. tm =  19.6 oC

DeltaG @37 degrees =  -5.15 kcal/mole

 

                    3'-GTCTATCTATCCGTCCGTCC-5'

                       |x|xx|xx||||||||||

                  5'-GTCTGTAACCTAGGCAGGCA-3'

 

ATT001-F GCCATGCAGGAAGGAGTAGA versus GATA66D01-R TAATTCAAATCTTTCCTGTCAGG

Matches = 13

Score = 7

CCNNNCAGGAANGANTNGA

est. tm =  1.5 oC

DeltaG @37 degrees =  -1.62 kcal/mole

 

                    3'-GGACTGTCCTTTCTAAACTTAAT-5'

                       ||xxx||||||x||x|x||

                   5'-GCCATGCAGGAAGGAGTAGA-3'

 

ATT001-F GCCATGCAGGAAGGAGTAGA versus GATA137H02N-R TCTGAGGATTCCTCCTTCCT

Matches = 10

Score = 7

AGGAAGGAGNANN

est. tm =  19.2 oC

DeltaG @37 degrees =  -5.67 kcal/mole

 

                    3'-TCCTTCCTCCTTAGGAGTCT-5'

                       |||||||||x|xx

             5'-GCCATGCAGGAAGGAGTAGA-3'

 

GGAA3G05-F CAGGCTGAATGACAGAACAA versus GATA117D01N-R GATTGATCTGTCTGTCCGTC

Matches = 13

Score = 8

GNCNGANNGACAGANCAA

est. tm =  1.9 oC

DeltaG @37 degrees =  -1.56 kcal/mole

 

                    3'-CTGCCTGTCTGTCTAGTTAG-5'

                       |x|x||xx||||||x|||

                  5'-CAGGCTGAATGACAGAACAA-3'

 

GATA22F09N-F GTTCAAGCATGGGTGGATAG versus GATA73H09N-F CCAGTCTAGACACCCAGGAT

Matches = 11

Score = 7

ANCNTGGGTGNNTAG

est. tm =  less than zero

DeltaG @37 degrees =  -2.09 kcal/mole

 

                    3'-TAGGACCCACAGATCTGACC-5'

                       |x|x||||||xx|||

               5'-GTTCAAGCATGGGTGGATAG-3'

 

GATA22F09N-F GTTCAAGCATGGGTGGATAG versus GGAT3H10-R CACCTTTTCATCTGTCCACC

Matches = 8

Score = 7

GGTGGANAG

est. tm =  7.5 oC

DeltaG @37 degrees =  -4.30 kcal/mole

 

                    3'-CCACCTGTCTACTTTTCCAC-5'

                       ||||||x||

         5'-GTTCAAGCATGGGTGGATAG-3'

 

GATA22F09N-F GTTCAAGCATGGGTGGATAG versus GATA14E09-R GTCATAATATCTGCCAATGATTG

Matches = 12

Score = 7

CAANCATNGGNNGATAN

est. tm =  3.8 oC

DeltaG @37 degrees =  -2.06 kcal/mole

 

                    3'-GTTAGTAACCGTCTATAATACTG-5'

                       |||x|||x||xx||||x

                 5'-GTTCAAGCATGGGTGGATAG-3'

 

GATA22F09N-F GTTCAAGCATGGGTGGATAG versus ATA21A03-R TGCATCCACCTTTGCTATAA

Matches = 13

Score = 7

TTNNAGCANNGGTGGATNN

est. tm =  25.5 oC

DeltaG @37 degrees =  -6.38 kcal/mole

 

                    3'-AATATCGTTTCCACCTACGT-5'

                       ||xx||||xx|||||||xx

                   5'-GTTCAAGCATGGGTGGATAG-3'

 

GATA22F09N-F GTTCAAGCATGGGTGGATAG versus GATA64H04-R TCTTCATACATGCTTTATCATGC

Matches = 13

Score = 7

TNTNCANNCATGCTTNANC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.04 kcal/mole

 

                    5'-TCTTCATACATGCTTTATCATGC-3'

                       |x|x||xx|||||||x|x|

                   3'-GATAGGTGGGTACGAACTTG-5'

 

GATA22F09N-F GTTCAAGCATGGGTGGATAG versus AGAT120-R TTGTCTGTCATCTATCTACCCATC

Matches = 11

Score = 9

NATGGGTNGATAG

est. tm =  23.0 oC

DeltaG @37 degrees =  -6.08 kcal/mole

 

                    3'-CTACCCATCTATCTACTGTCTGTT-5'

                       x||||||x|||||

             5'-GTTCAAGCATGGGTGGATAG-3'

 

GATA22F09N-F GTTCAAGCATGGGTGGATAG versus GGAT3F08-R TATCCATCCATCCATCAACC

Matches = 15

Score = 11

TATCCANCCATNCNTNAAC

est. tm =  15.4 oC

DeltaG @37 degrees =  -3.75 kcal/mole

 

                    5'-TATCCATCCATCCATCAACC-3'

                       ||||||x||||x|x|x|||

                   3'-GATAGGTGGGTACGAACTTG-5'

 

ATACC001-F ATGTTTCATCCCGAAGGACA versus GATA52A04-F TTTGGGATAAAAGGTATTTTGC

Matches = 11

Score = 7

TTNGGGATNAAANNT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.01 kcal/mole

 

                    5'-TTTGGGATAAAAGGTATTTTGC-3'

                       ||x|||||x|||xx|

               3'-ACAGGAAGCCCTACTTTGTA-5'

 

ATACC001-F ATGTTTCATCCCGAAGGACA versus GATA52A04-R TTTGGGATAAAAGGTATTTTGC

Matches = 11

Score = 7

TTNGGGATNAAANNT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.01 kcal/mole

 

                    5'-TTTGGGATAAAAGGTATTTTGC-3'

                       ||x|||||x|||xx|

               3'-ACAGGAAGCCCTACTTTGTA-5'

 

GATA158H04-F ACATACAGCACAGGCCAAAT versus ATCC001-F TGCCCTGTATTCTGTGTAGCA

Matches = 12

Score = 7

TGNCCTGTNNTNTNTGT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.56 kcal/mole

 

                    5'-TGCCCTGTATTCTGTGTAGCA-3'

                       ||x|||||xx|x|x|||

                 3'-TAAACCGGACACGACATACA-5'

 

ATA78D02N-F GGCAAGAGTTTTCCCATTTT versus GATA164A09-R CTCAGTTCAAGGAAATGGGA

Matches = 8

Score = 7

TCCCATTTN

est. tm =  17.4 oC

DeltaG @37 degrees =  -5.77 kcal/mole

 

                    3'-AGGGTAAAGGAACTTGACTC-5'

                       ||||||||x

         5'-GGCAAGAGTTTTCCCATTTT-3'

 

GGAA9D03-F TGGCATGCAATTGTAGTCTC versus GATA6E05-F ATTGCATGGCAACTCTTCTC

Matches = 10

Score = 9

ATTGCATGNCA

est. tm =  17.5 oC

DeltaG @37 degrees =  -5.28 kcal/mole

 

                    5'-ATTGCATGGCAACTCTTCTC-3'

                       ||||||||x||

           3'-CTCTGATGTTAACGTACGGT-5'

 

GGAA9D03-F TGGCATGCAATTGTAGTCTC versus GATA131D09-R AGACTGCAGTGAGATGCCAT

Matches = 14

Score = 9

AGACTNCANTNNNATGCCA

est. tm =  21.1 oC

DeltaG @37 degrees =  -5.25 kcal/mole

 

                    5'-AGACTGCAGTGAGATGCCAT-3'

                       |||||x||x|xxx||||||

                   3'-CTCTGATGTTAACGTACGGT-5'

 

AAT245-F GCCAAGGCAGGAGGATTACT versus TTTA062-F AGCCTCCTACCTTGCCTTCT

Matches = 13

Score = 10

ANCCTCCTNCCTTGNC

est. tm =  20.1 oC

DeltaG @37 degrees =  -5.44 kcal/mole

 

                    5'-AGCCTCCTACCTTGCCTTCT-3'

                       |x||||||x|||||x|

                3'-TCATTAGGAGGACGGAACCG-5'

 

AAT245-F GCCAAGGCAGGAGGATTACT versus GATA137H02N-R TCTGAGGATTCCTCCTTCCT

Matches = 13

Score = 10

AGGNAGGAGGANTNCT

est. tm =  19.8 oC

DeltaG @37 degrees =  -5.50 kcal/mole

 

                    3'-TCCTTCCTCCTTAGGAGTCT-5'

                       |||x|||||||x|x||

                5'-GCCAAGGCAGGAGGATTACT-3'

 

ATA43A10Z-F AAGCACAGAACTGTTTGGG versus AAT269-R CCCAGTGCCCATACACTTTT

Matches = 10

Score = 7

ANAANTGTNTGGG

est. tm =  less than zero

DeltaG @37 degrees =  -2.19 kcal/mole

 

                   3'-TTTTCACATACCCGTGACCC-5'

                      |x||x|||x||||

             5'-AAGCACAGAACTGTTTGGG-3'

 

ATA43A10Z-F AAGCACAGAACTGTTTGGG versus GGAT1A4-R ATAGTTTTGTGCTTATTGACATGA

Matches = 12

Score = 10

ANAGTTNTGTGCTT

est. tm =  22.1 oC

DeltaG @37 degrees =  -5.86 kcal/mole

 

                   5'-ATAGTTTTGTGCTTATTGACATGA-3'

                      |x||||x|||||||

              3'-GGGTTTGTCAAGACACGAA-5'

 

ATA43A10Z-F AAGCACAGAACTGTTTGGG versus GATA48E02-R CAGATTTCTGCTTTCCCAGA

Matches = 10

Score = 7

CAGNTNTNTGCTT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.89 kcal/mole

 

                   5'-CAGATTTCTGCTTTCCCAGA-3'

                      |||x|x|x|||||

             3'-GGGTTTGTCAAGACACGAA-5'

 

TTCA006-F TCCCCACGTAAGCATTTCTC versus GATA6C01-F TCTAGGATGTTTTTAGAGAAAGGC

Matches = 8

Score = 7

GCNTTTCTC

est. tm =  3.0 oC

DeltaG @37 degrees =  -3.31 kcal/mole

 

                    3'-CGGAAAGAGATTTTTGTAGGATCT-5'

                       ||x||||||

         5'-TCCCCACGTAAGCATTTCTC-3'

 

TTCA006-F TCCCCACGTAAGCATTTCTC versus GATA49D12N-R AGATAGAGGGAAAGGCATATGTG

Matches = 12

Score = 8

CACNTANGCNTTTCNC

est. tm =  less than zero

DeltaG @37 degrees =  -1.04 kcal/mole

 

                    3'-GTGTATACGGAAAGGGAGATAGA-5'

                       |||x||x||x||||x|

                5'-TCCCCACGTAAGCATTTCTC-3'

 

ATA5A04N-R TTCAGTCTTGATCTAGTGCTCC versus GATA178F11Z-F TTAAGAATGAAATGATCTAATTCCG

Matches = 9

Score = 7

TNAAGANTGAA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.45 kcal/mole

 

                      5'-TTAAGAATGAAATGATCTAATTCCG-3'

                         |x||||x||||

           3'-CCTCGTGATCTAGTTCTGACTT-5'

 

GGAA2A03-F CCTGGGCAACAGAATAAGAT versus GATA178F11Z-F TTAAGAATGAAATGATCTAATTCCG

Matches = 9

Score = 7

CNGAATNAGAT

est. tm =  less than zero

DeltaG @37 degrees =  -0.79 kcal/mole

 

                    3'-GCCTTAATCTAGTAAAGTAAGAATT-5'

                       |x||||x||||

           5'-CCTGGGCAACAGAATAAGAT-3'

 

GATA189C06-R GGAAGCCAAATGTTGAATTG versus AGAT060-F CATGTAAGATTTTGCTGCCAGA

Matches = 13

Score = 7

NATNNAANATTTNGCTNCC

est. tm =  1.4 oC

DeltaG @37 degrees =  -1.42 kcal/mole

 

                    5'-CATGTAAGATTTTGCTGCCAGA-3'

                       x||xx||x||||x|||x||

                   3'-GTTAAGTTGTAAACCGAAGG-5'

 

GATA11A06-F TTTCCAGTGGAAACCAAACT versus GATA11A06-F TTTCCAGTGGAAACCAAACT

Matches = 12

Score = 11

TTTCCANTGGAAA

est. tm =  28.2 oC

DeltaG @37 degrees =  -7.15 kcal/mole

 

                    5'-TTTCCAGTGGAAACCAAACT-3'

                       ||||||x||||||

             3'-TCAAACCAAAGGTGACCTTT-5'

 

GATA11A06-F TTTCCAGTGGAAACCAAACT versus GATA2A12-F TGTGTTTGGGTTTCCTCTGT

Matches = 12

Score = 7

NCAGNGGAAACCNAANN

est. tm =  22.3 oC

DeltaG @37 degrees =  -5.83 kcal/mole

 

                    3'-TGTCTCCTTTGGGTTTGTGT-5'

                       x|||x|||||||x||xx

                 5'-TTTCCAGTGGAAACCAAACT-3'

 

GATA11A06-F TTTCCAGTGGAAACCAAACT versus GATA4H03-R TCCAATGGAAAGAAATGGAG

Matches = 10

Score = 9

TCCANTGGAAA

est. tm =  19.1 oC

DeltaG @37 degrees =  -5.68 kcal/mole

 

                    5'-TCCAATGGAAAGAAATGGAG-3'

                       ||||x||||||

           3'-TCAAACCAAAGGTGACCTTT-5'

 

GATA11A06-F TTTCCAGTGGAAACCAAACT versus GATA2H09-F TTGCCACTGAAAATCATATCC

Matches = 11

Score = 9

TTNCCACTGNAAA

est. tm =  11.4 oC

DeltaG @37 degrees =  -4.02 kcal/mole

 

                    5'-TTGCCACTGAAAATCATATCC-3'

                       ||x||||||x|||

             3'-TCAAACCAAAGGTGACCTTT-5'

 

GATA11A06-F TTTCCAGTGGAAACCAAACT versus GATA21C12-R CCCATTGGAAACAGAGTTGA

Matches = 9

Score = 7

NCCANTGGAAA

est. tm =  13.7 oC

DeltaG @37 degrees =  -5.00 kcal/mole

 

                    5'-CCCATTGGAAACAGAGTTGA-3'

                       x|||x||||||

           3'-TCAAACCAAAGGTGACCTTT-5'

 

GATA13-F TCATGTGACAAAAGCCACAC versus GATA29A05-F TTTTGTTTCTTGATCTGGGC

Matches = 10

Score = 7

TTTTGTNNCNTGA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.82 kcal/mole

 

                    5'-TTTTGTTTCTTGATCTGGGC-3'

                       ||||||xx|x|||

             3'-CACACCGAAAACAGTGTACT-5'

 

GATA13-F TCATGTGACAAAAGCCACAC versus GATA165G02N-R CTGTCACAGGACTTTTCAGCC

Matches = 9

Score = 7

NTGTCACANGA

est. tm =  14.4 oC

DeltaG @37 degrees =  -4.99 kcal/mole

 

                    5'-CTGTCACAGGACTTTTCAGCC-3'

                       x|||||||x||

           3'-CACACCGAAAACAGTGTACT-5'

 

ATA23G05-F AGCTGGAGAGGGATAGCATT versus GATA23D03N-R ATTCTTTGTCTCCCCAGTTG

Matches = 13

Score = 7

ATNCTNTNNCTCNCCAGNT

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                    5'-ATTCTTTGTCTCCCCAGTTG-3'

                       ||x||x|xx|||x||||x|

                   3'-TTACGATAGGGAGAGGTCGA-5'

