Y-STR Kit Evaluations


Participants: John M. Butler, Amy E. Decker, Peter M. Vallone


Project Timeframe: June 2002 to August 2004


Purpose: As part of developing a new STR kit, prior to its release, commercial manufacturers have supplied us with kit materials for evaluation purposes.


Progress: We evaluated Y-PLEX 5 (ReliaGene), Y-PLEX 12 (ReliaGene), PowerPlex Y (Promega), and Yfiler (Applied Biosystems) Y-STR DNA test kits prior to their release to the general human identity testing community. In the case of ReliaGene, we helped establish nomenclature of alleles and evaluate balance of amplification products (Note: sales of these kits were discontinued by ReliaGene after May 2005 due to patent licensing issues).  Early evaluation of the PowerPlex Y kit was for the purpose of examining the SRM 2395 materials to aid with allele nomenclature confirmation. With the Yfiler kit, we performed a set of requested beta-test experiments and conducted a study with our set of U.S. population samples. These data are available at http://www.cstl.nist.gov/biotech/strbase/NISTpopdata/YfilerNISTdata.xls and are part of the Yfiler Haplotype Database: http://www.appliedbiosystems.com/yfilerdatabase/.



Publications or Presentations Resulting From This Project:

John Butler seminar at California Department of Justice DNA Laboratory (Richmond, CA), March 7, 2007, "Y-STRs: Markers, Mutations, and More" [.pdf]


John Butler workshop with Mike Coble to Northeastern Association of Forensic Scientists (NEAFS) (Rye Brook, NY), November 1, 2006, "Y-Chromosome and Mitochondrial DNA Analysis" [link to workshop webpage]


John Butler talk at Canadian Forensic DNA Technology Workshop (Toronto, Ontario), June 8, 2005, "State of the Y Chromosome: New Advances and State of the Science for Y Chromosome DNA Testing" [.pdf]


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Last updated: 06/27/2007


Disclaimer: This project was supported by National Institute of Justice Grant Number 2003-IJ-R-029, which is an interagency agreement between NIJ and the NIST Office of Law Enforcement Standards, awarded by the National Institute of Justice, Office of Justice Programs, US Department of Justice. Points of view in this document are those of the authors and do not necessarily represent the official position or policies of the US Department of Justice. Certain commercial equipment, instruments and materials are identified in order to specify experimental procedures as completely as possible.  In no case does such identification imply a recommendation or endorsement by the National Institute of Standards and Technology nor does it imply that any of the materials, instruments or equipment identified are necessarily the best available for the purpose.