 

GATA7E12-F TCAAATTTTTAAGTCTCACCAGG versus GATA131D09-F AAAAATCTGACTCTATTCAAATGTT

Matches = 9

Score = 8

AAAAATNTGA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.44 kcal/mole

 

                       5'-AAAAATCTGACTCTATTCAAATGTT-3'

                          ||||||x|||

          3'-GGACCACTCTGAATTTTTAAACT-5'

 

GATA74E02Z-R TGAGCCTTTAGACTTGGACCA versus GATA7E12-F TCAAATTTTTAAGTCTCACCAGG

Matches = 13

Score = 8

TCNAANTNTNAAGNCTCA

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                     5'-TCAAATTTTTAAGTCTCACCAGG-3'

                        ||x||x|x|x|||x||||

                  3'-ACCAGGTTCAGATTTCCGAGT-5'

 

GATA177C03N-F CTCTCTTCATCCACCATTGG versus UT1355Z-F AGGATGGATAAATAGAACATAGGTAGA

Matches = 12

Score = 7

ANGNTGGATNAANAGAN

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.68 kcal/mole

 

                    5'-AGGATGGATAAATAGAACATAGGTAGA-3'

                       |x|x|||||x||x|||x

                 3'-GGTTACCACCTACTTCTCTC-5'

 

GATA21G05-F AGGCTGTGGTGAGCTATGAC versus GATA72H07-R AGCTCACCTCCTCCAAGAAG

Matches = 11

Score = 7

AGCTCACCNCNNCCN

est. tm =  22.3 oC

DeltaG @37 degrees =  -6.24 kcal/mole

 

                    5'-AGCTCACCTCCTCCAAGAAG-3'

                       ||||||||x|xx||x

               3'-CAGTATCGAGTGGTGTCGGA-5'

 

GATA21G05-F AGGCTGTGGTGAGCTATGAC versus ATA12D05-R CACCTCAGCCTCTTTGGTAG

Matches = 10

Score = 9

CACCNCAGCCT

est. tm =  27.5 oC

DeltaG @37 degrees =  -7.34 kcal/mole

 

                    5'-CACCTCAGCCTCTTTGGTAG-3'

                       ||||x||||||

           3'-CAGTATCGAGTGGTGTCGGA-5'

 

GATA66B04-F ATGCCCTCTTCTGTCTCTCC versus GATA13G11-R GGGTTCTGCAGAGAAACAGA

Matches = 9

Score = 7

TCTGTNTCTCN

est. tm =  13.5 oC

DeltaG @37 degrees =  -4.91 kcal/mole

 

                    3'-AGACAAAGAGACGTCTTGGG-5'

                       |||||x||||x

           5'-ATGCCCTCTTCTGTCTCTCC-3'

 

GATA66B04-F ATGCCCTCTTCTGTCTCTCC versus GATA68F07-R GACAGGAGACAGAATGTTATAAGTT

Matches = 11

Score = 7

GACAGNAGANNGNAT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.17 kcal/mole

 

                    5'-GACAGGAGACAGAATGTTATAAGTT-3'

                       |||||x|||xx|x||

               3'-CCTCTCTGTCTTCTCCCGTA-5'

 

GATA66B04-F ATGCCCTCTTCTGTCTCTCC versus GATA7G10-R GCCCAAAAAGACAGACAGAA

Matches = 10

Score = 8

TTCTGTCTNTCN

est. tm =  19.5 oC

DeltaG @37 degrees =  -5.64 kcal/mole

 

                    3'-AAGACAGACAGAAAAACCCG-5'

                       ||||||||x||x

            5'-ATGCCCTCTTCTGTCTCTCC-3'

 

Mfd235-F ACTTTCTTGCAGTCTGTGGC versus ATTT019-R TTCATGGCCACAGAATTCAC

Matches = 11

Score = 8

NTGNANTCTGTGGC

est. tm =  23.7 oC

DeltaG @37 degrees =  -6.39 kcal/mole

 

                    3'-CACTTAAGACACCGGTACTT-5'

                       x||x|x||||||||

              5'-ACTTTCTTGCAGTCTGTGGC-3'

 

Mfd235-F ACTTTCTTGCAGTCTGTGGC versus GATA32B03-R AGGTATGGTGCCACACACTT

Matches = 9

Score = 7

NAGTNTGTGGC

est. tm =  21.6 oC

DeltaG @37 degrees =  -6.31 kcal/mole

 

                    3'-TTCACACACCGTGGTATGGA-5'

                       x|||x||||||

           5'-ACTTTCTTGCAGTCTGTGGC-3'

 

Mfd232-F AGAGTGAGATTCCACCTTTC versus ATT015-R TCACACTCTGGGGACTGTTG

Matches = 8

Score = 7

TCNCACTCT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -3.13 kcal/mole

 

                    5'-TCACACTCTGGGGACTGTTG-3'

                       ||x||||||

         3'-CTTTCCACCTTAGAGTGAGA-5'

 

GATA145D10N-R TTGAGCCCAGAAGTTTGAG versus Mfd238-F TTCTGAGCACAACCAATCCT

Matches = 10

Score = 8

TTCTGNGCNCAA

est. tm =  6.4 oC

DeltaG @37 degrees =  -3.55 kcal/mole

 

                   5'-TTCTGAGCACAACCAATCCT-3'

                      |||||x||x|||

            3'-GAGTTTGAAGACCCGAGTT-5'

 

Mfd238-F TTCTGAGCACAACCAATCCT versus GATA13G11-R GGGTTCTGCAGAGAAACAGA

Matches = 11

Score = 7

NGGTTNTGCNNAGAA

est. tm =  15.7 oC

DeltaG @37 degrees =  -4.75 kcal/mole

 

                    5'-GGGTTCTGCAGAGAAACAGA-3'

                       x||||x|||xx||||

               3'-TCCTAACCAACACGAGTCTT-5'

 

Mfd238-F TTCTGAGCACAACCAATCCT versus GATA2F11-R AAGCAAAGAGGATTGAAAGTG

Matches = 10

Score = 7

CACNNNCAATCCT

est. tm =  11.2 oC

DeltaG @37 degrees =  -3.83 kcal/mole

 

                    3'-GTGAAAGTTAGGAGAAACGAA-5'

                       |||xxx|||||||

             5'-TTCTGAGCACAACCAATCCT-3'

 

GATA115E01N-R CAGACAGATTCCTGTGGT versus GATA51D03-F AGCCTCCATAACCACATGAA

Matches = 9

Score = 8

TTCNTGTGGT

est. tm =  11.9 oC

DeltaG @37 degrees =  -4.71 kcal/mole

 

                  3'-AAGTACACCAATACCTCCGA-5'

                     |||x||||||

          5'-CAGACAGATTCCTGTGGT-3'

 

GATA51D03-F AGCCTCCATAACCACATGAA versus GATA27A12-F GTGGCTTTGGAGGAACACTA

Matches = 11

Score = 7

GTGGNTNTGGAGGNN

est. tm =  21.8 oC

DeltaG @37 degrees =  -6.07 kcal/mole

 

                    5'-GTGGCTTTGGAGGAACACTA-3'

                       ||||x|x||||||xx

               3'-AAGTACACCAATACCTCCGA-5'

 

GATA51D03-F AGCCTCCATAACCACATGAA versus GGAA5C04Z-F GAGCTTAAGGAGGCCAAGGT

Matches = 11

Score = 7

GNGNTTANGGAGGCN

est. tm =  8.3 oC

DeltaG @37 degrees =  -3.71 kcal/mole

 

                    5'-GAGCTTAAGGAGGCCAAGGT-3'

                       |x|x|||x||||||x

               3'-AAGTACACCAATACCTCCGA-5'

 

GATA64A09-R CAGAGTGAGACCCTGTTTCA versus GATA129B03N-F GTGTTCTCCAGTGAAACAGAGC

Matches = 9

Score = 7

NCNCTGTTTCA

est. tm =  9.4 oC

DeltaG @37 degrees =  -4.08 kcal/mole

 

                    3'-CGAGACAAAGTGACCTCTTGTG-5'

                       x|x||||||||

           5'-CAGAGTGAGACCCTGTTTCA-3'

 

GATA4H01-F AAACCTCTCTTTGATTGGGG versus GATA129B03N-F GTGTTCTCCAGTGAAACAGAGC

Matches = 12

Score = 7

NCTCTNTTTNANTGGNG

est. tm =  less than zero

DeltaG @37 degrees =  -1.45 kcal/mole

 

                    3'-CGAGACAAAGTGACCTCTTGTG-5'

                       x||||x|||x|x|||x|

                 5'-AAACCTCTCTTTGATTGGGG-3'

 

GATA25A04-F TAGCACTTGAGCACACATGG versus GATA129B03N-F GTGTTCTCCAGTGAAACAGAGC

Matches = 12

Score = 8

GTGTNCTCNAGTGNNA

est. tm =  8.4 oC

DeltaG @37 degrees =  -3.07 kcal/mole

 

                    5'-GTGTTCTCCAGTGAAACAGAGC-3'

                       ||||x|||x||||xx|

                3'-GGTACACACGAGTTCACGAT-5'

 

GATA45B10N-F GTGGTGAACACCAATAAATGG versus GATA129B03N-F GTGTTCTCCAGTGAAACAGAGC

Matches = 9

Score = 7

GTGTTCNCCAN

est. tm =  17.5 oC

DeltaG @37 degrees =  -5.52 kcal/mole

 

                     5'-GTGTTCTCCAGTGAAACAGAGC-3'

                        ||||||x|||x

           3'-GGTAAATAACCACAAGTGGTG-5'

 

GATA2A12-F TGTGTTTGGGTTTCCTCTGT versus GATA42A03-F CCAAAGACAAGTTTGTTTATCTG

Matches = 8

Score = 7

CCAAANACA

est. tm =  1.1 oC

DeltaG @37 degrees =  -3.30 kcal/mole

 

                    5'-CCAAAGACAAGTTTGTTTATCTG-3'

                       |||||x|||

         3'-TGTCTCCTTTGGGTTTGTGT-5'

 

GATA13G11-R GGGTTCTGCAGAGAAACAGA versus GATA42A03-F CCAAAGACAAGTTTGTTTATCTG

Matches = 10

Score = 8

CAGANAAACANA

est. tm =  3.5 oC

DeltaG @37 degrees =  -2.83 kcal/mole

 

                    3'-GTCTATTTGTTTGAACAGAAACC-5'

                       ||||x|||||x|

            5'-GGGTTCTGCAGAGAAACAGA-3'

 

GATA4H01-F AAACCTCTCTTTGATTGGGG versus GATA42A03-F CCAAAGACAAGTTTGTTTATCTG

Matches = 11

Score = 8

NCAAAGANANGTTT

est. tm =  9.4 oC

DeltaG @37 degrees =  -3.41 kcal/mole

 

                    5'-CCAAAGACAAGTTTGTTTATCTG-3'

                       x||||||x|x||||

              3'-GGGGTTAGTTTCTCTCCAAA-5'

 

AAT269-F GTCGAATGATGGCTTTCCTG versus GATA11A11-R TCTGGAAAACCCTAATTCTCC

Matches = 14

Score = 8

GNNGAATNANGGNTTTCCNG

est. tm =  less than zero

DeltaG @37 degrees =  -0.24 kcal/mole

 

                    3'-CCTCTTAATCCCAAAAGGTCT-5'

                       |xx||||x|x||x|||||x|

                    5'-GTCGAATGATGGCTTTCCTG-3'

 

AAAT072-R ACTCCACCCAGGAAGTCCA versus AAT269-F GTCGAATGATGGCTTTCCTG

Matches = 9

Score = 7

CAGGAANNCCA

est. tm =  10.3 oC

DeltaG @37 degrees =  -4.30 kcal/mole

 

                   3'-GTCCTTTCGGTAGTAAGCTG-5'

                      ||||||xx|||

           5'-ACTCCACCCAGGAAGTCCA-3'

 

AAT269-F GTCGAATGATGGCTTTCCTG versus Mfd232-R GAAACACATCATTTACCCAC

Matches = 12

Score = 7

GAAANNCATCATTNNNC

est. tm =  5.8 oC

DeltaG @37 degrees =  -1.62 kcal/mole

 

                    5'-GAAACACATCATTTACCCAC-3'

                       ||||xx|||||||xxx|

                 3'-GTCCTTTCGGTAGTAAGCTG-5'

 

AAT269-F GTCGAATGATGGCTTTCCTG versus GATA64D02-F TATTTCAAAGGCAGCAAAGC

Matches = 8

Score = 7

GCTTTNCTG

est. tm =  3.5 oC

DeltaG @37 degrees =  -3.43 kcal/mole

 

                    3'-CGAAACGACGGAAACTTTAT-5'

                       |||||x|||

         5'-GTCGAATGATGGCTTTCCTG-3'

 

GATA158H04-R CCAGTCTTCCGTCACTATGC versus UT254-F TCTCAGTGAAGGTAGACAGGA

Matches = 14

Score = 8

NCNNAGTGANGGNAGACNGG

est. tm =  16.0 oC

DeltaG @37 degrees =  -4.23 kcal/mole

 

                    5'-TCTCAGTGAAGGTAGACAGGA-3'

                       x|xx|||||x||x||||x||

                    3'-CGTATCACTGCCTTCTGACC-5'

 

UT254-F TCTCAGTGAAGGTAGACAGGA versus GATA121A08N-F GTTAACAGACTATTACCTGCCTACC

Matches = 9

Score = 7

GGTAGNCAGGN

est. tm =  15.6 oC

DeltaG @37 degrees =  -5.27 kcal/mole

 

                     3'-CCATCCGTCCATTATCAGACAATTG-5'

                        |||||x||||x

           5'-TCTCAGTGAAGGTAGACAGGA-3'

 

UT254-F TCTCAGTGAAGGTAGACAGGA versus MFD427-AAAT028-R GGTTTCCTAACTCTTTTCCCTGA

Matches = 13

Score = 7

TCAGNGAANNNAGNNAGGA

est. tm =  6.8 oC

DeltaG @37 degrees =  -2.30 kcal/mole

 

                     3'-AGTCCCTTTTCTCAATCCTTTGG-5'

                        ||||x|||xxx||xx||||

                   5'-TCTCAGTGAAGGTAGACAGGA-3'

 

GATA64D03-R CCTCACTGAGTCATGTTGCA versus UT254-F TCTCAGTGAAGGTAGACAGGA

Matches = 9

Score = 7

NCTCAGTGANG

est. tm =  13.4 oC

DeltaG @37 degrees =  -4.71 kcal/mole

 

                    5'-TCTCAGTGAAGGTAGACAGGA-3'

                       x||||||||x|

           3'-ACGTTGTACTGAGTCACTCC-5'

 

GGAA3C07-F ATGTACATGTGTCTGGGAAGG versus GGAA3C07-F ATGTACATGTGTCTGGGAAGG

Matches = 8

Score = 8

ATGTACAT

est. tm =  8.7 oC

DeltaG @37 degrees =  -3.42 kcal/mole

 

                     5'-ATGTACATGTGTCTGGGAAGG-3'

                        ||||||||

        3'-GGAAGGGTCTGTGTACATGTA-5'

 

GATA4E11-R CAGACACAAATGCACACACA versus GGAA3C07-F ATGTACATGTGTCTGGGAAGG

Matches = 11

Score = 7

NTGNANNTGTGTCTG

est. tm =  15.7 oC

DeltaG @37 degrees =  -4.56 kcal/mole

 

                    5'-ATGTACATGTGTCTGGGAAGG-3'

                       x||x|xx||||||||

               3'-ACACACACGTAAACACAGAC-5'

 

GGAA3C07-F ATGTACATGTGTCTGGGAAGG versus GATA91H06-F ACACAGATGTGCATCTATCTCG

Matches = 11

Score = 8

ANACANATGTNCAT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.83 kcal/mole

 

                     5'-ACACAGATGTGCATCTATCTCG-3'

                        |x|||x||||x|||

              3'-GGAAGGGTCTGTGTACATGTA-5'

 

GATA27A12-R TGGCACATGTACACCAGAAC versus GGAA3C07-F ATGTACATGTGTCTGGGAAGG

Matches = 11

Score = 8

NTGTACATGTGNCN

est. tm =  26.9 oC

DeltaG @37 degrees =  -6.92 kcal/mole

 

                    5'-ATGTACATGTGTCTGGGAAGG-3'

                       x||||||||||x|x

              3'-CAAGACCACATGTACACGGT-5'

 

GATA48E02-R CAGATTTCTGCTTTCCCAGA versus GGAA3C07-F ATGTACATGTGTCTGGGAAGG

Matches = 9

Score = 8

CNTTCCCAGA

est. tm =  12.9 oC

DeltaG @37 degrees =  -4.84 kcal/mole

 

                    3'-GGAAGGGTCTGTGTACATGTA-5'

                       |x||||||||

          5'-CAGATTTCTGCTTTCCCAGA-3'

 

PAH-R AATCATAAGTGTTCCCAGAC versus GGAA3C07-F ATGTACATGTGTCTGGGAAGG

Matches = 9

Score = 7

NNTTCCCAGAC

est. tm =  24.3 oC

DeltaG @37 degrees =  -6.64 kcal/mole

 

                    3'-GGAAGGGTCTGTGTACATGTA-5'

                       xx|||||||||

           5'-AATCATAAGTGTTCCCAGAC-3'

 

GGAA3C07-F ATGTACATGTGTCTGGGAAGG versus ATA29A06N-R ACCACACACAGCTACTTTTGC

Matches = 12

Score = 7

NCCANACACANNTACNT

est. tm =  9.4 oC

DeltaG @37 degrees =  -3.35 kcal/mole

 

                     5'-ACCACACACAGCTACTTTTGC-3'

                        x|||x|||||xx|||x|

                 3'-GGAAGGGTCTGTGTACATGTA-5'

 

GATA3E10-R GGGGGAAAAAGAACACTGTA versus ATA27F01-F GAGTTTGAAAATAAAGTGTTCTGC

Matches = 10

Score = 7

NNAGAACACTNTA

est. tm =  11.7 oC

DeltaG @37 degrees =  -3.98 kcal/mole

 

                    3'-CGTCTTGTGAAATAAAAGTTTGAG-5'

                       xx||||||||x||

             5'-GGGGGAAAAAGAACACTGTA-3'

 

AATA058-R GGGAGGACATTGCAGAAAAC versus ATA27F01-F GAGTTTGAAAATAAAGTGTTCTGC

Matches = 8

Score = 7

GCAGAANAC

est. tm =  6.4 oC

DeltaG @37 degrees =  -3.89 kcal/mole

 

                    3'-CGTCTTGTGAAATAAAAGTTTGAG-5'

                       ||||||x||

         5'-GGGAGGACATTGCAGAAAAC-3'

 

AGAT120-R TTGTCTGTCATCTATCTACCCATC versus UT1355Z-F AGGATGGATAAATAGAACATAGGTAGA

Matches = 16

Score = 8

GATGGNTANATAGANNANAGNNAN

est. tm =  5.3 oC

DeltaG @37 degrees =  -0.90 kcal/mole

 

                        5'-AGGATGGATAAATAGAACATAGGTAGA-3'

                             |||||x||x|||||xx|x||xx|x

                          3'-CTACCCATCTATCTACTGTCTGTT-5'

 

GGAT3F08-R TATCCATCCATCCATCAACC versus UT1355Z-F AGGATGGATAAATAGAACATAGGTAGA

Matches = 9

Score = 8

NGGATGGATA

est. tm =  19.7 oC

DeltaG @37 degrees =  -5.84 kcal/mole

 

                    5'-AGGATGGATAAATAGAACATAGGTAGA-3'

                       x|||||||||

          3'-CCAACTACCTACCTACCTAT-5'

 

GGAT3F08-R TATCCATCCATCCATCAACC versus UT1355Z-F AGGATGGATAAATAGAACATAGGTAGA

Matches = 11

Score = 8

NGGATGGATNNATA

est. tm =  16.9 oC

DeltaG @37 degrees =  -4.79 kcal/mole

 

                    5'-AGGATGGATAAATAGAACATAGGTAGA-3'

                       x||||||||xx|||

              3'-CCAACTACCTACCTACCTAT-5'

 

GATA198B05N-F GGAGCCAATTCACCTAAT versus GATA23F05-F ATGGTATTAGGAGATGGGGC

Matches = 11

Score = 7

GCCNNNTCNCCTAAT

est. tm =  7.9 oC

DeltaG @37 degrees =  -3.15 kcal/mole

 

                  3'-CGGGGTAGAGGATTATGGTA-5'

                     |||xxx||x||||||

               5'-GGAGCCAATTCACCTAAT-3'

 

ATTT019-F GCTGCCACTTTCCCTTTTAC versus GATA8C04-R TAAAATAGAAAGTGCCCCTCC

Matches = 13

Score = 7

TAAAANNGAAAGTGNCNNN

est. tm =  12.1 oC

DeltaG @37 degrees =  -2.81 kcal/mole

 

                    5'-TAAAATAGAAAGTGCCCCTCC-3'

                       |||||xx|||||||x|xxx

                   3'-CATTTTCCCTTTCACCGTCG-5'

 

GATA2A12-F TGTGTTTGGGTTTCCTCTGT versus GATA11B12-F AACAAAACAAAACAAAACAAACA

Matches = 14

Score = 8

ACANANNAAANCNAAACANA

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                    5'-AACAAAACAAAACAAAACAAACA-3'

                        |||x|xx|||x|x|||||x|

                     3'-TGTCTCCTTTGGGTTTGTGT-5'

 

GATA29A05-F TTTTGTTTCTTGATCTGGGC versus GATA11B12-F AACAAAACAAAACAAAACAAACA

Matches = 7

Score = 7

AACAAAA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.94 kcal/mole

 

                    5'-AACAAAACAAAACAAAACAAACA-3'

                       |||||||

       3'-CGGGTCTAGTTCTTTGTTTT-5'

 

GATA71C09-R TTGAATTGTTCTGGTACCTCTG versus GATA11B12-F AACAAAACAAAACAAAACAAACA

Matches = 11

Score = 7

ANCANAACAANNCAA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.00 kcal/mole

 

                      5'-AACAAAACAAAACAAAACAAACA-3'

                         |x||x|||||xx|||

               3'-GTCTCCATGGTCTTGTTAAGTT-5'

 

UT7136-F AGGAGGCAGAGGTTACAGTA versus GATA5B02-R CAGACATTCTTTATTCTTTACCTCC

Matches = 13

Score = 7

GGAGGNANAGNNTANAGNA

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                    3'-CCTCCATTTCTTATTTCTTACAGAC-5'

                       |||||x|x||xx||x||x|

                   5'-AGGAGGCAGAGGTTACAGTA-3'

 

UT7136-F AGGAGGCAGAGGTTACAGTA versus ATA12D05-R CACCTCAGCCTCTTTGGTAG

Matches = 11

Score = 8

NACCTCNGCCTCNT

est. tm =  22.9 oC

DeltaG @37 degrees =  -6.38 kcal/mole

 

                    5'-CACCTCAGCCTCTTTGGTAG-3'

                       x|||||x|||||x|

              3'-ATGACATTGGAGACGGAGGA-5'

 

UT7136-F AGGAGGCAGAGGTTACAGTA versus GATA71C09-R TTGAATTGTTCTGGTACCTCTG

Matches = 11

Score = 8

CAGAGGTNNCAGNA

est. tm =  13.1 oC

DeltaG @37 degrees =  -4.24 kcal/mole

 

                    3'-GTCTCCATGGTCTTGTTAAGTT-5'

                       |||||||xx|||x|

              5'-AGGAGGCAGAGGTTACAGTA-3'

 

UT7136-F AGGAGGCAGAGGTTACAGTA versus GATA185H04N-R ATATTTCAATATTGTAACCAGTCC

Matches = 11

Score = 7

GNANNGGTTACANTA

est. tm =  less than zero

DeltaG @37 degrees =  -0.58 kcal/mole

 

                    3'-CCTGACCAATGTTATAACTTTATA-5'

                       |x|xx|||||||x||

               5'-AGGAGGCAGAGGTTACAGTA-3'

 

GATA2A12-F TGTGTTTGGGTTTCCTCTGT versus ATA47D07-R TCAGATGACAGACAAACTCAGG

Matches = 14

Score = 8

NCAGANGANANNCAAACNCA

est. tm =  9.8 oC

DeltaG @37 degrees =  -2.72 kcal/mole

 

                    5'-TCAGATGACAGACAAACTCAGG-3'

                       x||||x||x|xx|||||x||

                    3'-TGTCTCCTTTGGGTTTGTGT-5'

 

GATA2A12-F TGTGTTTGGGTTTCCTCTGT versus ATA24F10-R GTTACATAGGAAGCCCGGAT

Matches = 12

Score = 7

NTNNGGGNTTCCTNTGT

est. tm =  12.6 oC

DeltaG @37 degrees =  -4.20 kcal/mole

 

                    3'-TAGGCCCGAAGGATACATTG-5'

                       x|xx|||x|||||x|||

                 5'-TGTGTTTGGGTTTCCTCTGT-3'

 

GATA2A12-F TGTGTTTGGGTTTCCTCTGT versus GATA3F01-R CTGAGGTATCAAAAACTCAGAGG

Matches = 13

Score = 7

CNGAGGNANCNNAAACNCA

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                    5'-CTGAGGTATCAAAAACTCAGAGG-3'

                       |x||||x|x|xx||||x||

                   3'-TGTCTCCTTTGGGTTTGTGT-5'

 

GATA2A12-F TGTGTTTGGGTTTCCTCTGT versus GATA46A12-R AAAGAGTAAACTCAATGAAAGATGC

Matches = 14

Score = 8

ANAGAGNAAACNCAANNANA

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                    5'-AAAGAGTAAACTCAATGAAAGATGC-3'

                       |x||||x||||x|||xx|x|

                    3'-TGTCTCCTTTGGGTTTGTGT-5'

 

GATA52B03-F GAGCAATGATCTCATTTGCA versus AGAT060-R TACCATGCCAAGGACATCAA

Matches = 11

Score = 7

NTGATNTCNTTNGCA

est. tm =  3.7 oC

DeltaG @37 degrees =  -2.82 kcal/mole

 

                    3'-AACTACAGGAACCGTACCAT-5'

                       x||||x||x||x|||

               5'-GAGCAATGATCTCATTTGCA-3'

 

GATA52B03-F GAGCAATGATCTCATTTGCA versus TATC043-F GCCATCCTCCTGCAAATAAG

Matches = 9

Score = 8

CTNATTTGCA

est. tm =  9.9 oC

DeltaG @37 degrees =  -4.13 kcal/mole

 

                    3'-GAATAAACGTCCTCCTACCG-5'

                       ||x|||||||

          5'-GAGCAATGATCTCATTTGCA-3'

 

AAAT072-R ACTCCACCCAGGAAGTCCA versus GATA175D03-F TGGAGTCTCTGGGTGAAGAG

Matches = 14

Score = 9

TGGANTNNCTGGGTGNAGN

est. tm =  28.7 oC

DeltaG @37 degrees =  -7.23 kcal/mole

 

                   5'-TGGAGTCTCTGGGTGAAGAG-3'

                      ||||x|xx|||||||x||x

                   3'-ACCTGAAGGACCCACCTCA-5'

 

GATA175D03-F TGGAGTCTCTGGGTGAAGAG versus TTCA004-R CCCAGAGCCTAAATCTGTGC

Matches = 10

Score = 7

CCCAGAGNCTNNA

est. tm =  5.5 oC

DeltaG @37 degrees =  -3.44 kcal/mole

 

                    5'-CCCAGAGCCTAAATCTGTGC-3'

                       |||||||x||xx|

             3'-GAGAAGTGGGTCTCTGAGGT-5'

 

GATA175D03-F TGGAGTCTCTGGGTGAAGAG versus GATA84C01-F CAGACTCCAGAAAACAGAACC

Matches = 8

Score = 7

NAGACTCCA

est. tm =  18.8 oC

DeltaG @37 degrees =  -5.98 kcal/mole

 

                    5'-CAGACTCCAGAAAACAGAACC-3'

                       x||||||||

         3'-GAGAAGTGGGTCTCTGAGGT-5'

 

GATA175D03-F TGGAGTCTCTGGGTGAAGAG versus MFD427-AAAT028-R GGTTTCCTAACTCTTTTCCCTGA

Matches = 10

Score = 7

TCNGGGNNAAGAG

est. tm =  3.4 oC

DeltaG @37 degrees =  -3.02 kcal/mole

 

                    3'-AGTCCCTTTTCTCAATCCTTTGG-5'

                       ||x|||xx|||||

             5'-TGGAGTCTCTGGGTGAAGAG-3'

 

GATA124E07-F CCAGCCTGGCTGTTAGAGTA versus GATA124E07-F CCAGCCTGGCTGTTAGAGTA

Matches = 8

Score = 7

CCAGNCTGG

est. tm =  7.3 oC

DeltaG @37 degrees =  -4.28 kcal/mole

 

                    5'-CCAGCCTGGCTGTTAGAGTA-3'

                       ||||x||||

         3'-ATGAGATTGTCGGTCCGACC-5'

 

GATA124E07-F CCAGCCTGGCTGTTAGAGTA versus GATA124E07-F CCAGCCTGGCTGTTAGAGTA

Matches = 10

Score = 7

NCAGCCNGGCTGN

est. tm =  36.0 oC

DeltaG @37 degrees =  -9.16 kcal/mole

 

                    5'-CCAGCCTGGCTGTTAGAGTA-3'

                       x|||||x|||||x

             3'-ATGAGATTGTCGGTCCGACC-5'

 

AAT193-F CACAACAGCGAAACTCCATC versus GGAA6D03N-R CTCAGATGGAGATTCCCTCC

Matches = 12

Score = 8

NNAGNGAANCTCCATC

est. tm =  17.3 oC

DeltaG @37 degrees =  -4.62 kcal/mole

 

                    3'-CCTCCCTTAGAGGTAGACTC-5'

                       xx||x|||x|||||||

                5'-CACAACAGCGAAACTCCATC-3'

 

AAT193-F CACAACAGCGAAACTCCATC versus AAT013-F GCTGTGGTGAGCTGTGATTG

Matches = 8

Score = 7

GCTGTNGTG

est. tm =  9.7 oC

DeltaG @37 degrees =  -4.47 kcal/mole

 

                    5'-GCTGTGGTGAGCTGTGATTG-3'

                       |||||x|||

         3'-CTACCTCAAAGCGACAACAC-5'

 

GATA7D12-R ACCTAAATGTTCATCAAAAGAGG versus GATA31F01-F TTGGTACTTAATAAACCCTCTTTT

Matches = 8

Score = 8

AAAAGAGG

est. tm =  11.0 oC

DeltaG @37 degrees =  -4.51 kcal/mole

 

                       3'-TTTTCTCCCAAATAATTCATGGTT-5'

                          ||||||||

        5'-ACCTAAATGTTCATCAAAAGAGG-3'

 

GATA164A09-F AGAGCCCATGGAGCTATCTT versus GATA23F06-R GAGTAAACAAGATTGCTAGATAGGC

Matches = 13

Score = 9

GCCNATNNAGCNATCTT

est. tm =  17.0 oC

DeltaG @37 degrees =  -4.56 kcal/mole

 

                    3'-CGGATAGATCGTTAGAACAAATGAG-5'

                       |||x||xx|||x|||||

                 5'-AGAGCCCATGGAGCTATCTT-3'

 

GATA85D02-R GCTAAGATGGCGCCATTG versus GATA164A09-F AGAGCCCATGGAGCTATCTT

Matches = 12

Score = 9

AAGATNGCNCCATNG

est. tm =  9.6 oC

DeltaG @37 degrees =  -3.46 kcal/mole

 

                  3'-TTCTATCGAGGTACCCGAGA-5'

                     |||||x||x||||x|

               5'-GCTAAGATGGCGCCATTG-3'

 

ATCT003-F GGCCTCCAGCTTACAGACAG versus ATA26D07-R GGCCCTGTGTAGAAGCTGTA

Matches = 12

Score = 8

TNCAGCTTNNANACAG

est. tm =  9.9 oC

DeltaG @37 degrees =  -3.38 kcal/mole

 

                    3'-ATGTCGAAGATGTGTCCCGG-5'

                       |x||||||xx|x||||

                5'-GGCCTCCAGCTTACAGACAG-3'

 

CTAT014-F TTTGTGGGGAGCCCTAGAC versus ATA5A04N-R TTCAGTCTTGATCTAGTGCTCC

Matches = 10

Score = 8

GGAGCNCTAGAN

est. tm =  23.3 oC

DeltaG @37 degrees =  -6.40 kcal/mole

 

                   3'-CCTCGTGATCTAGTTCTGACTT-5'

                      |||||x|||||x

            5'-TTTGTGGGGAGCCCTAGAC-3'

 

CTAT014-F TTTGTGGGGAGCCCTAGAC versus GATA187D09N-R CCACAAATACATTTCCCTGA

Matches = 7

Score = 7

CCACAAA

est. tm =  6.5 oC

DeltaG @37 degrees =  -4.26 kcal/mole

 

                   5'-CCACAAATACATTTCCCTGA-3'

                      |||||||

       3'-CAGATCCCGAGGGGTGTTT-5'

 

TTTA062-F AGCCTCCTACCTTGCCTTCT versus AAT193-R GCAAGGATGCAGAAGGAAAG

Matches = 9

Score = 8

CTTNCCTTCT

est. tm =  7.4 oC

DeltaG @37 degrees =  -3.82 kcal/mole

 

                    3'-GAAAGGAAGACGTAGGAACG-5'

                       |||x||||||

          5'-AGCCTCCTACCTTGCCTTCT-3'

 

TTTA062-F AGCCTCCTACCTTGCCTTCT versus GATA68A03-F TGATTACTCTGAGGAAGAAGGC

Matches = 7

Score = 7

GCCTTCT

est. tm =  10.7 oC

DeltaG @37 degrees =  -5.00 kcal/mole

 

                    3'-CGGAAGAAGGAGTCTCATTAGT-5'

                       |||||||

       5'-AGCCTCCTACCTTGCCTTCT-3'

 

TTTA062-F AGCCTCCTACCTTGCCTTCT versus GGAA2F11N-F AAGATACACAGGAAGAAAGAAAGG

Matches = 9

Score = 7

CCTTNCNTTCT

est. tm =  less than zero

DeltaG @37 degrees =  -1.85 kcal/mole

 

                    3'-GGAAAGAAAGAAGGACACATAGAA-5'

                       ||||x|x||||

           5'-AGCCTCCTACCTTGCCTTCT-3'

 

TTTA062-F AGCCTCCTACCTTGCCTTCT versus GATA31E08-F AGGGGAGAAGGCTAGAATGA

Matches = 10

Score = 7

ANGGNAGNAGGCT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.83 kcal/mole

 

                    5'-AGGGGAGAAGGCTAGAATGA-3'

                       |x||x||x|||||

             3'-TCTTCCGTTCCATCCTCCGA-5'

 

TTTA062-F AGCCTCCTACCTTGCCTTCT versus GATA6B09-R ACATCTGAGACTTGTAGTAGAAGGC

Matches = 7

Score = 7

GCCTTCT

est. tm =  10.7 oC

DeltaG @37 degrees =  -5.00 kcal/mole

 

                    3'-CGGAAGATGATGTTCAGAGTCTACA-5'

                       |||||||

       5'-AGCCTCCTACCTTGCCTTCT-3'

 

GATA64H04-F GATGATAGAGATGGCACATGA versus GATA30F10A-F CCAACTCTCATCTGTATTATCTATG

Matches = 14

Score = 7

NCANNTNNCATCTNTATNATC

est. tm =  0.3 oC

DeltaG @37 degrees =  -0.37 kcal/mole

 

                     5'-CCAACTCTCATCTGTATTATCTATG-3'

                        x||xx|xx|||||x|||x|||

                     3'-AGTACACGGTAGAGATAGTAG-5'

 

GATA30F10A-F CCAACTCTCATCTGTATTATCTATG versus GATA6B09-R ACATCTGAGACTTGTAGTAGAAGGC

Matches = 11

Score = 7

ACANNTGAGANTTGN

est. tm =  13.8 oC

DeltaG @37 degrees =  -4.31 kcal/mole

 

                         5'-ACATCTGAGACTTGTAGTAGAAGGC-3'

                            |||xx|||||x|||x

               3'-GTATCTATTATGTCTACTCTCAACC-5'

 

GATA81D05-R CTCAAGTCCCAAAAATGAGG versus GATA31E10-F TATATTTTACACATTTTTGGGCC

Matches = 12

Score = 9

GNCCCAAAAATGNGN

est. tm =  21.0 oC

DeltaG @37 degrees =  -5.52 kcal/mole

 

                    3'-CCGGGTTTTTACACATTTTATAT-5'

                       |x||||||||||x|x

               5'-CTCAAGTCCCAAAAATGAGG-3'

 

GATA8C04-R TAAAATAGAAAGTGCCCCTCC versus GATA31E10-F TATATTTTACACATTTTTGGGCC

Matches = 8

Score = 7

TNTATTTTA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.12 kcal/mole

 

                     5'-TATATTTTACACATTTTTGGGCC-3'

                        |x|||||||

         3'-CCTCCCCGTGAAAGATAAAAT-5'

 

CATC015-R TCCAACAGTCCCTGGAACAC versus ATA22E01-R AGGACTGTAGGATTCCAAAGG

Matches = 10

Score = 8

NGGACTGTNGGA

est. tm =  24.4 oC

DeltaG @37 degrees =  -6.73 kcal/mole

 

                    5'-AGGACTGTAGGATTCCAAAGG-3'

                       x|||||||x|||

            3'-CACAAGGTCCCTGACAACCT-5'

 

CATC015-R TCCAACAGTCCCTGGAACAC versus GATA84E11-F TGTTGGAAGACTTCTCAGCC

Matches = 7

Score = 7

TGTTGGA

est. tm =  6.7 oC

DeltaG @37 degrees =  -4.50 kcal/mole

 

                    5'-TGTTGGAAGACTTCTCAGCC-3'

                       |||||||

       3'-CACAAGGTCCCTGACAACCT-5'

 

CATC015-R TCCAACAGTCCCTGGAACAC versus GATA48D07-R TGTAACAAGGGCTATGGGAA

Matches = 13

Score = 7

TGTNNCANGGNCTNTNGGA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.83 kcal/mole

 

                    5'-TGTAACAAGGGCTATGGGAA-3'

                       |||xx||x||x||x|x|||

                   3'-CACAAGGTCCCTGACAACCT-5'

 

CATC015-R TCCAACAGTCCCTGGAACAC versus GGAA2A03-R TAGGTTTTTAAGGAACAGGTGG

Matches = 13

Score = 7

CCANCNGTNCCTNNAANAC

est. tm =  less than zero

DeltaG @37 degrees =  -0.64 kcal/mole

 

                    3'-GGTGGACAAGGAATTTTTGGAT-5'

                       |||x|x||x|||xx||x||

                   5'-TCCAACAGTCCCTGGAACAC-3'

 

GATA27E01-R AAGGAGGAAAGCTTTTTGGA versus GATA72H07-R AGCTCACCTCCTCCAAGAAG

Matches = 8

Score = 7

CTTNTTGGA

est. tm =  less than zero

DeltaG @37 degrees =  -2.99 kcal/mole

 

                    3'-GAAGAACCTCCTCCACTCGA-5'

                       |||x|||||

         5'-AAGGAGGAAAGCTTTTTGGA-3'

 

GATA27E01-R AAGGAGGAAAGCTTTTTGGA versus GATA72H07-R AGCTCACCTCCTCCAAGAAG

Matches = 10

Score = 7

AGCTNNCCTCCTN

est. tm =  25.0 oC

DeltaG @37 degrees =  -6.86 kcal/mole

 

                    5'-AGCTCACCTCCTCCAAGAAG-3'

                       ||||xx||||||x

             3'-AGGTTTTTCGAAAGGAGGAA-5'

 

GATA21D04-F TAGGTTCTGGGCATGTCTGT versus ATA47D07-R TCAGATGACAGACAAACTCAGG

Matches = 11

Score = 7

NCTGNGNNTGTCTGT

est. tm =  13.7 oC

DeltaG @37 degrees =  -4.32 kcal/mole

 

                    3'-GGACTCAAACAGACAGTAGACT-5'

                       x|||x|xx|||||||

               5'-TAGGTTCTGGGCATGTCTGT-3'

 

ATA47D07-R TCAGATGACAGACAAACTCAGG versus AGAT120-R TTGTCTGTCATCTATCTACCCATC

Matches = 13

Score = 11

TTGTCTGTCATCTNN

est. tm =  39.2 oC

DeltaG @37 degrees =  -10.04 kcal/mole

 

                      5'-TTGTCTGTCATCTATCTACCCATC-3'

                         |||||||||||||xx

               3'-GGACTCAAACAGACAGTAGACT-5'

 

ATA78D02N-R GGCAAGAGAACTCACGTAA versus GATA129H04-R GGTCTCTTACCTGGCAGACA

Matches = 9

Score = 7

GNTCTCTTNCC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.21 kcal/mole

 

                   5'-GGTCTCTTACCTGGCAGACA-3'

                      |x||||||x||

           3'-AATGCACTCAAGAGAACGG-5'

 

GATA129H04-R GGTCTCTTACCTGGCAGACA versus GATA177C03N-R GCTGTAAGAGACCTGTGTTG

Matches = 10

Score = 7

NNNGTAAGAGACC

est. tm =  21.8 oC

DeltaG @37 degrees =  -5.72 kcal/mole

 

                    5'-GCTGTAAGAGACCTGTGTTG-3'

                       xxx||||||||||

             3'-ACAGACGGTCCATTCTCTGG-5'

 

GATA129H04-R GGTCTCTTACCTGGCAGACA versus GATA12H10-F ATGTAGACTGCTAACAGTGTCTGC

Matches = 7

Score = 7

GCAGACA

est. tm =  12.8 oC

DeltaG @37 degrees =  -5.24 kcal/mole

 

                    3'-CGTCTGTGACAATCGTCAGATGTA-5'

                       |||||||

       5'-GGTCTCTTACCTGGCAGACA-3'

 

GATA129H04-R GGTCTCTTACCTGGCAGACA versus GATA71H05-R GACATTGTCAGTGTCAGCCA

Matches = 8

Score = 7

TGGCNGACA

est. tm =  9.0 oC

DeltaG @37 degrees =  -4.76 kcal/mole

 

                    3'-ACCGACTGTGACTGTTACAG-5'

                       ||||x||||

         5'-GGTCTCTTACCTGGCAGACA-3'

 

GATA109-R GACCCTGTCTCAAAAAAAACA versus GATA6C01-F TCTAGGATGTTTTTAGAGAAAGGC

Matches = 13

Score = 7

NCCTNTCTCNAAAAANANN

est. tm =  13.8 oC

DeltaG @37 degrees =  -3.74 kcal/mole

 

                     3'-CGGAAAGAGATTTTTGTAGGATCT-5'

                        x|||x||||x|||||x|xx

                   5'-GACCCTGTCTCAAAAAAAACA-3'

 

GATA109-R GACCCTGTCTCAAAAAAAACA versus GATA81B01-R AAGACAGTGTCTAAGCTGTCACA

Matches = 9

Score = 7

NAGACAGNGTC

est. tm =  13.3 oC

DeltaG @37 degrees =  -4.70 kcal/mole

 

                     5'-AAGACAGTGTCTAAGCTGTCACA-3'

                        x||||||x|||

           3'-ACAAAAAAAACTCTGTCCCAG-5'

 

GATA4E11-R CAGACACAAATGCACACACA versus GATA124C08N-R ATGTGTGTGCATCTGTCTCTCTCA

Matches = 17

Score = 14

TGTGTGTGCATNTGTNTCTN

est. tm =  46.6 oC

DeltaG @37 degrees =  -12.82 kcal/mole

 

                    5'-ATGTGTGTGCATCTGTCTCTCTCA-3'

                        |||||||||||x|||x|||x

                     3'-ACACACACGTAAACACAGAC-5'

 

GATA124C08N-R ATGTGTGTGCATCTGTCTCTCTCA versus GATA23F08-R TATATTTCTTGGGAAAGATAGATGG

Matches = 12

Score = 8

NCATCTNTCTNTCNCA

est. tm =  8.2 oC

DeltaG @37 degrees =  -3.18 kcal/mole

 

                        3'-GGTAGATAGAAAGGGTTCTTTATAT-5'

                           x|||||x|||x||x||

                5'-ATGTGTGTGCATCTGTCTCTCTCA-3'

 

GATA13G11-R GGGTTCTGCAGAGAAACAGA versus GATA124C08N-R ATGTGTGTGCATCTGTCTCTCTCA

Matches = 10

Score = 7

NNAGAGANACAGA

est. tm =  15.7 oC

DeltaG @37 degrees =  -4.83 kcal/mole

 

                    3'-ACTCTCTCTGTCTACGTGTGTGTA-5'

                       xx|||||x|||||

             5'-GGGTTCTGCAGAGAAACAGA-3'

 

GATA198B05N-R GAGAGACAGAACCTATAGCATGC versus GATA124C08N-R ATGTGTGTGCATCTGTCTCTCTCA

Matches = 14

Score = 7

ATGNNNNNGNNTCTGTCTCTC

est. tm =  17.2 oC

DeltaG @37 degrees =  -3.66 kcal/mole

 

                       5'-ATGTGTGTGCATCTGTCTCTCTCA-3'

                          |||xxxxx|xx||||||||||

                     3'-CGTACGATATCCAAGACAGAGAG-5'

 

GATA165G02N-F CAGGATTTTTCAGAAAGACA versus GATA124C08N-R ATGTGTGTGCATCTGTCTCTCTCA

Matches = 9

Score = 7

TNAGANAGACA

est. tm =  less than zero

DeltaG @37 degrees =  -2.16 kcal/mole

 

                    3'-ACTCTCTCTGTCTACGTGTGTGTA-5'

                       |x|||x|||||

           5'-CAGGATTTTTCAGAAAGACA-3'

 

GATA22G12-R GGGTGACAGAGTGAGATGTG versus GATA124C08N-R ATGTGTGTGCATCTGTCTCTCTCA

Matches = 12

Score = 7

TGANAGAGNNAGATGNN

est. tm =  15.1 oC

DeltaG @37 degrees =  -4.19 kcal/mole

 

                    3'-ACTCTCTCTGTCTACGTGTGTGTA-5'

                       |||x||||xx|||||xx

                 5'-GGGTGACAGAGTGAGATGTG-3'

 

GATA84E11-R CACACATGCTGTGTCTCTCA versus GATA124C08N-R ATGTGTGTGCATCTGTCTCTCTCA

Matches = 7

Score = 7

ATGTGTG

est. tm =  6.3 oC

DeltaG @37 degrees =  -4.19 kcal/mole

 

                    5'-ATGTGTGTGCATCTGTCTCTCTCA-3'

                       |||||||

       3'-ACTCTCTGTGTCGTACACAC-5'

 

ATA25E07-R TTTTGGATTCGAGGTTACCA versus GATA24D12-R ACCCAGGATTGTGCTAACCT

Matches = 8

Score = 7

AGGTTANCA

est. tm =  less than zero

DeltaG @37 degrees =  -2.65 kcal/mole

 

                    3'-TCCAATCGTGTTAGGACCCA-5'

                       ||||||x||

         5'-TTTTGGATTCGAGGTTACCA-3'

 

ATA25E07-R TTTTGGATTCGAGGTTACCA versus GATA81D05-R CTCAAGTCCCAAAAATGAGG

Matches = 10

Score = 7

CTCNANTCCNAAA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.92 kcal/mole

 

                    5'-CTCAAGTCCCAAAAATGAGG-3'

                       |||x|x|||x|||

             3'-ACCATTGGAGCTTAGGTTTT-5'

 

GGAA5F09-R ACCATGGTACTCAGCAGTGC versus GGAA5F09-R ACCATGGTACTCAGCAGTGC

Matches = 8

Score = 8

ACCATGGT

est. tm =  20.7 oC

DeltaG @37 degrees =  -5.61 kcal/mole

 

                    5'-ACCATGGTACTCAGCAGTGC-3'

                       ||||||||

        3'-CGTGACGACTCATGGTACCA-5'

 

UT721-F TTGGTCTCCTCTGATGGGT versus TATC028-R AGCACCAACCACCATAGGAG

Matches = 11

Score = 8

CTCCTNTGNTGGNT

est. tm =  5.4 oC

DeltaG @37 degrees =  -3.33 kcal/mole

 

                   3'-GAGGATACCACCAACCACGA-5'

                      |||||x||x|||x|

              5'-TTGGTCTCCTCTGATGGGT-3'

 

TATC028-R AGCACCAACCACCATAGGAG versus GATA87E02N-F GTTGTTGCTGGGCTTAGAAA

Matches = 8

Score = 7

GTTGNTGCT

est. tm =  5.7 oC

DeltaG @37 degrees =  -3.98 kcal/mole

 

                    5'-GTTGTTGCTGGGCTTAGAAA-3'

                       ||||x||||

         3'-GAGGATACCACCAACCACGA-5'

 

GATA7C01-R AAAAAGAGTGTGGGCAACTG versus Mfd232-R GAAACACATCATTTACCCAC

Matches = 9

Score = 7

GTGGGNAANTG

est. tm =  1.9 oC

DeltaG @37 degrees =  -3.21 kcal/mole

 

                    3'-CACCCATTTACTACACAAAG-5'

                       |||||x||x||

           5'-AAAAAGAGTGTGGGCAACTG-3'

 

GATA7C01-R AAAAAGAGTGTGGGCAACTG versus AAT269-R CCCAGTGCCCATACACTTTT

Matches = 14

Score = 9

AAAAGNGTNTGGGCANNNG

est. tm =  21.5 oC

DeltaG @37 degrees =  -5.25 kcal/mole

 

                    3'-TTTTCACATACCCGTGACCC-5'

                       |||||x||x||||||xxx|

                   5'-AAAAAGAGTGTGGGCAACTG-3'

 

GATA7C01-R AAAAAGAGTGTGGGCAACTG versus ATA27F01-R CCCCACCCCTTTTAGTTTTA

Matches = 10

Score = 7

CCNCACNCNTTTT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.93 kcal/mole

 

                    5'-CCCCACCCCTTTTAGTTTTA-3'

                       ||x|||x|x||||

             3'-GTCAACGGGTGTGAGAAAAA-5'

 

GATA7C01-R AAAAAGAGTGTGGGCAACTG versus SDF1-R GAACAGGCAAAGATGCCCACTCTC

Matches = 12

Score = 9

GAGNGTGGGCANCTN

est. tm =  32.0 oC

DeltaG @37 degrees =  -8.16 kcal/mole

 

                    3'-CTCTCACCCGTAGAAACGGACAAG-5'

                       |||x|||||||x||x

               5'-AAAAAGAGTGTGGGCAACTG-3'

 

GATA124F08-R GAGAAAGAAGCCCCAAGAAC versus GATA8A05-F TGGTTCTGCTTTTTCTCTCC

Matches = 11

Score = 7

TGGNNCTNCTTTNTC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.39 kcal/mole

 

                    5'-TGGTTCTGCTTTTTCTCTCC-3'

                       |||xx||x||||x||

               3'-CAAGAACCCCGAAGAAAGAG-5'

 

GATA124F08-R GAGAAAGAAGCCCCAAGAAC versus ATA41E04-F TGTGATTCAGTAGGTCTTGGG

Matches = 8

Score = 7

CCCAAGANC

est. tm =  5.0 oC

DeltaG @37 degrees =  -3.86 kcal/mole

 

                    3'-GGGTTCTGGATGACTTAGTGT-5'

                       |||||||x|

         5'-GAGAAAGAAGCCCCAAGAAC-3'

 

GATA124F08-R GAGAAAGAAGCCCCAAGAAC versus GATA152F05-R TTCTTTGTCTGTGTGCGTGT

Matches = 8

Score = 7

TTCTTTNTC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.03 kcal/mole

 

                    5'-TTCTTTGTCTGTGTGCGTGT-3'

                       ||||||x||

         3'-CAAGAACCCCGAAGAAAGAG-5'

 

GATA64D02-F TATTTCAAAGGCAGCAAAGC versus GATA22D12-R TTTGATGAGCTTGAACTAGGG

Matches = 13

Score = 8

TTTGNTGNNNTTGAANTA

est. tm =  2.9 oC

DeltaG @37 degrees =  -1.22 kcal/mole

 

                    5'-TTTGATGAGCTTGAACTAGGG-3'

                       ||||x||xxx|||||x||

                  3'-CGAAACGACGGAAACTTTAT-5'

 

GATA11E09-R CATCCTTCCTGGTCCCTAGT versus GATA22D12-R TTTGATGAGCTTGAACTAGGG

Matches = 7

Score = 7

CCCTAGT

est. tm =  5.5 oC

DeltaG @37 degrees =  -4.31 kcal/mole

 

                    3'-GGGATCAAGTTCGAGTAGTTT-5'

                       |||||||

       5'-CATCCTTCCTGGTCCCTAGT-3'

 

TTTA049-R GGGTGACAGAGCAAGACTCC versus GATA73E11-F TTGGTGGTGTCATCCTCTTT

Matches = 11

Score = 7

ANAGAGNANGACNCC

est. tm =  less than zero

DeltaG @37 degrees =  -0.67 kcal/mole

 

                    3'-TTTCTCCTACTGTGGTGGTT-5'

                       |x||||x|x|||x||

               5'-GGGTGACAGAGCAAGACTCC-3'

 

TTTA049-R GGGTGACAGAGCAAGACTCC versus GATA30G01-R AATGAGGTCTTACTTTGTTGCC

Matches = 13

Score = 7

GGNNACANAGNAAGACNNC

est. tm =  1.9 oC

DeltaG @37 degrees =  -1.41 kcal/mole

 

                    3'-CCGTTGTTTCATTCTGGAGTAA-5'

                       ||xx|||x||x|||||xx|

                   5'-GGGTGACAGAGCAAGACTCC-3'

 

ATA29A06N-R ACCACACACAGCTACTTTTGC versus GATA72G11N-R CAAAATTAGCTGGGTATTGTCTAG

Matches = 16

Score = 12

CAAAANTAGCTGNGTNTNGT

est. tm =  22.2 oC

DeltaG @37 degrees =  -5.04 kcal/mole

 

                     5'-CAAAATTAGCTGGGTATTGTCTAG-3'

                        |||||x||||||x||x|x||

                    3'-CGTTTTCATCGACACACACCA-5'

 

GATA8F07-R GAACAGGATGGGGAAAACTT versus AAAAC001-F CCCCGAAATTTACACCATCA

Matches = 11

Score = 7

NGATGGNGNAAANTT

est. tm =  less than zero

DeltaG @37 degrees =  -1.67 kcal/mole

 

                    3'-ACTACCACATTTAAAGCCCC-5'

                       x|||||x|x|||x||

               5'-GAACAGGATGGGGAAAACTT-3'

 

GATA8F07-R GAACAGGATGGGGAAAACTT versus GATA43H01-F TAAAGGTTTATCCATGCTGTAGC

Matches = 14

Score = 8

AAGNTTTNNCCATNCTGTNN

est. tm =  16.6 oC

DeltaG @37 degrees =  -4.04 kcal/mole

 

                    5'-TAAAGGTTTATCCATGCTGTAGC-3'

                         |||x|||xx||||x||||xx

                      3'-TTCAAAAGGGGTAGGACAAG-5'

 

ATA47C04N-R AACATATCCTTCCCCTTCCA versus GTTTT001-F GGGAGGGAGTAGGAGGTGTT

Matches = 14

Score = 8

NGGANGGNGNAGGANNTGTT

est. tm =  15.5 oC

DeltaG @37 degrees =  -4.27 kcal/mole

 

                    5'-GGGAGGGAGTAGGAGGTGTT-3'

                       x|||x||x|x||||xx||||

                    3'-ACCTTCCCCTTCCTATACAA-5'

 

ATA47C04N-R AACATATCCTTCCCCTTCCA versus GTTTT001-F GGGAGGGAGTAGGAGGTGTT

Matches = 14

Score = 8

AACANNTCCTNCNCCNTCCN

est. tm =  17.2 oC

DeltaG @37 degrees =  -4.67 kcal/mole

 

                    3'-TTGTGGAGGATGAGGGAGGG-5'

                       ||||xx||||x|x||x|||x

                    5'-AACATATCCTTCCCCTTCCA-3'

 

GATA128C02N-R AAGGAACTGTTAGGGGCCT versus ATA47C04N-R AACATATCCTTCCCCTTCCA

Matches = 9

Score = 7

AACANNTCCTT

est. tm =  4.8 oC

DeltaG @37 degrees =  -3.42 kcal/mole

 

                   5'-AACATATCCTTCCCCTTCCA-3'

                      ||||xx|||||

           3'-TCCGGGGATTGTCAAGGAA-5'

 

GATA66D01-R TAATTCAAATCTTTCCTGTCAGG versus GATA66D01-R TAATTCAAATCTTTCCTGTCAGG

Matches = 8

Score = 7

CCTGNCAGG

est. tm =  7.3 oC

DeltaG @37 degrees =  -4.28 kcal/mole

 

                       3'-GGACTGTCCTTTCTAAACTTAAT-5'

                          ||||x||||

         5'-TAATTCAAATCTTTCCTGTCAGG-3'

 

GATA88G05-R GAGTTTTATTGGCCAAAGCA versus ATA6C09-R AAAACTCAAGCTCAGAAAGGC

Matches = 7

Score = 7

AAAACTC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -3.13 kcal/mole

 

                    5'-AAAACTCAAGCTCAGAAAGGC-3'

                       |||||||

       3'-ACGAAACCGGTTATTTTGAG-5'

 

ATA78D02N-R GGCAAGAGAACTCACGTAA versus GATA88G05-R GAGTTTTATTGGCCAAAGCA

Matches = 10

Score = 7

GAGTTNTNTTGNC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.75 kcal/mole

 

                   5'-GAGTTTTATTGGCCAAAGCA-3'

                      |||||x|x|||x|

             3'-AATGCACTCAAGAGAACGG-5'

 

GATA88G05-R GAGTTTTATTGGCCAAAGCA versus GATA2H09-F TTGCCACTGAAAATCATATCC

Matches = 11

Score = 7

TNGCCANTNAAANTC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.05 kcal/mole

 

                    5'-TTGCCACTGAAAATCATATCC-3'

                       |x||||x|x|||x||

               3'-ACGAAACCGGTTATTTTGAG-5'

 

GATA88G05-R GAGTTTTATTGGCCAAAGCA versus GGAA3F06-R CCAAATAAGACTCAGGACGC

Matches = 10

Score = 7

NCNAATAANACTC

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.72 kcal/mole

 

                    5'-CCAAATAAGACTCAGGACGC-3'

                       x|x|||||x||||

             3'-ACGAAACCGGTTATTTTGAG-5'

 

GATA88G05-R GAGTTTTATTGGCCAAAGCA versus GATA73E11-R CTCTTAACTGATTTGGCCGA

Matches = 10

Score = 8

TNGGCCAAANCA

est. tm =  11.5 oC

DeltaG @37 degrees =  -4.60 kcal/mole

 

                    3'-AGCCGGTTTAGTCAATTCTC-5'

                       |x|||||||x||

            5'-GAGTTTTATTGGCCAAAGCA-3'

 

GATA4E11-R CAGACACAAATGCACACACA versus GATA50F11-F ATACATTTATATTTGCATCTGTGTG

Matches = 13

Score = 8

NACACANATGCANANANA

est. tm =  11.8 oC

DeltaG @37 degrees =  -3.76 kcal/mole

 

                    3'-GTGTGTCTACGTTTATATTTACATA-5'

                       x|||||x|||||x|x|x|

                  5'-CAGACACAAATGCACACACA-3'

 

GATA4E11-R CAGACACAAATGCACACACA versus GATA152F05-R TTCTTTGTCTGTGTGCGTGT

Matches = 10

Score = 7

ANANGCACACANA

est. tm =  less than zero

DeltaG @37 degrees =  -2.60 kcal/mole

 

                    3'-TGTGCGTGTGTCTGTTTCTT-5'

                       |x|x|||||||x|

             5'-CAGACACAAATGCACACACA-3'

 

GATA4E11-R CAGACACAAATGCACACACA versus GATA11C06N-R AGCGTTTGTGTGTGCATCTGT

Matches = 11

Score = 8

NGCNTTTGTGTNTG

est. tm =  19.7 oC

DeltaG @37 degrees =  -5.44 kcal/mole

 

                    5'-AGCGTTTGTGTGTGCATCTGT-3'

                       x||x|||||||x||

              3'-ACACACACGTAAACACAGAC-5'

 

GATA4E11-R CAGACACAAATGCACACACA versus GATA11C06N-R AGCGTTTGTGTGTGCATCTGT

Matches = 14

Score = 13

ACANATGCACACACA

est. tm =  42.3 oC

DeltaG @37 degrees =  -11.00 kcal/mole

 

                    3'-TGTCTACGTGTGTGTTTGCGA-5'

                       |||x|||||||||||

               5'-CAGACACAAATGCACACACA-3'

 

GATA4E11-R CAGACACAAATGCACACACA versus GATA165B12-R TTAAAATCATTTTCACTGTGTATGC

Matches = 8

Score = 7

GCANACACA

est. tm =  9.6 oC

DeltaG @37 degrees =  -4.59 kcal/mole

 

                    3'-CGTATGTGTCACTTTTACTAAAATT-5'

                       |||x|||||

         5'-CAGACACAAATGCACACACA-3'

 

GATA4D07-R CTCCATGATCCAGTCACCTC versus GATA68H04-R CCTGGATCATGAATTGCTAT

Matches = 11

Score = 8

NCTGGATCATGNAN

est. tm =  27.6 oC

DeltaG @37 degrees =  -7.13 kcal/mole

 

                    5'-CCTGGATCATGAATTGCTAT-3'

                       x||||||||||x|x

              3'-CTCCACTGACCTAGTACCTC-5'

 

GATA4D07-R CTCCATGATCCAGTCACCTC versus GGAA7G08NZ-R CTGGGCAATGGAGTGAGACT

Matches = 10

Score = 7

CTGGNNNATGGAG

est. tm =  14.8 oC

DeltaG @37 degrees =  -4.54 kcal/mole

 

                    5'-CTGGGCAATGGAGTGAGACT-3'

                       ||||xxx||||||

             3'-CTCCACTGACCTAGTACCTC-5'

 

ATA27H09-R GAGCAGGATTTGTAACCCTG versus ATA27H09-R GAGCAGGATTTGTAACCCTG

Matches = 12

Score = 7

CAGGNTTNNNAANCCTG

est. tm =  6.0 oC

DeltaG @37 degrees =  -2.46 kcal/mole

 

                    3'-GTCCCAATGTTTAGGACGAG-5'

                       ||||x||xxx||x||||

                 5'-GAGCAGGATTTGTAACCCTG-3'

 

ATA27H09-R GAGCAGGATTTGTAACCCTG versus ATT077-F CACTGCCCCAGTGATACAAA

Matches = 10

Score = 8

TTTGTANCNCTG

est. tm =  less than zero

DeltaG @37 degrees =  -2.11 kcal/mole

 

                    3'-AAACATAGTGACCCCGTCAC-5'

                       ||||||x|x|||

            5'-GAGCAGGATTTGTAACCCTG-3'

 

ATA27H09-R GAGCAGGATTTGTAACCCTG versus GATA6E05-R GTTCTTCAGGGAAACAGAACC

Matches = 11

Score = 7

GGNTNTGTNNCCCTG

est. tm =  6.8 oC

DeltaG @37 degrees =  -3.39 kcal/mole

 

                    3'-CCAAGACAAAGGGACTTCTTG-5'

                       ||x|x|||xx|||||

               5'-GAGCAGGATTTGTAACCCTG-3'

 

GATA7F05-R CTTACCTGGGTCTCCAGCTT versus GATA65C03N-R TAAGAACAAGTAAAAAAAGCTGG

Matches = 7

Score = 7

CCAGCTT

est. tm =  11.6 oC

DeltaG @37 degrees =  -5.14 kcal/mole

 

                    3'-GGTCGAAAAAAATGAACAAGAAT-5'

                       |||||||

       5'-CTTACCTGGGTCTCCAGCTT-3'

 

GATA29A05-F TTTTGTTTCTTGATCTGGGC versus GATA65C03N-R TAAGAACAAGTAAAAAAAGCTGG

Matches = 13

Score = 8

NNAGANCAAGNAANAAAA

est. tm =  7.7 oC

DeltaG @37 degrees =  -2.33 kcal/mole

 

                    5'-TAAGAACAAGTAAAAAAAGCTGG-3'

                       xx|||x||||x||x||||

                  3'-CGGGTCTAGTTCTTTGTTTT-5'

 

GATA29F06Z-R ACACCTGCCTTTCTTTCCTG versus GATA65C03N-R TAAGAACAAGTAAAAAAAGCTGG

Matches = 12

Score = 7

CCNGCNTTTNTTNCNTG

est. tm =  less than zero

DeltaG @37 degrees =  greater than zero

 

                    3'-GGTCGAAAAAAATGAACAAGAAT-5'

                       ||x||x|||x||x|x||

                 5'-ACACCTGCCTTTCTTTCCTG-3'

 

GATA65C03N-R TAAGAACAAGTAAAAAAAGCTGG versus GATA144D04-R ATCTTTCTGTACTTGCAGTGAGG

Matches = 14

Score = 7

ANCTTTNTNTACTTGNNNTNA

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                       5'-ATCTTTCTGTACTTGCAGTGAGG-3'

                          |x||||x|x||||||xxx|x|

                     3'-GGTCGAAAAAAATGAACAAGAAT-5'

 

GATA30E06-R GGGATGGATGGAAAAGATTC versus GATA6B09-F TTGCATCCATACGGAAAGTC

Matches = 10

Score = 7

TTNCATCCATNCN

est. tm =  5.0 oC

DeltaG @37 degrees =  -3.34 kcal/mole

 

                    5'-TTGCATCCATACGGAAAGTC-3'

                       ||x|||||||x|x

             3'-CTTAGAAAAGGTAGGTAGGG-5'

 

GATA30E06-R GGGATGGATGGAAAAGATTC versus GATA23B01N-R TTCCTAAGCATGAATTTTTACC

Matches = 9

Score = 7

GGNAAANATTC

est. tm =  less than zero

DeltaG @37 degrees =  -1.23 kcal/mole

 

                    3'-CCATTTTTAAGTACGAATCCTT-5'

                       ||x|||x||||

           5'-GGGATGGATGGAAAAGATTC-3'

 

GATA30E06-R GGGATGGATGGAAAAGATTC versus GGAT3F08-R TATCCATCCATCCATCAACC

Matches = 12

Score = 10

TNTCCATCCATCCN

est. tm =  34.4 oC

DeltaG @37 degrees =  -8.73 kcal/mole

 

                    5'-TATCCATCCATCCATCAACC-3'

                       |x|||||||||||x

              3'-CTTAGAAAAGGTAGGTAGGG-5'

 

GATA30E06-R GGGATGGATGGAAAAGATTC versus ATA28C05-R CCATCCCCTAAACCTCTCAT

Matches = 7

Score = 7

CCATCCC

est. tm =  12.2 oC

DeltaG @37 degrees =  -5.22 kcal/mole

 

                    5'-CCATCCCCTAAACCTCTCAT-3'

                       |||||||

       3'-CTTAGAAAAGGTAGGTAGGG-5'

 

GATA23D03N-R ATTCTTTGTCTCCCCAGTTG versus GATA3E10-R GGGGGAAAAAGAACACTGTA

Matches = 11

Score = 8

GGGNGANAAAGAAN

est. tm =  16.5 oC

DeltaG @37 degrees =  -4.91 kcal/mole

 

                    5'-GGGGGAAAAAGAACACTGTA-3'

                       |||x||x||||||x

              3'-GTTGACCCCTCTGTTTCTTA-5'

 

GATA23D03N-R ATTCTTTGTCTCCCCAGTTG versus ATA10H11-F GGTGACAGAGACTCTTGTCTCA

Matches = 10

Score = 7

GGNGACANAGANT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.80 kcal/mole

 

                    5'-GGTGACAGAGACTCTTGTCTCA-3'

                       ||x||||x|||x|

             3'-GTTGACCCCTCTGTTTCTTA-5'

 

GATA23D03N-R ATTCTTTGTCTCCCCAGTTG versus GGAA3F06-F CAGAACAAACAAATGGGGAG

Matches = 10

Score = 9

CTCCCCANTTG

est. tm =  24.1 oC

DeltaG @37 degrees =  -6.59 kcal/mole

 

                    3'-GAGGGGTAAACAAACAAGAC-5'

                       |||||||x|||

           5'-ATTCTTTGTCTCCCCAGTTG-3'

 

GATA23D03N-R ATTCTTTGTCTCCCCAGTTG versus GTAT1A05-R TTGGTGACAAAGAAAGTCTCC

Matches = 11

Score = 7

NNGGNGACAAAGAAN

est. tm =  24.2 oC

DeltaG @37 degrees =  -6.28 kcal/mole

 

                    5'-TTGGTGACAAAGAAAGTCTCC-3'

                       xx||x|||||||||x

               3'-GTTGACCCCTCTGTTTCTTA-5'

 

ATT015-R TCACACTCTGGGGACTGTTG versus GATA23A02-R GACAGCAGACTGTGAGACTTC

Matches = 11

Score = 7

GNCNNCAGANTGTGA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.93 kcal/mole

 

                    5'-GACAGCAGACTGTGAGACTTC-3'

                       |x|xx||||x|||||

               3'-GTTGTCAGGGGTCTCACACT-5'

 

GATA158H04-R CCAGTCTTCCGTCACTATGC versus GATA23A02-R GACAGCAGACTGTGAGACTTC

Matches = 10

Score = 7

GACNGNAGACTGN

est. tm =  10.7 oC

DeltaG @37 degrees =  -4.14 kcal/mole

 

                    5'-GACAGCAGACTGTGAGACTTC-3'

                       |||x|x||||||x

             3'-CGTATCACTGCCTTCTGACC-5'

 

AGAT021-R TCCTGAATGAGGGGACACTT versus GATA28F03-F TTCAGGAGTTTAGCTTTCTATGC

Matches = 7

Score = 7

TTCAGGA

est. tm =  4.4 oC

DeltaG @37 degrees =  -4.19 kcal/mole

 

                    5'-TTCAGGAGTTTAGCTTTCTATGC-3'

                       |||||||

       3'-TTCACAGGGGAGTAAGTCCT-5'

 

GATA29B01-R AATATGGAACCAGCCCAAAT versus GATA73D01-R GATACCATATTCAACATGAAGAGG

Matches = 9

Score = 7

GNTNCCATATT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.75 kcal/mole

 

                    5'-GATACCATATTCAACATGAAGAGG-3'

                       |x|x|||||||

           3'-TAAACCCGACCAAGGTATAA-5'

 

GATA8B05-R CACTGTGATTTGCTGTTGGA versus GATA72H07-F AAAGCACAGTCCACACTCCT

Matches = 9

Score = 7

AAANCACAGTN

est. tm =  12.2 oC

DeltaG @37 degrees =  -4.62 kcal/mole

 

                    5'-AAAGCACAGTCCACACTCCT-3'

                       |||x||||||x

           3'-AGGTTGTCGTTTAGTGTCAC-5'

 

GATA8B05-R CACTGTGATTTGCTGTTGGA versus ATGT006Z-R CTGCACAGCAGAGTGAGACC

Matches = 11

Score = 7

CNGCANANCANAGTG

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.59 kcal/mole

 

                    5'-CTGCACAGCAGAGTGAGACC-3'

                       |x|||x|x||x||||

               3'-AGGTTGTCGTTTAGTGTCAC-5'

 

GATA8B05-R CACTGTGATTTGCTGTTGGA versus GATA188F04-R TACAGTAAATCACTTGGTAGGAGA

Matches = 12

Score = 7

NACAGNAAATCACNNNG

est. tm =  13.5 oC

DeltaG @37 degrees =  -3.58 kcal/mole

 

                    5'-TACAGTAAATCACTTGGTAGGAGA-3'

                       x||||x|||||||xxx|

                 3'-AGGTTGTCGTTTAGTGTCAC-5'

 

GATA24H01N-R CATAATTGCTTGGGCAATTC versus GATA6F06-R ACCCAATTATGGTGTTGTTACC

Matches = 9

Score = 7

ANNCAATTATG

est. tm =  less than 0 oC

DeltaG @37 degrees =  -1.40 kcal/mole

 

                    5'-ACCCAATTATGGTGTTGTTACC-3'

                       |xx||||||||

           3'-CTTAACGGGTTCGTTAATAC-5'

 

ATA2A03-R ACACCATCATGGCGCATG versus GATA6F06-R ACCCAATTATGGTGTTGTTACC

Matches = 11

Score = 7

NCNCNATNATGGTGT

est. tm =  1.2 oC

DeltaG @37 degrees =  -2.55 kcal/mole

 

                  5'-ACCCAATTATGGTGTTGTTACC-3'

                     x|x|x||x|||||||

               3'-GTACGCGGTACTACCACA-5'

 

GATA6F06-R ACCCAATTATGGTGTTGTTACC versus GATA81D12-R TAATGTACCTGGTACCAAAAACA

Matches = 10

Score = 7

TGNTNTTGNTACC

est. tm =  less than zero

DeltaG @37 degrees =  -0.95 kcal/mole

 

                      3'-ACAAAAACCATGGTCCATGTAAT-5'

                         ||x|x|||x||||

             5'-ACCCAATTATGGTGTTGTTACC-3'

 

GATA68H04-F ACAAGAACAGCATGGGGTAA versus GATA6F06-R ACCCAATTATGGTGTTGTTACC

Matches = 13

Score = 8

ACCCNATNNTGNTNTTGT

est. tm =  3.2 oC

DeltaG @37 degrees =  -2.25 kcal/mole

 

                    5'-ACCCAATTATGGTGTTGTTACC-3'

                       ||||x||xx||x|x||||

                  3'-AATGGGGTACGACAAGAACA-5'

 

GATA104-F CCTCCATAATTGTGTGAGCC versus GATA6F06-R ACCCAATTATGGTGTTGTTACC

Matches = 12

Score = 9

ACNCAATTATGGNGN

est. tm =  16.3 oC

DeltaG @37 degrees =  -4.52 kcal/mole

 

                    5'-ACCCAATTATGGTGTTGTTACC-3'

                       ||x|||||||||x|x

               3'-CCGAGTGTGTTAATACCTCC-5'

 

UT7544-R CATAATAGGTTTGAGGTATCT versus GATA6F06-R ACCCAATTATGGTGTTGTTACC

Matches = 9

Score = 7

ANCCNATTATG

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.75 kcal/mole

 

                     5'-ACCCAATTATGGTGTTGTTACC-3'

                        |x||x||||||

           3'-TCTATGGAGTTTGGATAATAC-5'

 

GATA7F05-R CTTACCTGGGTCTCCAGCTT versus ATA25F04-R AGACCCAGTTGATGCAATGT

Matches = 10

Score = 7

AGACCCAGNTNAN

est. tm =  13.5 oC

DeltaG @37 degrees =  -4.97 kcal/mole

 

                    5'-AGACCCAGTTGATGCAATGT-3'

                       ||||||||x|x|x

             3'-TTCGACCTCTGGGTCCATTC-5'

 

GATA84B12-R ATGAGGACGGTTGACATCTG versus ACT1A01-F GTCCTCATCTGTAAAACGGG

Matches = 8

Score = 8

GTCCTCAT

est. tm =  15.1 oC

DeltaG @37 degrees =  -5.28 kcal/mole

 

                    5'-GTCCTCATCTGTAAAACGGG-3'

                       ||||||||

        3'-GTCTACAGTTGGCAGGAGTA-5'

 

GATA84B12-R ATGAGGACGGTTGACATCTG versus GATA27A03-R AGATGCCGCCTGTACTAATG

Matches = 13

Score = 7

AGATGNCNNCNGTNCTNAT

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                    5'-AGATGCCGCCTGTACTAATG-3'

                       |||||x|xx|x||x||x||

                   3'-GTCTACAGTTGGCAGGAGTA-5'

 

ATA34G06-R GTTGTTTGCCCTACAACCTG versus ATA34G06-R GTTGTTTGCCCTACAACCTG

Matches = 12

Score = 7

GTTGTNNGNCNNACAAC

est. tm =  5.2 oC

DeltaG @37 degrees =  -2.04 kcal/mole

 

                    5'-GTTGTTTGCCCTACAACCTG-3'

                       |||||xx|x|xx|||||

                 3'-GTCCAACATCCCGTTTGTTG-5'

 

GATA3C02-R CTGGCCCCATCTCTCTCTAT versus GATA4H03-R TCCAATGGAAAGAAATGGAG

Matches = 12

Score = 8

CNCCATNTCTNTCNAT

est. tm =  less than zero

DeltaG @37 degrees =  -0.85 kcal/mole

 

                    3'-GAGGTAAAGAAAGGTAACCT-5'

                       |x||||x|||x||x||

                5'-CTGGCCCCATCTCTCTCTAT-3'

 

GATA3C02-R CTGGCCCCATCTCTCTCTAT versus GATA23F05-F ATGGTATTAGGAGATGGGGC

Matches = 12

Score = 7

GCCCCATCTCNNNNTAN

est. tm =  28.8 oC

DeltaG @37 degrees =  -7.21 kcal/mole

 

                    3'-CGGGGTAGAGGATTATGGTA-5'

                       ||||||||||xxxx||x

                 5'-CTGGCCCCATCTCTCTCTAT-3'

 

GATA3C02-R CTGGCCCCATCTCTCTCTAT versus GATA48D07-F TGGACAACAGAGTGAGATGC

Matches = 11

Score = 8

NCATCTCNCTCTNT

est. tm =  11.5 oC

DeltaG @37 degrees =  -3.97 kcal/mole

 

                    3'-CGTAGAGTGAGACAACAGGT-5'

                       x||||||x||||x|

              5'-CTGGCCCCATCTCTCTCTAT-3'

 

GATA3C02-R CTGGCCCCATCTCTCTCTAT versus GATA22G12-R GGGTGACAGAGTGAGATGTG

Matches = 12

Score = 9

CNCATCTCNCTCTNT

est. tm =  6.1 oC

DeltaG @37 degrees =  -2.72 kcal/mole

 

                    3'-GTGTAGAGTGAGACAGTGGG-5'

                       |x||||||x||||x|

               5'-CTGGCCCCATCTCTCTCTAT-3'

 

GATA3C02-R CTGGCCCCATCTCTCTCTAT versus AAAT007-R AGGCTCATCCACAGAGATGG

Matches = 11

Score = 8

CCATCTCTNTNNAT

est. tm =  7.4 oC

DeltaG @37 degrees =  -3.10 kcal/mole

 

                    3'-GGTAGAGACACCTACTCGGA-5'

                       ||||||||x|xx||

              5'-CTGGCCCCATCTCTCTCTAT-3'

 

TTTA040-R GGGTGGAAAGAAGGGCTTTA versus GATA30F10A-R TCTTATCTCCACCCACCAGA

Matches = 11

Score = 8

NCTTNTNTCCACCC

est. tm =  21.0 oC

DeltaG @37 degrees =  -5.91 kcal/mole

 

                    5'-TCTTATCTCCACCCACCAGA-3'

                       x|||x|x|||||||

              3'-ATTTCGGGAAGAAAGGTGGG-5'

 

TTTA040-R GGGTGGAAAGAAGGGCTTTA versus GATA30F10-R TCTTATCTCCACCCACCAGA

Matches = 11

Score = 8

NCTTNTNTCCACCC

est. tm =  21.0 oC

DeltaG @37 degrees =  -5.91 kcal/mole

 

                    5'-TCTTATCTCCACCCACCAGA-3'

                       x|||x|x|||||||

              3'-ATTTCGGGAAGAAAGGTGGG-5'

 

ATA82B02N-R AGAGGCTGAGGTGAAGGATT versus 4PTEL04-R GGTCTAAATCAATGACCTAAGC

Matches = 12

Score = 8

GCTNAGGTNANNGATT

est. tm =  8.5 oC

DeltaG @37 degrees =  -3.19 kcal/mole

 

                    3'-CGAATCCAGTAACTAAATCTGG-5'

                       |||x||||x|xx||||

                5'-AGAGGCTGAGGTGAAGGATT-3'

 

AFMb035xb9-R CGTTCCAACTGTCACCAGC versus ATT015-R TCACACTCTGGGGACTGTTG

Matches = 11

Score = 8

CAACNGTCNCCAGN

est. tm =  19.8 oC

DeltaG @37 degrees =  -5.56 kcal/mole

 

                   3'-GTTGTCAGGGGTCTCACACT-5'

                      ||||x|||x||||x

              5'-CGTTCCAACTGTCACCAGC-3'

 

ATT015-R TCACACTCTGGGGACTGTTG versus GATA27-R AGTGTGAACATTATTTCATTCTGG

Matches = 7

Score = 7

AGTGTGA

est. tm =  6.3 oC

DeltaG @37 degrees =  -4.40 kcal/mole

 

                    5'-AGTGTGAACATTATTTCATTCTGG-3'

                       |||||||

       3'-GTTGTCAGGGGTCTCACACT-5'

 

ATT015-R TCACACTCTGGGGACTGTTG versus GATA64A09-R CAGAGTGAGACCCTGTTTCA

Matches = 9

Score = 8

CAGAGTGNGA

est. tm =  11.0 oC

DeltaG @37 degrees =  -4.36 kcal/mole

 

                    5'-CAGAGTGAGACCCTGTTTCA-3'

                       |||||||x||

          3'-GTTGTCAGGGGTCTCACACT-5'

 

ATT015-R TCACACTCTGGGGACTGTTG versus GATA26D02N-R AGTGTGATATGAATAATGAGCTGC

Matches = 7

Score = 7

AGTGTGA

est. tm =  6.3 oC

DeltaG @37 degrees =  -4.40 kcal/mole

 

                    5'-AGTGTGATATGAATAATGAGCTGC-3'

                       |||||||

       3'-GTTGTCAGGGGTCTCACACT-5'

 

ATT015-R TCACACTCTGGGGACTGTTG versus ATA26D07-F AGAGTGAGATTCTGTCTCAATTAA

Matches = 8

Score = 7

AGAGTGNGA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -3.13 kcal/mole

 

                    5'-AGAGTGAGATTCTGTCTCAATTAA-3'

                       ||||||x||

         3'-GTTGTCAGGGGTCTCACACT-5'

 

ATA27C07-R GCAACAAACCTGCACATTCT versus GATA107-R GTCTCTGCATTTGCTGGTTT

Matches = 11

Score = 7

AAACCNGCANATNCN

est. tm =  6.4 oC

DeltaG @37 degrees =  -3.43 kcal/mole

 

                    3'-TTTGGTCGTTTACGTCTCTG-5'

                       |||||x|||x||x|x

               5'-GCAACAAACCTGCACATTCT-3'

 

ATA27C07-R GCAACAAACCTGCACATTCT versus CTAT014-R CAGCTTTCAAACAGTGCAGGT

Matches = 10

Score = 7

ACCTGCACNNTNT

est. tm =  10.0 oC

DeltaG @37 degrees =  -4.26 kcal/mole

 

                    3'-TGGACGTGACAAACTTTCGAC-5'

                       ||||||||xx|x|

             5'-GCAACAAACCTGCACATTCT-3'

 

ATA27C07-R GCAACAAACCTGCACATTCT versus GATG011-F CTCAAAGCCTGTGGAGGTCT

Matches = 11

Score = 7

ANACCTNCACANNCT

est. tm =  less than zero

DeltaG @37 degrees =  -2.35 kcal/mole

 

                    3'-TCTGGAGGTGTCCGAAACTC-5'

                       |x||||x||||xx||

               5'-GCAACAAACCTGCACATTCT-3'

 

ATA27C07-R GCAACAAACCTGCACATTCT versus GATA32B03-R AGGTATGGTGCCACACACTT

Matches = 9

Score = 7

AGGTNTGNTGC

est. tm =  4.8 oC

DeltaG @37 degrees =  -3.83 kcal/mole

 

                    5'-AGGTATGGTGCCACACACTT-3'

                       ||||x||x|||

           3'-TCTTACACGTCCAAACAACG-5'

 

ATA2A03-R ACACCATCATGGCGCATG versus GATA104-F CCTCCATAATTGTGTGAGCC

Matches = 11

Score = 7

NCNCCATNATNGTGT

est. tm =  1.2 oC

DeltaG @37 degrees =  -2.55 kcal/mole

 

                  5'-CCTCCATAATTGTGTGAGCC-3'

                     x|x||||x||x||||

               3'-GTACGCGGTACTACCACA-5'

 

ATA2A03-R ACACCATCATGGCGCATG versus AAAT007-F GCCAACATGGTGAAAACCTAA

Matches = 10

Score = 7

GCCANNATGGTGN

est. tm =  27.0 oC

DeltaG @37 degrees =  -7.17 kcal/mole

 

                  5'-GCCAACATGGTGAAAACCTAA-3'

                     ||||xx||||||x

             3'-GTACGCGGTACTACCACA-5'

 

GATA62A12-R GCCAGATACATGGCTAAGGA versus GATA90G11-R ATCTGGCAATACTTCGCAAG

Matches = 7

Score = 7

ATCTGGC

est. tm =  10.8 oC

DeltaG @37 degrees =  -5.03 kcal/mole

 

                    5'-ATCTGGCAATACTTCGCAAG-3'

                       |||||||

       3'-AGGAATCGGTACATAGACCG-5'

 

GATA107-R GTCTCTGCATTTGCTGGTTT versus GGAA3F06-F CAGAACAAACAAATGGGGAG

Matches = 13

Score = 8

CTCNNCATTTGNTNGTTN

est. tm =  12.3 oC

DeltaG @37 degrees =  -3.26 kcal/mole

 

                    3'-GAGGGGTAAACAAACAAGAC-5'

                       |||xx||||||x|x|||x

                  5'-GTCTCTGCATTTGCTGGTTT-3'

 

GATA21C12-F AAACAATCAAAGGCCCAGG versus GATA107-R GTCTCTGCATTTGCTGGTTT

Matches = 13

Score = 7

AAACNANCAAANGCNNAGN

est. tm =  4.4 oC

DeltaG @37 degrees =  -2.12 kcal/mole

 

                   3'-TTTGGTCGTTTACGTCTCTG-5'

                      ||||x|x||||x||xx||x

                   5'-AAACAATCAAAGGCCCAGG-3'

 

GATA27G03-R AAGGGATTTTAGGAACTGATACG versus GATA41G07-R AAAATCCTTTCAACAAATAGAAGC

Matches = 9

Score = 8

AAAATCCNTT

est. tm =  1.0 oC

DeltaG @37 degrees =  -2.80 kcal/mole

 

                       5'-AAAATCCTTTCAACAAATAGAAGC-3'

                          |||||||x||

          3'-GCATAGTCAAGGATTTTAGGGAA-5'

 

GATA128C02N-F AGACCCCAAAATTACTTGAG versus GATA27G03-R AAGGGATTTTAGGAACTGATACG

Matches = 12

Score = 7

AAGNNATTTTNGGNNCT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.49 kcal/mole

 

                    5'-AAGGGATTTTAGGAACTGATACG-3'

                       |||xx|||||x||xx||

                 3'-GAGTTCATTAAAACCCCAGA-5'

 

GATA31A10-F TCTAAACTCCTTTGCACAGTC versus GATA27G03-R AAGGGATTTTAGGAACTGATACG

Matches = 10

Score = 7

AANGGANTTTAGN

est. tm =  1.1 oC

DeltaG @37 degrees =  -2.58 kcal/mole

 

                     5'-AAGGGATTTTAGGAACTGATACG-3'

                        ||x|||x|||||x

             3'-CTGACACGTTTCCTCAAATCT-5'

 

GATA42H02-R AAAGCCAAAAGATGATGCAA versus GATA29A01-F CTGTGCCTCTATTGCCTCAT

Matches = 8

Score = 7

ATGANGCAA

est. tm =  0.6 oC

DeltaG @37 degrees =  -3.36 kcal/mole

 

                    3'-TACTCCGTTATCTCCGTGTC-5'

                       ||||x||||

         5'-AAAGCCAAAAGATGATGCAA-3'

 

GATA42H02-R AAAGCCAAAAGATGATGCAA versus GATA73E11-F TTGGTGGTGTCATCCTCTTT

Matches = 10

Score = 7

AAAGANGATGNNA

est. tm =  less than zero

DeltaG @37 degrees =  -1.36 kcal/mole

 

                    3'-TTTCTCCTACTGTGGTGGTT-5'

                       |||||x||||xx|

             5'-AAAGCCAAAAGATGATGCAA-3'

 

GATA62A04-R TAAGGCATGTCTCGGAGCTA versus GATA5B02-R CAGACATTCTTTATTCTTTACCTCC

Matches = 10

Score = 7

NAGACATNCNTTA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.11 kcal/mole

 

                    5'-CAGACATTCTTTATTCTTTACCTCC-3'

                       x||||||x|x|||

             3'-ATCGAGGCTCTGTACGGAAT-5'

 

GATA145D10N-R TTGAGCCCAGAAGTTTGAG versus GATA46A12-F CTTGTGGGCTGTAGTTTGCT

Matches = 10

Score = 7

CTTNTGGGCTNNA

est. tm =  9.4 oC

DeltaG @37 degrees =  -4.08 kcal/mole

 

                   5'-CTTGTGGGCTGTAGTTTGCT-3'

                      |||x||||||xx|

             3'-GAGTTTGAAGACCCGAGTT-5'

 

GATA3E10-R GGGGGAAAAAGAACACTGTA versus GGAA29H03N-R AGTTGACTCTTTCCCCAACTA

Matches = 12

Score = 7

AGTNNNCTNTTTCCCCN

est. tm =  20.1 oC

DeltaG @37 degrees =  -5.44 kcal/mole

 

                    5'-AGTTGACTCTTTCCCCAACTA-3'

                       |||xxx||x|||||||x

                 3'-ATGTCACAAGAAAAAGGGGG-5'

 

GATA7C06-R TAGGATTTTACTATATTCCCCAGG versus GATA41G07-R AAAATCCTTTCAACAAATAGAAGC

Matches = 8

Score = 7

AAAATCCTN

est. tm =  12.1 oC

DeltaG @37 degrees =  -4.79 kcal/mole

 

                        5'-AAAATCCTTTCAACAAATAGAAGC-3'

                           ||||||||x

         3'-GGACCCCTTATATCATTTTAGGAT-5'

 

GATA6G12-F AGCCTGAGCAAAACAGTGAA versus GATA7C06-R TAGGATTTTACTATATTCCCCAGG

Matches = 13

Score = 8

CCTGNGNAANANAGTNAA

est. tm =  less than zero

DeltaG @37 degrees =  -0.28 kcal/mole

 

                    3'-GGACCCCTTATATCATTTTAGGAT-5'

                       ||||x|x||x|x|||x||

                  5'-AGCCTGAGCAAAACAGTGAA-3'

 

ATA29A06N-F AGGCAACATAGTGAGACCCCA versus GATA7C06-R TAGGATTTTACTATATTCCCCAGG

Matches = 14

Score = 7

TNGGNTNTNACTATNTTNCCN

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                     5'-TAGGATTTTACTATATTCCCCAGG-3'

                        |x||x|x|x|||||x||x||x

                     3'-ACCCCAGAGTGATACAACGGA-5'

 

GGAA2A03-F CCTGGGCAACAGAATAAGAT versus GATA7C06-R TAGGATTTTACTATATTCCCCAGG

Matches = 15

Score = 10

CCTGGGNAANANANTAANAT

est. tm =  6.3 oC

DeltaG @37 degrees =  -2.10 kcal/mole

 

                    3'-GGACCCCTTATATCATTTTAGGAT-5'

                       ||||||x||x|x|x|||x||

                    5'-CCTGGGCAACAGAATAAGAT-3'

 

AAAT007-F GCCAACATGGTGAAAACCTAA versus GATA7C06-R TAGGATTTTACTATATTCCCCAGG

Matches = 14

Score = 8

TAGGNTTTNACNATNTTNNC

est. tm =  less than 0 oC

DeltaG @37 degrees =  > 0 kcal/mole

 

                     5'-TAGGATTTTACTATATTCCCCAGG-3'

                        ||||x|||x||x||x||xx|

                    3'-AATCCAAAAGTGGTACAACCG-5'

 

AGAT120-F CTGGGCAACAAAGTGAGACC versus GATA7C06-R TAGGATTTTACTATATTCCCCAGG

Matches = 14

Score = 8

GNTNTNACTNTNTTNCCCAG

est. tm =  less than zero

DeltaG @37 degrees =  -0.19 kcal/mole

 

                    5'-TAGGATTTTACTATATTCCCCAGG-3'

                          |x|x|x|||x|x||x|||||

                       3'-CCAGAGTGAAACAACGGGTC-5'

 

ATA10H11-R CATTCTGGTTGGGGAACATA versus GATA7C06-R TAGGATTTTACTATATTCCCCAGG

Matches = 10

Score = 7

NNTGGGGAANATA

est. tm =  15.1 oC

DeltaG @37 degrees =  -4.85 kcal/mole

 

                    3'-GGACCCCTTATATCATTTTAGGAT-5'

                       xx|||||||x|||

             5'-CATTCTGGTTGGGGAACATA-3'

 

GTTTT001-R AGCCTGGGTGACAGAGTGAA versus GATA7C06-R TAGGATTTTACTATATTCCCCAGG

Matches = 13

Score = 8

CCTGGGNNANANAGTNAA

est. tm =  6.9 oC

DeltaG @37 degrees =  -2.92 kcal/mole

 

                    3'-GGACCCCTTATATCATTTTAGGAT-5'

                       ||||||xx|x|x|||x||

                  5'-AGCCTGGGTGACAGAGTGAA-3'

 

TTAT023z-F TGTTGGGCTTCACCATACCT versus GATA67D03-R GTTACAGTACCTATGGTCATGCC

Matches = 11

Score = 7

GGCNTNACCATANNT

est. tm =  1.2 oC

DeltaG @37 degrees =  -2.45 kcal/mole

 

                    3'-CCGTACTGGTATCCATGACATTG-5'

                       |||x|x||||||xx|

               5'-TGTTGGGCTTCACCATACCT-3'

 

GATA4G12-R GCCATCTGTACTGTTCCCAG versus GATA67D03-R GTTACAGTACCTATGGTCATGCC

Matches = 12

Score = 7

GNNACAGTACNNATGGN

est. tm =  17.0 oC

DeltaG @37 degrees =  -4.51 kcal/mole

 

                    5'-GTTACAGTACCTATGGTCATGCC-3'

                       |xx|||||||xx||||x

                 3'-GACCCTTGTCATGTCTACCG-5'

 

GATA52A12-R AAAAGCCTCCAGTGGGATAT versus TTA032Z-R CCTCAAACTTCCCACAGGAG

Matches = 11

Score = 8

CTCCNGTGGGANNT

est. tm =  17.2 oC

DeltaG @37 degrees =  -5.18 kcal/mole

 

                    3'-GAGGACACCCTTCAAACTCC-5'

                       ||||x||||||xx|

              5'-AAAAGCCTCCAGTGGGATAT-3'

 

GATA52A12-R AAAAGCCTCCAGTGGGATAT versus GATA8G10-R GGCTTTTTCTTCCTTTACCG

Matches = 7

Score = 7

GGCTTTT

est. tm =  7.4 oC

DeltaG @37 degrees =  -4.42 kcal/mole

 

                    5'-GGCTTTTTCTTCCTTTACCG-3'

                       |||||||

       3'-TATAGGGTGACCTCCGAAAA-5'

 

GATA52A12-R AAAAGCCTCCAGTGGGATAT versus ATA7D07-F ATCCCACAGGATGCCTATTT

Matches = 14

Score = 10

ATCCCACNGGANGCNTNT

est. tm =  20.0 oC

DeltaG @37 degrees =  -5.41 kcal/mole

 

                    5'-ATCCCACAGGATGCCTATTT-3'

                       |||||||x|||x||x|x|

                  3'-TATAGGGTGACCTCCGAAAA-5'

 

GATA3H06-R CCTGGGGGTAAGAGGAGATA versus GATA184A08-R GCACCTCTAACCCCCATATT

Matches = 13

Score = 8

NCNCCTCTNACCCCCANN

est. tm =  36.6 oC

DeltaG @37 degrees =  -9.28 kcal/mole

 

                    5'-GCACCTCTAACCCCCATATT-3'

                       x|x|||||x|||||||xx

                  3'-ATAGAGGAGAATGGGGGTCC-5'

 

GATA3H06-R CCTGGGGGTAAGAGGAGATA versus GATA63D12-R AATATTGACTTTCTCTTGCTACCC

Matches = 11

Score = 7

GGGTANNANGAGANA

est. tm =  less than zero

DeltaG @37 degrees =  -1.80 kcal/mole

 

                    3'-CCCATCGTTCTCTTTCAGTTATAA-5'

                       |||||xx|x||||x|

               5'-CCTGGGGGTAAGAGGAGATA-3'

 

GATA62A04-R TAAGGCATGTCTCGGAGCTA versus AGC001b-F ATGCCTTATGGCACTGGACT

Matches = 8

Score = 8

ATGCCTTA

est. tm =  12.8 oC

DeltaG @37 degrees =  -4.98 kcal/mole

 

                    5'-ATGCCTTATGGCACTGGACT-3'

                       ||||||||

        3'-ATCGAGGCTCTGTACGGAAT-5'

 

ATA23A10-R TTCTAGCTTCCCCCTATGCT versus GATA6A05-F CTGCCAGAGAATAGGGTGAA

Matches = 11

Score = 9

TTCNCCCTATNCT

est. tm =  8.0 oC

DeltaG @37 degrees =  -3.51 kcal/mole

 

                    3'-AAGTGGGATAAGAGACCGTC-5'

                       |||x||||||x||

             5'-TTCTAGCTTCCCCCTATGCT-3'

 

ATA23A10-R TTCTAGCTTCCCCCTATGCT versus GATA22G12-F AAAGCTAGAAGGAGCTGGCT

Matches = 9

Score = 8

NAAGCTAGAA

est. tm =  20.0 oC

DeltaG @37 degrees =  -5.79 kcal/mole

 

                    5'-AAAGCTAGAAGGAGCTGGCT-3'

                       x|||||||||

          3'-TCGTATCCCCCTTCGATCTT-5'

 

ATA23A10-R TTCTAGCTTCCCCCTATGCT versus GATA31E08-F AGGGGAGAAGGCTAGAATGA

Matches = 12

Score = 7

ANNGGNGNANGCTAGAA

est. tm =  less than 0 oC

DeltaG @37 degrees =  -0.72 kcal/mole

 

                    5'-AGGGGAGAAGGCTAGAATGA-3'

                       |xx||x|x|x|||||||

                 3'-TCGTATCCCCCTTCGATCTT-5'

 

GATA13G11-R GGGTTCTGCAGAGAAACAGA versus GATA11A11-R TCTGGAAAACCCTAATTCTCC

Matches = 10

Score = 8

TCTGNANAACCC

est. tm =  8.8 oC

DeltaG @37 degrees =  -4.01 kcal/mole

 

                    5'-TCTGGAAAACCCTAATTCTCC-3'

                       ||||x|x|||||

            3'-AGACAAAGAGACGTCTTGGG-5'

 

GATA11A11-R TCTGGAAAACCCTAATTCTCC versus GATA8B01-R ATGGATAAATTGAGTTTCCCC

Matches = 13

Score = 7

NGGNAAACNCNANTTNTCC

est. tm =  less than zero

DeltaG @37 degrees =  -0.75 kcal/mole

 

                     3'-CCCCTTTGAGTTAAATAGGTA-5'

                        x||x||||x|x|x||x|||

                   5'-TCTGGAAAACCCTAATTCTCC-3'

 

GATA165G02N-F CAGGATTTTTCAGAAAGACA versus GATA11A11-R TCTGGAAAACCCTAATTCTCC

Matches = 11

Score = 8

TCTGNAAAANCCTN

est. tm =  13.8 oC

DeltaG @37 degrees =  -4.45 kcal/mole

 

                    5'-TCTGGAAAACCCTAATTCTCC-3'

                       ||||x||||x|||x

              3'-ACAGAAAGACTTTTTAGGAC-5'

 

GATA11A11-R TCTGGAAAACCCTAATTCTCC versus ATA19H08-F TTCCAGAGTATTTGCTTAAGAGG

Matches = 7

Score = 7

TTCCAGA

est. tm =  4.4 oC

DeltaG @37 degrees =  -4.19 kcal/mole

 

                     5'-TTCCAGAGTATTTGCTTAAGAGG-3'

                        |||||||

       3'-CCTCTTAATCCCAAAAGGTCT-5'

 

GGAT3F08-F TTTTCCAGAAGCTCAGATCC versus GATA11A11-R TCTGGAAAACCCTAATTCTCC

Matches = 9

Score = 9

TCTGGAAAA

est. tm =  18.9 oC

DeltaG @37 degrees =  -5.66 kcal/mole

 

                    5'-TCTGGAAAACCCTAATTCTCC-3'

                       |||||||||

         3'-CCTAGACTCGAAGACCTTTT-5'

 

GATA144D04-F AGCCAGAAGGAGGAGGATTA versus GATA11A11-R TCTGGAAAACCCTAATTCTCC

Matches = 8

Score = 7

GGAGNATTA

est. tm =  less than zero

DeltaG @37 degrees =  -1.98 kcal/mole

 

                    3'-CCTCTTAATCCCAAAAGGTCT-5'

                       ||||x||||

         5'-AGCCAGAAGGAGGAGGATTA-3'

 

GATA48E02-R CAGATTTCTGCTTTCCCAGA versus GATA11A11-R TCTGGAAAACCCTAATTCTCC

Matches = 14

Score = 8

TCTGGNAAANCNNAANTCTN

est. tm =  9.9 oC

DeltaG @37 degrees =  -2.88 kcal/mole

 

                    5'-TCTGGAAAACCCTAATTCTCC-3'

                       |||||x|||x|xx||x|||x

                    3'-AGACCCTTTCGTCTTTAGAC-5'

 

AAAT072-R ACTCCACCCAGGAAGTCCA versus GATA88F09-R TTGGGTTTCCTGACTTGTGT

Matches = 13

Score = 7

ACNCNANNCAGGAANNCCA

est. tm =  1.5 oC

DeltaG @37 degrees =  -1.84 kcal/mole

 

                   3'-TGTGTTCAGTCCTTTGGGTT-5'

                      ||x|x|xx||||||xx|||

                   5'-ACTCCACCCAGGAAGTCCA-3'

 

AAAT072-R ACTCCACCCAGGAAGTCCA versus GATA87G01-R TAGATTATCTAAATGGTGGATTTCC

Matches = 8

Score = 7

GGAANTCCA

est. tm =  3.7 oC

DeltaG @37 degrees =  -3.83 kcal/mole

 

                   3'-CCTTTAGGTGGTAAATCTATTAGAT-5'

                      ||||x||||

         5'-ACTCCACCCAGGAAGTCCA-3'

 

AAAT072-R ACTCCACCCAGGAAGTCCA versus ATT023-F CTGGCTGCAGTCTCGTCATA

Matches = 9

Score = 7

CTGGNTGNAGT

est. tm =  less than 0 oC

DeltaG @37 degrees =  -2.88 kcal/mole

 

                   5'-CTGGCTGCAGTCTCGTCATA-3'

                      ||||x||x|||

           3'-ACCTGAAGGACCCACCTCA-5'

 

AAAT072-R ACTCCACCCAGGAAGTCCA versus ATA33B03Z-R TAGTATCCACTTCCTGGGGT

Matches = 11

Score = 7

NNCCCAGGAAGTNNA

est. tm =  24.4 oC

DeltaG @37 degrees =  -6.45 kcal/mole

 

                   3'-TGGGGTCCTTCACCTATGAT-5'

                      xx||||||||||xx|

               5'-ACTCCACCCAGGAAGTCCA-3'

 

TTA032Z-R CCTCAAACTTCCCACAGGAG versus GATA116B01N-F ATGGCCTATTGTGGGATCTT

Matches = 11

Score = 7

AANNTCCCACANNAG

est. tm =  11.3 oC

DeltaG @37 degrees =  -3.83 kcal/mole

 

                    3'-TTCTAGGGTGTTATCCGGTA-5'

                       ||xx|||||||xx||

               5'-CCTCAAACTTCCCACAGGAG-3'

 

ATA50C05-R GCATAATGCACATGCCTGTA versus ATA50C05-R GCATAATGCACATGCCTGTA

Matches = 8

Score = 7

GCATNATGC

est. tm =  8.5 oC

DeltaG @37 degrees =  -4.26 kcal/mole

 

                    5'-GCATAATGCACATGCCTGTA-3'

                       ||||x||||

         3'-ATGTCCGTACACGTAATACG-5'

 

ATA50C05-R GCATAATGCACATGCCTGTA versus GATA43H03N-R ATTTGAACAGAGGCATGTAC

Matches = 10

Score = 7

GNACATGCCTNTN

est. tm =  15.8 oC

DeltaG @37 degrees =  -5.17 kcal/mole

 

                    3'-CATGTACGGAGACAAGTTTA-5'

                       |x||||||||x|x

             5'-GCATAATGCACATGCCTGTA-3'

 

ATA50C05-R GCATAATGCACATGCCTGTA versus GATA25A04-R GTGCATTATGGGGACCATTA

Matches = 10

Score = 9

GTGCATTATGN

est. tm =  28.7 oC

DeltaG @37 degrees =  -7.35 kcal/mole

 

                    5'-GTGCATTATGGGGACCATTA-3'

                       ||||||||||x

           3'-ATGTCCGTACACGTAATACG-5'

 

ATA50C05-R GCATAATGCACATGCCTGTA versus AAT034-R CTGGGATTACAGGCGTGAGT

Matches = 10

Score = 7

NCNCANGCCTGTA

est. tm =  11.3 oC

DeltaG @37 degrees =  -4.43 kcal/mole

 

                    3'-TGAGTGCGGACATTAGGGTC-5'

                       x|x||x|||||||

             5'-GCATAATGCACATGCCTGTA-3'

 

GATA163B10N-R TGGAGACAGCATGTGATTTG versus GATA84E11-R CACACATGCTGTGTCTCTCA

Matches = 12

Score = 7

NNCACATGCTGTNTCNN

est. tm =  32.2 oC

DeltaG @37 degrees =  -8.07 kcal/mole

 

                    5'-CACACATGCTGTGTCTCTCA-3'

                       xx||||||||||x||xx

                 3'-GTTTAGTGTACGACAGAGGT-5'

 

GATA61E03-R GGTATTTTTTGCCCACATGA versus ATA79C10-R GATGAGTGCAAAAAACCACC

Matches = 13

Score = 7

NATGNGNGCAAAAAANNNC

est. tm =  9.2 oC

DeltaG @37 degrees =  -2.38 kcal/mole

 

                    5'-GATGAGTGCAAAAAACCACC-3'

                       x|||x|x||||||||xxx|

                   3'-AGTACACCCGTTTTTTATGG-5'

 

GATA11E02N-R AGTGCTAGACTGGGAGAAGA versus GATA6E05-F ATTGCATGGCAACTCTTCTC

Matches = 7

Score = 7

GAGAAGA

est. tm =  2.1 oC

DeltaG @37 degrees =  -3.57 kcal/mole

 

                    3'-CTCTTCTCAACGGTACGTTA-5'

                       |||||||

       5'-AGTGCTAGACTGGGAGAAGA-3'

 

GATA11E02N-R AGTGCTAGACTGGGAGAAGA versus GATA25A04-F TAGCACTTGAGCACACATGG

Matches = 7

Score = 7

TAGCACT

est. tm =  5.5 oC

DeltaG @37 degrees =  -4.29 kcal/mole

 

                    5'-TAGCACTTGAGCACACATGG-3'

                       |||||||

       3'-AGAAGAGGGTCAGATCGTGA-5'

 

GATA13G11-R GGGTTCTGCAGAGAAACAGA versus ATA28B11-R CGCTCATTTTATCTACCTAACC

Matches = 13

Score = 7

GGTTNNGNAGANAAANNGA

est. tm =  less than zero

DeltaG @37 degrees =  -0.50 kcal/mole

 

                    3'-CCAATCCATCTATTTTACTCGC-5'

                       ||||xx|x|||x|||xx||

                   5'-GGGTTCTGCAGAGAAACAGA-3'

&n